We narrowed to 12,472 results for: BASE

1. pCN34e-ktrA-yfp

   Plasmid

   #227640

   Purpose

   c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

   Depositor

   Angelika Grundling

   Article

   Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

   Insert

   ktrA-yfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-C-mScarlet-Blast

   Plasmid

   #207711

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Blast cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. 9ao-SOD2-3xHA-TALE(ND4-Left)-Nt.BspD6I(C)

   Plasmid

   #209293

   Purpose

   mitoBE expression vector, target mtND4 gene.

   Depositor

   Wensheng Wei

   Article

   Yi et al Nat Biotechnol. 2023 May 22. doi: 10.103

   Insert

   SOD2-3xHA-TALE(ND4-Left)-Nt.BspD6I(C)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDV05.2_pEF1a-iRFP720_secreted

   Plasmid

   #200409

   Purpose

   Expresses a secreted version of iRFP720 constitutively in mammalian cells

   Depositor

   James Collins

   Article

   Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

   Insert

   iRFP720 (secreted)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. 19ag-SOD2-3xHA-TALE(RNR2-Left)-MutH

   Plasmid

   #209294

   Purpose

   mitoBE expression vector, target mtRNR2 gene.

   Depositor

   Wensheng Wei

   Article

   Yi et al Nat Biotechnol. 2023 May 22. doi: 10.103

   Insert

   SOD2-3xHA-TALE(RNR2-Left)-MutH
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. MscL(G22S)-c-term-cpGFP-ERexp

   Plasmid

   #195346

   Purpose

   Control plasmid for the MscL tension biosensor

   Depositor

   Allen Liu

   Article

   Hsu et al ACS Sens. 2023 Jan 27;8(1):12-18. doi: 1

   Insert

   MscL(G22S)-c-term-cpGFP-ERexp
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMVd2

   Available since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. AIO-GFP

   Plasmid

   #74119

   Purpose

   All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and EGFP-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing

   Depositor

   Steve Jackson

   Article

   Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-4mtD3cpv

   Plasmid

   #36324

   Depositor

   Amy Palmer, Roger Tsien

   Article

   Palmer et al Chem Biol. 2006 May;13(5):521-30.

   Insert

   4mtD3cpv second generation cameleon (calcium sensor) targeted to mitochondria
   

   Use

   Tags

   First 29 aa of human COX subunit 8a

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Aug. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pST5552

   Plasmid

   #36255

   Purpose

   Mycobacteria replicating vector expressing eGFP.

   Depositor

   Carolyn Bertozzi, Jessica Seeliger

   Article

   Seeliger et al PLoS One. 2012;7(1):e29266. Epub 2012 Ja

   Insert

   eGFP
   

   Use

   Synthetic Biology; Mycobacteria replicating vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   ModifiedHsp60

   Available since

   May 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. AIO-mCherry

    Plasmid

    #74120

    Purpose

    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and mCherry-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing

    Depositor

    Steve Jackson

    Article

    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. CAG FUCCI

    Plasmid

    #175266

    Purpose

    Bicistronic FUCCI construct driven by a CAG promoter. Contains I-SceI sites for transgenesis

    Depositor

    James Monaghan

    Article

    Duerr et al Development. 2022 Mar 15;149(6). pii: 27

    Insert

    FUCCI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC-DEST-hPROX1-tdTomato-PuroTK

    Plasmid

    #141027

    Purpose

    Reporter integration

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET24b-Amg2

    Plasmid

    #199434

    Purpose

    expresses Amg2 in bacterial cells

    Depositor

    Andrew Woolley

    Article

    Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

    Insert

    Amg2
    

    Use

    Tags

    his tag

    Expression

    Bacterial

    Mutation

    L159K mutation

    Promoter

    T7

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPRIME-CMV-Neo-recipient

    Plasmid

    #11659

    Purpose

    3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling Neomycin expression

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Neo

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. qTAG-N-Blast-miniTurbo

    Plasmid

    #207681

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-miniTurbo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pSP6-EBNA(2A+DBD)

    Plasmid

    #98749

    Purpose

    In vitro transcription of modified EBNA1 mRNA

    Depositor

    Jim Vadolas, Sara Howden

    Article

    Howden et al Hum Gene Ther. 2006 Aug;17(8):833-44.

    Insert

    Modified EBNA1
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    SP6

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGolden-Neo

    Plasmid

    #51422

    Purpose

    Contain one loxP-PGK-Neo-pA-loxP cassette, used for golden-gate reaction

    Depositor

    Yonglun Luo

    Article

    Luo et al Biotechniques. 2014 May 1;56(5):263-8. d

    Insert

    PGK1 phosphoglycerate kinase 1 (PGK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSGAb-spe

    Plasmid

    #122000

    Purpose

    A sgRNA expression plasmid for genome editing in Acinetobacter baumannii

    Depositor

    Quanjiang Ji

    Article

    Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

    Insert

    none
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV hUbC-dCas9 VP64-T2A-GFP

    Plasmid

    #53192

    Purpose

    Co-expresses human optimized S. pyogenes dCas9-VP64 and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized dead Cas9 VP64 T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Promoter

    hUbC

    Available since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. p663-UBC-miniTurbo-V5-CDKL5_IDG-K

    Plasmid

    #170578

    Purpose

    Gateway destination clone of CDKL5 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-CDKL5 (CDKL5 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    E854G- please see depositor comments

    Promoter

    Ubiquitin

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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