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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,136 results for: BASE;

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Showing: 3201 - 3220 of 13136 results

  2. pENTR-EUI

    Plasmid
    #111835
    Purpose
    Entry vector for adenoviral expression of Ecdysone receptor-based inducible transgene expression switch.
    Depositor
    Hyunju Ro
    Article
    Lee et al Mol Ther Nucleic Acids. 2016 Sep 27;5(9)
    Insert
    GAL4-minimal VP16-EcR (EcR Synthetic)
    Use
    Entry vector for expression
    Promoter
    CMV, 10XUAS
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    111835-plasmid-map-sequence-id-218214

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  5. pSbi-pur-KSR1-CA3-EGFP (C-KSR)

    Plasmid
    #217768
    Purpose
    Fluorescent reporter for ceramide (for stable mammalian expression)
    Depositor
    Paula Nunes-Hasler
    Article
    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do
    Insert
    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    Use
    Sleeping beauty transposase-compatible genome ins…
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    CA3 domain aa 317-400 plus vector-based linker LE…
    Promoter
    EF-1alpha promoter
    Available Since
    June 10, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    217768-plasmid-map-sequence-id-435794

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  6. pETM11-SUMO3-KSR1-CA3-sfGFP (C-KSR)

    Plasmid
    #217766
    Purpose
    Fluorescent reporter for ceramide (for bacterial expression and purification)
    Depositor
    Paula Nunes-Hasler
    Article
    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do
    Insert
    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    Use
    Protein purification (6xhis tag, tev site, sumo3 …
    Tags
    sfGFP
    Expression
    Bacterial
    Mutation
    CA3 domain aa 317-400 plus vector-based linker LE…
    Promoter
    T7 promoter
    Available Since
    June 10, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    217766-plasmid-map-sequence-id-435787

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  9. RANLS-LEHD-BNES

    Plasmid
    #60973
    Purpose
    Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-9 biosensor, used together with plasmid 2 (BNLS).
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddRFP A and ddRFP B
    Tags
    Caspase9 substrate sequence (LEHD) is after NLS s…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. pEDA5_GFPmut3_Y66H

    Plasmid
    #80085
    Purpose
    Positive control plasmid for Nicking Mutagenesis. Constitutively expresses a non-fluorescent variant of GFPmut3 (Y66H) that is mutated to fluorescent (H66Y) in the protocol.
    Depositor
    Timothy Whitehead
    Article
    Wrenbeck et al Nat Methods. 2016 Nov;13(11):928-930. do
    Insert
    GFPmut3_Y66H
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    changed Tyrosine 66 to Histidine
    Promoter
    proB
    Available Since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    80085-plasmid-map-sequence-id-197645

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Showing: 3201 - 3220 of 13136 results