We narrowed to 12,212 results for: HAL;

1. II801: pMVP (L5-L4) ecDHFR DD

   Plasmid

   #121721

   Purpose

   pMVP L5-L4 entry plasmid, contains ecDHFR DD for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term ecDHFR degron domain (stabilized by TMP) to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   ecDHFR Degron Domain (open)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. TU#1806_CRISPR_unc-73_exon21

   Plasmid

   #82360

   Purpose

   to create unc-73E null allele

   Depositor

   Martin Chalfie

   Article

   Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

   Insert

   Cas9 and sgRNA against unc-73 exon21 (unc-73 Nematode)
   

   Use

   CRISPR

   Expression

   Worm

   Promoter

   U6

   Available Since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. KJ801: pMVP/Hygro-DEST

   Plasmid

   #121858

   Purpose

   pMVP destination vector, empty expression vector backbone w/ Hygro selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Pmvp gateway destination vector

   Expression

   Mammalian

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. p3E-Dnm2b-DN

   Plasmid

   #109574

   Purpose

   Multisite gateway vector for 3' tagging with dominant negative Dynamin2b

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   Dynamin2b (dnm2b Zebrafish)
   

   Use

   Zebrafish plasmids

   Mutation

   dominant negative

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. p3E-EHD1b-dEH

   Plasmid

   #109567

   Purpose

   Multisite gateway vector for 3' tagging with EHD1b-delta-EH

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   EHD1b (ehd1b Zebrafish)
   

   Use

   Zebrafish plasmids

   Mutation

   delta-EH

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. LB801: pMVP/PB/eCFP-DEST

   Plasmid

   #121872

   Purpose

   pMVP destination vector, empty PiggyBac transposon vector backbone w/ eCFP selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Piggybac transposon, pmvp gateway destination vec…

   Expression

   Mammalian

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. PX458_MBD1_iso1_1

   Plasmid

   #86303

   Purpose

   Encodes gRNA for 3' target of human MBD1_iso1

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA against MBD1_iso1 (MBD1 Human)
   

   Use

   CRISPR

   Available Since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. KU701: pMVP (L5-L4) eGFP

   Plasmid

   #121707

   Purpose

   pMVP L5-L4 entry plasmid, contains eGFP for 4-component MultiSite Gateway Pro assembly. Allows N-term eGFP fusion to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   eGFP (open)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFETCh_ZNF652

   Plasmid

   #86270

   Purpose

   Donor vector for 3' FLAG tag of human ZNF652

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   ZNF652 homology arms (ZNF652 Human)
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Available Since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. IO901: pMVP (L5-L4) BioID2

    Plasmid

    #121724

    Purpose

    pMVP L5-L4 entry plasmid, contains BioID2 for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term BioID2 to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    BioID2 (open)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Pub-mCD8:GFP-T2A-DsRed:NLS-SV40

    Plasmid

    #130665

    Purpose

    Plasmid to test the efficiency of T2A multiplexing in Aedes aegypti

    Depositor

    Leslie Vosshall

    Article

    Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

    Insert

    AaegPUb-mCD8-GFP-T2A-dsRed-NLS-SV40
    

    Use

    Ae. aegypti expression

    Expression

    Insect

    Promoter

    Aedes aegypti poly-ubiquitin

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. JL302: pMVP/Puro-DEST

    Plasmid

    #121856

    Purpose

    pMVP destination vector, empty expression vector backbone w/ Puro selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Pmvp gateway destination vector

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. sh-Myocardin

    Plasmid

    #100768

    Purpose

    expression of shRNA targeting MYOCD

    Depositor

    Joseph Gordon

    Article

    Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

    Insert

    sh-Myocardin
    

    Use

    RNAi

    Promoter

    H1

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. JD404: pMVP (L3-L2) BioID2 + WPRE

    Plasmid

    #121797

    Purpose

    pMVP L3-L2 entry plasmid, contains BioID2 + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term BioID2 to gene of interest in lentivirus vectors.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    BioID2 + WPRE
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. KJ201: pMVP (R4-R3) osTir1 open

    Plasmid

    #121743

    Purpose

    pMVP R4-R3 entry plasmid, contains osTir1 (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    osTir1 (open)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    9x myc epitope tag

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTAtUbq3 (GB0219)

    Plasmid

    #68192

    Purpose

    Provides the Arabidopsis thaliana polyubiquitin 3 terminator as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtUbq3 terminator
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. PX458_HLF_2

    Plasmid

    #86302

    Purpose

    Encodes gRNA for 3' target of human HLF

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against HLF (HLF Human)
    

    Use

    CRISPR

    Available Since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFETCh_HLF

    Plasmid

    #86262

    Purpose

    Donor vector for 3' FLAG tag of human HLF

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    HLF homology arms (HLF Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. PX458_HLF_1

    Plasmid

    #86301

    Purpose

    Encodes gRNA for 3' target of human HLF

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against HLF (HLF Human)
    

    Use

    CRISPR

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTYB3-human PUM1-HD PUF531_Q867E-Q939E-C935S-Q1011E- C1007S-intein-CBD

    Plasmid

    #73306

    Purpose

    Expresses mutant human PUM1-HD

    Depositor

    Traci Hall

    Article

    Wang et al Nat Methods. 2009 Nov;6(11):825-30. doi:

    Insert

    human PUM1 Pumilio homology domain (PUM1 Human)
    

    Tags

    intein

    Expression

    Bacterial

    Mutation

    Q867E-Q939E-C935S-Q1011E-C1007S

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only