We narrowed to 4,802 results for: pcr
-
-
LTB4R-DuET
Plasmid#213333PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
CRHR1-DuET
Plasmid#213215PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CXCR1-DuET
Plasmid#213218PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRF1 CTFdeltaTA-FLAG
Plasmid#217727PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRF1 (ADGRF1 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-572Available SinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
Clim-DC2-NMM-tdtomato
Plasmid#129557PurposepClimDC-NMM:tdT cell membrane markerDepositorInsertN-Myristoylation motif (NMM) fused to tdtomato
ExpressionBacterialAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR119
Plasmid#106040PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR119; Opto-GPR119; D6)DepositorInsertOpto-GPR119 (GPR119 Human, Bovine)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLG1-puro-sgATL1-5
Plasmid#109007PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorAvailable SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
p172 Dync1i2.E (Ex 1b)
Plasmid#26442DepositorInsertcytoplasmic dynein 1 intermediate chain 2 isoform E (exon 1b) (Dync1i2 Mouse)
UseSubcloning and sequencingMutationN/AAvailable SinceMarch 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
p171 Dync1i1.F
Plasmid#26441DepositorInsertcytoplasmic dynein 1 intermediate chain 1 isoform F (Dync1i1 Mouse)
UseSubcloning and sequencingMutationN/AAvailable SinceMarch 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
2. p167 Dync1i1.D
Plasmid#26439DepositorInsertcytoplasmic dynein 1 intermediate chain 1 isoform D (Dync1i1 Mouse)
UseSubcloning and sequencingMutationN/AAvailable SinceMarch 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB-HaloDAmut-IRES-EGFP-CAAX
Plasmid#234411PurposeExpresses the chemigenetic dopamine (DA) control sensor GRAB_HaloDAmut and a membrane-localized EGFP in mammalian cellsDepositorInsertGPCR activation based chemigenetic dopamine (DA) control sensor GRAB_HaloDAmut
ExpressionMammalianPromoterCMVAvailable SinceJune 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-14-GINIP-Nluc W139A
Plasmid#223557PurposePlasmid expressing GINIP-Nluc W139A in mammalian cellsDepositorInserthuman GINIP W139A - linker(GGGS) - Nluc
Tags3xFLAGExpressionMammalianMutationW139A mutation in GINIP sequenceAvailable SinceMarch 28, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3_attR1-ORF-attR2-NTEV-TCS-GV-2xHA_DEST
Plasmid#194385PurposeGateway Destination vector for split TEV assaysDepositorInsertattR1-ORF-attR2-NTEV-TCS-GV-2xHA
Tags2xHAExpressionMammalianPromoterCMVAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
MBP-rPKL WT
Plasmid#107156PurposeMBP-rPKL WT for production and purification of rat PKL WTDepositorInsertMBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
TagsC-term 6-his and N-term MBPExpressionBacterialPromoterPBADAvailable SinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
MBP-rPKM2 WT
Plasmid#107158PurposeMBP-rPKM2 WT for production and purification of rat PKM2 WTDepositorInsertMBP-tagged rat pyruvate kinase M2 isoform (Pkm Rat)
TagsC-term 6-his and N-term MBPExpressionBacterialPromoterPBADAvailable SinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR61
Plasmid#106027PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR61; Opto-GPR61; C5)DepositorInsertOpto-GPR61 (GPR61 Human, Bovine)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 2xHA GPR153
Plasmid#226812PurposeTet-inducible lentiviral vector for 2xHA GPR153 expressionDepositorInsertGPR153 (GPR153 Human)
UseLentiviralTags2xHA tag followed by a GDPPVAT linkerMutationSequence optimized from NP_997253.2Available SinceMay 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
Hygro-P2A-HA-FKBP-WAPL
Plasmid#217666PurposeRepair template to endogenously tag WAPL with Hygro-P2A-HA-FKBPDepositorInsertWapI (WAPL Human)
TagsdTag (FKBP36V protein)ExpressionBacterial and MammalianPromoterLacAvailable SinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CMV-PdCO-EGFP
Plasmid#198512PurposeExpresses optimized PdCO in frame with EGFP under control of CMV promotor.DepositorInsertPdCO
TagsEGFP and Rho1D4ExpressionMammalianPromoterCMVAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only
