We narrowed to 23,585 results for: lenti

1. pC0056 - LwCas13a-msfGFP-NES

   Plasmid

   #105815

   Purpose

   Expresses active LwaCas13a-NES for mammalian RNA targeting

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   LwCas13a
   

   Use

   CRISPR and Lentiviral

   Tags

   NES and msfGFP-NES-3xHA

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Feb. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMuLE_EXPR_CMV-eGFP_TOP-iRFP_PTCH1-mTurquoise2_CBF-tdTomato

   Plasmid

   #113865

   Purpose

   Triple pathway reporter, 3P-Fluor; wnt-iRFP, hedgehog-mTurquoise2, notch-tdTomato; plus CMV-eGFP. Gateway expression vector for lentivirus generation.

   Depositor

   Manfred Ogris

   Article

   Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

   Inserts

   CMV-eGFP
   TOP-iRFP
   PTCH1-mTurquoise2
   CBF-tdTomato
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC035 - dLwCas13a-msfGFP

   Plasmid

   #91925

   Purpose

   Expresses catalytically inactive LwCas13a for mammalian RNA targeting

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   dLwCas13a
   

   Use

   CRISPR

   Tags

   NLS-3xHA and NLS-msfGFP

   Expression

   Mammalian

   Mutation

   R488A and R1060A

   Promoter

   EF-1a

   Available Since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLL3.7m-psMEK1tight

   Plasmid

   #89362

   Purpose

   A lenti-viral vector expressing photoswitchable MEK1 with minimum basal activity in mammalian cells

   Depositor

   Michael Lin

   Article

   Zhou et al Science. 2017 Feb 24;355(6327):836-842.

   Insert

   psMEK1tight (MAP2K1 Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   Human influenza hemagglutinin (HA) tag and pdDron…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. PLX306 iCre tetO tomato rtTA

   Plasmid

   #158050

   Purpose

   lenti-viral construct with Cre recombinase and doxycycline inducible expression of tdTomato

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Insert

   tdTomato
   

   Tags

   none

   Expression

   Mammalian

   Promoter

   tetO promoter

   Available Since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO-Cre stuffer v3

   Plasmid

   #158030

   Purpose

   lenti-viral construct with Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Sept. 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pLL3.7m-psMEK1

   Plasmid

   #89361

   Purpose

   A lenti-viral vector expressing photoswitchable MEK1 in mammalian cells

   Depositor

   Michael Lin

   Article

   Zhou et al Science. 2017 Feb 24;355(6327):836-842.

   Insert

   psMEK1 (MAP2K1 Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   Human influenza hemagglutinin (HA) tag and pdDron…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cas9-sgRNA-A

   Plasmid

   #149369

   Purpose

   Expresses SpCas9 and sgRNA targeting the lenti-CDDR reporter

   Depositor

   Tarek Abbas

   Article

   Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

   Inserts

   Cas9
   sgRNA-A
   Puromycin resistance
   

   Use

   CRISPR

   Tags

   3xFLAG, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Promoter

   CBh, PGK, and U6

   Available Since

   Nov. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLL3.7m-psRaf1

   Plasmid

   #89364

   Purpose

   A lenti-viral vector expressing photoswitchable Raf1 in mammalian cells

   Depositor

   Michael Lin

   Article

   Zhou et al Science. 2017 Feb 24;355(6327):836-842.

   Insert

   psRaf1 (RAF1 Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   Human influenza hemagglutinin (HA) tag and pdDron…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. JPF0455

    Plasmid

    #124040

    Purpose

    Encodes pPGK1 driving expression of cytoplasmic tagBFP in a 2nd gen. Lenti Virus destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    pHR_pPGK1-NES-tagBFP
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. JPF0456

    Plasmid

    #124041

    Purpose

    Encodes pPGK1 driving expression of mScarlet in a 2nd gen. Lenti Virus destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    pHR_pPGK1-mScarlet
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO-CreERT2 stuffer v3

    Plasmid

    #158046

    Purpose

    lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Oct. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pMuLE_EXPR_CMV-eGFP_TOP-NLuc1.1_12GLI-FLuc_CBF-GLuc

    Plasmid

    #113862

    Purpose

    Triple pathway reporter, 3P-Luc; wnt-NLuc1.1, hedgehog-FLuc, notch-GLuc; plus CMV-eGFP. Gateway expression vector for lentivirus generation.

    Depositor

    Manfred Ogris

    Article

    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

    Inserts

    CMV-eGFP
    TOP-NLuc1.1
    12GLI-Fluc
    CBF-GLuc
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLKO-Cre stuffer v4

    Plasmid

    #158032

    Purpose

    lenti-viral construct with Cre recombinase and U6 driven sgRNA casette with modified tracrRNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Sept. 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. dCas9-hHDAC3

    Plasmid

    #98591

    Purpose

    3rd generation lenti vector encoding dCas9-hHDAC3 (EF1a-NLS-dCas9(N863)-hHDAC3). Expresses dCas9 fused to human HDAC3.

    Depositor

    Zhaolan Zhou

    Article

    Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10

    Insert

    dCAS9-HDAC3 (HDAC3 Human, Synthetic, S. pyogenes)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1A

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-EF1a-mTagBFP2-2A-Puro_hU6-DJ-DR36-BsmBI

    Plasmid

    #226009

    Purpose

    DjCas13d guide RNA cloning backbone (lenti)

    Depositor

    Silvana Konermann

    Article

    Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Cas9-sgRNA-A+B

    Plasmid

    #149370

    Purpose

    Expresses SpCas9 and two sgRNA targeting the lenti-CDDR reporter at two distal sites

    Depositor

    Tarek Abbas

    Article

    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

    Inserts

    Cas9
    sgRNA-A
    sgRNA-B
    Puromycin resistance
    

    Use

    CRISPR

    Tags

    3xFLAG, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Promoter

    CBh, PGK, and U6

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MTK0_062

    Plasmid

    #123951

    Purpose

    Encodes the 2nd gen. Lenti Virus GFP dropout destination vector without insulator as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    pHR DEST
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. [pSK467] pLJC5 HA-Depdc5

    Plasmid

    #109343

    Purpose

    Lenti-viral expression of HA-tagged wildtype Depdc5

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV-EF1a-mTagBFP2-2A-Puro_hU6-RfxDR36-BsmBI

    Plasmid

    #226011

    Purpose

    CasRx guide RNA cloning backbone (lenti)

    Depositor

    Silvana Konermann

    Article

    Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge