We narrowed to 7,778 results for: Pet2

1. pET21b-LOV-ipaA L514K L531E

   Plasmid

   #40253

   Depositor

   Brian Kuhlman

   Article

   Lungu et al Chem Biol. 2012 Apr 20;19(4):507-17.

   Insert

   LOV-ipaA L514K L531E
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   L514K; L531E; Jα helix region hybridized (AsLOV2 …

   Promoter

   T7

   Available since

   Sept. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

2. FL MALT1 Catalytic Mutant in pET29b-Strep

   Plasmid

   #48969

   Purpose

   Expresses Full length catalytic mutant MALT1 in e.coli

   Depositor

   Guy Salvesen

   Article

   Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

   Insert

   MALT1 isoform A (MALT1 Human)
   

   Use

   Tags

   His and Strep

   Expression

   Bacterial

   Mutation

   Catalytic Mutant C464A

   Promoter

   T7

   Available since

   Nov. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pET29-hMIGA2(306-593)-6xhis

   Plasmid

   #192837

   Purpose

   Express codon-optimized human MIGA2 fragment 306-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteria

   Depositor

   Karin Reinisch

   Article

   Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

   Insert

   Codon-optimized human Mitoguardin 2 (MIGA2 Human)
   

   Use

   Tags

   6xhis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET22b-ecDHFR-N23PP/L28F/G51PEKN

   Plasmid

   #118585

   Purpose

   Bacterial expression of E.coli DHFR N23PP, L28F, and G51PEKN mutant

   Depositor

   Stephen Benkovic

   Article

   Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

   Insert

   DHFR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N23 mutated to P23, L28 mutated to F28, G51 mutat…

   Promoter

   T7

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a SUMO Nup53xl 162-267

   Plasmid

   #86002

   Purpose

   bacterial expression of his6 and SUMO tagged RRM motif of Xenopus laevis Nup53

   Depositor

   Wolfram Antonin

   Type

   Empty backbone

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a-FgßF3-ddFLN4-HIS-ybbr

   Plasmid

   #101743

   Purpose

   mutant FgßF3 peptide (additional phenylalanine) with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces

   Depositor

   Hermann Gaub

   Article

   Milles et al Science. 2018 Mar 30;359(6383):1527-1533

   Insert

   FgßF3 peptide, ddFLN4 fingerprint (FGB Human, Dictyostelium discoideum)
   

   Use

   Tags

   HIS and ybbr

   Expression

   Bacterial

   Mutation

   C18S in ddFLN4, added 9F in FgßF3

   Promoter

   T7

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. delNC C-His Ana GvpC in pET28a

   Plasmid

   #85736

   Purpose

   Expresses a truncated His-tagged GvpC from Anabaena flos-aquae without the N-and C-terminal regions in E.coli

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

   Insert

   Gas vesicle protein C (truncated and his-tagged)
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Deletion of N and C terminal regions, addition of…

   Promoter

   T7

   Available since

   March 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-ybbR-HIS-CBM-CohI

   Plasmid

   #58709

   Purpose

   Probing molecule to perform single molecule force spectroscopy experiments

   Depositor

   Hermann Gaub

   Article

   Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

   Insert

   CBM-CohI
   

   Use

   Tags

   HIS, HRV 3C, and ybbR

   Expression

   Bacterial

   Mutation

   changed Alanine 2 to Cysteine in CBM domain

   Promoter

   T7

   Available since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28b-NmCas9-Flag D16A+H588A

   Plasmid

   #109033

   Purpose

   D16A+H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

   Depositor

   Yan Zhang

   Article

   Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

   Insert

   CRISPR-associated endonuclease Cas9
   

   Use

   Tags

   Flag Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-Hook3 I136A, I139A, M142A

    Plasmid

    #74617

    Purpose

    Expresses a triple mutant truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239, I136A, I139A, M142A (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    D102A, E108A

    Promoter

    T7

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28b(+)_S. cerevisiae Hbs1(166-611)

    Plasmid

    #39338

    Depositor

    SeWon Suh

    Insert

    Elongation factor 1 alpha-like protein
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pET28a-ybbR-HIS-10FNIII(x4)-DocI

    Plasmid

    #58712

    Purpose

    Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

    Depositor

    Hermann Gaub

    Article

    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

    Insert

    10FNIII(x4)-DocI
    

    Use

    Tags

    HIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a-His6-GFP-UROD-N367A

    Plasmid

    #236742

    Purpose

    Protein overexpression of eGFP-human uroporphyrinogen decarboxylase N367A

    Depositor

    Christina Woo

    Article

    Lloyd et al Cell Chem Biol. 2025 Aug 5:S2451-9456(25

    Insert

    eGFP-Uroporphyrinogen decarboxylase human N367A (UROD Human, Synthetic)
    

    Use

    Tags

    His6 and eGFP

    Expression

    Bacterial

    Mutation

    N367A

    Promoter

    Available since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET21a asyn(Δ41-54/Δ103-130)

    Plasmid

    #242236

    Purpose

    Expresses alpha synuclein (missing exon 3 and 5, residues 41-54 and 103-130) in bacteria

    Depositor

    Jennifer Lee

    Article

    SanGiovanni et al Protein Sci. 2025 Jul;34(7):e70195. doi:

    Insert

    asyn(Δ41-54/Δ103-130)
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    deleted amino acids 41-54 and 103-130

    Promoter

    Available since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET21a asyn(66-140) K96A/K97A

    Plasmid

    #240590

    Purpose

    Overexpress truncated alpha-Synuclein protein

    Depositor

    Jennifer Lee

    Article

    McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

    Insert

    aSyn (SNCA Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 66-140 only, K96A, K97A

    Promoter

    T7

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET21a asyn(66-140) E104A/E105A

    Plasmid

    #240589

    Purpose

    Overexpress truncated alpha-Synuclein protein

    Depositor

    Jennifer Lee

    Article

    McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

    Insert

    aSyn (SNCA Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 66-140 only, E104A, E105A

    Promoter

    T7

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET21a asyn(66-140) E83K/K97E

    Plasmid

    #240591

    Purpose

    Overexpress truncated alpha-Synuclein protein

    Depositor

    Jennifer Lee

    Article

    McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

    Insert

    aSyn (SNCA Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 66-140 only, E83K, K97E

    Promoter

    T7

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET21a asyn(66-140) K80E/E83K

    Plasmid

    #240593

    Purpose

    Overexpress truncated alpha-Synuclein protein

    Depositor

    Jennifer Lee

    Article

    McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

    Insert

    aSyn (SNCA Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 66-140 only, E83K, K80E

    Promoter

    T7

    Available since

    Aug. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET21a asyn(66-140) E83K/K96E

    Plasmid

    #240592

    Purpose

    Overexpress truncated alpha-Synuclein protein

    Depositor

    Jennifer Lee

    Article

    McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

    Insert

    aSyn (SNCA Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 66-140 only, E83K, K96E

    Promoter

    T7

    Available since

    Aug. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET21b(+)_SARS-CoV-2_3CLpro_Q306A-HIS

    Plasmid

    #224697

    Purpose

    Bacterial Expression and Purification of SARS-CoV-2 3CLpro active protease with His-tag

    Depositor

    Christopher Overall

    Article

    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

    Insert

    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    

    Use

    Tags

    Gly-Gly-6xHis

    Expression

    Bacterial

    Mutation

    silent mutation C to T at position 10546 (elimina…

    Promoter

    T7

    Available since

    July 2, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge