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We narrowed to 25,508 results for: crispr

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Showing: 3241 - 3260 of 25508 results

  4. pSc1-DD

    Plasmid
    #80439
    Purpose
    Expresses eGFP with ecDHFR along with an U6 promoter driven gRNA which cleaves the plasmid in-cell
    Depositor
    Ervin Welker
    Publication
    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    sp Cas9 gRNA
    eGFP
    Use
    CRISPR
    Tags
    E. coli dihydrofolate reductase
    Expression
    Mammalian
    Promoter
    CMV and U6
    Available since
    Aug. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80439-plasmid-map-sequence-id-151455

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  10. pLentiRNACRISPR_011-TetO-NLS-inactive-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid
    #228557
    Purpose
    Expresses inactive RfxCas13d in mammalian cells for binding of target RNAs in combination with compatible crRNAs. Localized to the nucleus
    Depositor
    Neville Sanjana
    Publication
    Hart et al Nat Biotechnol. 2025 Feb 11. doi: 10.103
    Insert
    dRfxCas13d
    Use
    Lentiviral
    Tags
    NLS-HA
    Mutation
    R239A/H244A/R858A/H863A
    Available since
    Aug. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    228557-plasmid-map-sequence-id-456689

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  11. pGL3-sgACSL3.C-Cas9-P2A-Puro

    Plasmid
    #129412
    Purpose
    Encoding Cas9 and sgACSL3.C for CRISPR/Cas9 mediated HDR tagging of endogenous human ACSL3 C-terminus
    Depositor
    Elina Ikonen
    Publication
    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 and sgACSL3.C (ACSL3 Human)
    Use
    CRISPR
    Tags
    P2A-Puro
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    Aug. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    129412-plasmid-map-sequence-id-254823

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  15. pGG422_UV5

    Plasmid
    #165607
    Purpose
    Vector for expression of the SpCas9 KG variant with sgRNA in E. coli: KG(SpCas9, D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)
    Depositor
    Marcus Noyes
    Publication
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    lacUV5 driving Streptococcus pyogenes Cas9 KG(D1332K/R1333G) and hEGFP-sgRNA
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Mutation
    D1332K and R1333G mutations in SpCas9
    Promoter
    lacUV5 driving Cas9 KG and UV5 driving sgRNA
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165607-plasmid-map-sequence-id-330962

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  18. eSpCas9-hGeminin

    Plasmid
    #199344
    Purpose
    modified version of the eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA plasmid (addgene #86613) where the C-terminus of the eSpCas9 enzyme was fused to amino acids 1 – 110 of human Geminin
    Depositor
    Iain Hagan
    Publication
    Morrison et al Open Biol. 2023 Apr;13(4):230021. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATP1A1 G3 sgRNA+user-specified sgRNA+enhanced specificity Cas9 (1.1) (addgene 86613) with cas9 fused to hgem fragment 1-110) (GMNN S. pyogenes)
    Tags
    cas9 c-term fused to hgemenin 1-110 fragment
    Expression
    Mammalian
    Mutation
    cas9 c-term fused to hgemenin 1-110 fragment
    Promoter
    cbh
    Available since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    199344-plasmid-map-sequence-id-402934

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Showing: 3241 - 3260 of 25508 results