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We narrowed to 63,044 results for: cloning

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Showing: 3261 - 3280 of 63044 results

  1. VVT1

    Plasmid
    #65779
    Purpose
    The Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNA
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    SaCas9 gRNA backbone, without spacer sequence
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    65779-plasmid-map-sequence-id-112660

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  3. p415 TEF Su9‐roGFP2‐Tsa2ΔCR

    Plasmid
    #83240
    Purpose
    The genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For mitochondrial matrix expression.
    Depositor
    Tobias Dick
    Publication
    Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do
    Insert
    Su9-roGFP2-Tsa2dCr
    Tags
    roGFP2 fusion with Tsa2dCr with Su9 targetting se…
    Expression
    Yeast
    Promoter
    TEF
    Available since
    Nov. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    83240-plasmid-map-sequence-id-518142

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  9. pMAL-c5x_UnaG

    Plasmid
    #163125
    Purpose
    A plasmid with UnaG coding sequence cloned into it. UnaG gene codon optimized for E. coli and B. theta. Expresses UnaG with N-term maltose-binding protein (MBP)-tag and C-term His-tag.
    Depositor
    Julie Biteen
    Publication
    Chia et al Curr Res Microb Sci. 2020 Sep;1:1-6. doi
    Insert
    UnaG
    Tags
    His and MBP
    Expression
    Bacterial
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    163125-plasmid-map-sequence-id-324911

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  12. pINDUCER dCas9-TET1CDMut

    Plasmid
    #101920
    Purpose
    The dCas9-TET1CDMut fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948). The TET1 catalytic domain is mutated to abolish catalytic function.
    Depositor
    Danwei Huangfu
    Publication
    Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10
    Insert
    dCas9-TET1CD Mut
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Mutated TET1 catalytic domain
    Available since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    101920-plasmid-map-sequence-id-194554

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  13. AAV-pCbh-SpRY[C-term]-gRNAentry (CA20)

    Plasmid
    #197511
    Purpose
    Cbh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpRY and BsmBI entry cassette to clone SpCas9 gRNA spacer
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    AAV-[ITR]-pCbh-BPNLS-NpuC-SpRY[C-term]-BPNLS-gRNA[BsmBI]-pU6-[ITR]
    Use
    AAV and CRISPR
    Tags
    BPNLS and BPNLS-NpuC(intein)
    Mutation
    C-terminal mutations of SpRY(L1111R/D1135L/S1136W…
    Promoter
    Cbh
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197511-plasmid-map-sequence-id-386647

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  14. p2R3a-VenusYFP-3AT

    Plasmid
    #71269
    Purpose
    Entry clone containing Venus. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system
    Depositor
    Ari Pekka Mähönen
    Publication
    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246
    Insert
    VenusYFP
    Use
    Gateway Cloning
    Tags
    4xGly linker and T3A pea Pisum sativum ribulose-1…
    Available since
    Dec. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    71269-plasmid-map-sequence-id-129809

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  17. pcDNA3.1_hHNF4alpha

    Plasmid
    #206878
    Purpose
    The ORF of HNF4α (variant 2) cDNA amplified from the RT human liver RNA was cloned at the Bam HI/XbaI sites of the pcDNA3.1(+), the mammalian expression vector.
    Depositor
    Catharine Ross, Reza Zolfaghari
    Publication
    Zolfaghari et al J Cell Biochem. 2014 Oct;115(10):1740-51
    Insert
    Human hepatocyte nuclear factor 4 alpha (HNF4A Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    206878-plasmid-map-sequence-id-422375

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  18. pUC19-U6-BsmBI_cassette-Nme/Nme2Cas9-sgRNA (KAC32)

    Plasmid
    #133794
    Purpose
    U6 promoter sgRNA entry vector used for all NmeCas9 or Nme2Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Amrani et al. Genome Biology 2018
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Publication
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Insert
    NmeCas9 and Nme2Cas9 sgRNA entry vector
    Expression
    Mammalian
    Mutation
    sgRNA architecture from Amrani et al. Genome Biol…
    Promoter
    U6
    Available since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133794-plasmid-map-sequence-id-268076

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  20. pPbcas13b

    Plasmid
    #89906
    Purpose
    Bacterial expression for pbCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.
    Depositor
    Feng Zhang
    Publication
    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.
    Insert
    Cas13b
    Expression
    Bacterial
    Promoter
    Lac
    Available since
    May 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    89906-plasmid-map-sequence-id-178224

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Showing: 3261 - 3280 of 63044 results