We narrowed to 59,427 results for: cloning

1. KH305: pMVP (R4-R3) βgal open

   Plasmid

   #121739

   Purpose

   pMVP R4-R3 entry plasmid, contains βgal (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   βgal (open)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. IY704: pMVP (L1-R5) EF1a promoter

   Plasmid

   #121693

   Purpose

   pMVP L1-R5 entry plasmid, contains EF1a promoter for 4-component MultiSite Gateway Pro assembly

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   EF1a promoter
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. LF801: pMVP (L1-R5) cTnT promoter

   Plasmid

   #121696

   Purpose

   pMVP L1-R5 entry plasmid, contains cTnT promoter for 4-component MultiSite Gateway Pro assembly

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   cTnT promoter (Cardiomyocyte specific)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. KU801: pMVP (L5-L4) mCherry

   Plasmid

   #121709

   Purpose

   pMVP L5-L4 entry plasmid, contains mCherry for 4-component MultiSite Gateway Pro assembly. Allows N-term mCherry fusion to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   mCherry (open)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. KU901: pMVP (L5-L4) eCFP

   Plasmid

   #121711

   Purpose

   pMVP L5-L4 entry plasmid, contains eCFP for 4-component MultiSite Gateway Pro assembly. Allows N-term eCFP fusion to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   eCFP (open)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. KV001: pMVP (L5-L4) eYFP

   Plasmid

   #121713

   Purpose

   pMVP L5-L4 entry plasmid, contains eYFP for 4-component MultiSite Gateway Pro assembly. Allows N-term eYFP fusion to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   eYFP (open)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiCRISPRv2-mCherry

   Plasmid

   #99154

   Purpose

   Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.

   Depositor

   Agata Smogorzewska

   Inserts

   Cas9
   mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EFS-NS

   Available Since

   Aug. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCW57-MCS1-P2A-MCS2 (Neo)

   Plasmid

   #89180

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

   Depositor

   Adam Karpf

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Doxycycline inducible; p2a self cleav…

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-SFFV-MCS-WPRE-UbC-mCherry

   Plasmid

   #211799

   Purpose

   Lentiviral vector plasmid containing a multiple cloning site (MCS) under the spleen focus-forming virus (SFFV) promoter and expressing mCherry under the Ubiquitin C (UbC) promoter

   Depositor

   Bart Nieuwenhuis, Richard Eva

   Inserts

   multiple cloning site
   mCherry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV and UbC

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. UEV1

    Plasmid

    #25619

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    UEV1 (UBE2V1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 31, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

11. SHMT2

    Plasmid

    #25479

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SHMT2 (SHMT2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    A269T

    Available Since

    Aug. 23, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pDONR221-TMPRSS4

    Plasmid

    #158446

    Purpose

    Gateway Cloning compatible entry vector for the human TMPRSS4 gene.

    Depositor

    Alejandro Chavez, Sho Iketani

    Insert

    TMPRSS4 (TMPRSS4 Human)
    

    Use

    Gateway entry vector

    Available Since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. PLAP

    Plasmid

    #25152

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    plap.454.738 (PLAA Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 6, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

14. plenti guide ds red

    Plasmid

    #128055

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and DsREd from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. NKTR

    Plasmid

    #25597

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    NKTR (NKTR Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 25, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

16. INMT

    Plasmid

    #25475

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    INMT (INMT Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 23, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pFB-CT10HF-LIC

    Plasmid

    #39191

    Purpose

    SGC Baculovirus transfer vector with C-terminal His tag and FLAG tag, preceded by a TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selection

    Depositor

    Nicola Burgess-Brown

    Type

    Empty backbone

    Use

    Baculovirus

    Tags

    TEV-His-FLAG

    Promoter

    polyhedrin

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. SMAP1L

    Plasmid

    #25546

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SMAP1L (SMAP2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 25, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

19. USP04

    Plasmid

    #25223

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    usp04.001.229 (Usp4 Mouse)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 13, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

20. NRDP1

    Plasmid

    #25620

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    NRDP1 (RNF41 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains USP8 interaction Domain

    Available Since

    Aug. 31, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits