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pSSBIO32_dCas9_RPA3701

Plasmid

#240490

Purpose

CRISPRi-mediated Knockdown of RPA3701

Depositor

Rajib Saha

Article

Kathol et al Appl Environ Microbiol. 2025 Aug 20;91(8

Insert

Cas9 (cas9 Streptococcus pyogenes)

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Plac

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pSSBIO32_dCas9_RPA1580

Plasmid

#240488

Purpose

CRISPRi-mediated Knockdown of RPA1580

Depositor

Rajib Saha

Article

Kathol et al Appl Environ Microbiol. 2025 Aug 20;91(8

Insert

Cas9 (cas9 Streptococcus pyogenes)

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Plac

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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hVEGF165-eGFP-hVEGF165 glycosylation sequon-His tag

Plasmid

#240484

Purpose

Express engineered glycosylated eGFP in CHO cells

Depositor

Xi Ren

Article

Xing et al Acta Biomater. 2025 Nov;207:221-232. doi

Insert

glycosylated eGFP

Tags

3 repeats of hVEGF165 glycosylation sequon, 6xHis…

Expression

Mammalian

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Puro-CAG-JEDI-2Psub

Plasmid

#247186

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-2Psub expressed under strong mammalian promoter (CAG)

Depositor

Francois St-Pierre

Article

Carolan et al Nat Commun. 2025 Oct 3;16(1):8834. doi:

Insert

JEDI-2Psub

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 JDM16-GFPnb-mCh

Plasmid

#235667

Purpose

Expresses designed inhibitor of Hsp70 tagged with a GFP nano body and mCherry

Depositor

David Baker

Article

Zhang et al Cell Chem Biol. 2025 Feb 4:S2451-9456(25

Insert

JDM16-GFPnb-mCh (Mch Synthetic)

Tags

GFP nanobody and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pMB.M.ED

Plasmid

#234576

Purpose

Expression of MBP-EED

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

EED (EED Human)

Expression

Insect

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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PX330-sgRNA006_CTCF

Plasmid

#232928

Purpose

Expression vector for a sgRNA against the mouse CTCF locus and SpCas9.

Depositor

Danny Reinberg

Article

Lucero et al bioRxiv, March 19, 2025

Insert

Cas9 (Ctcf S. pyogenes)

Use

CRISPR

Expression

Mammalian

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pLY227

Plasmid

#184148

Purpose

short crRNA generator with spacer LEA2 with WT repeat region

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Insert

s-crRNA-LEA2-WT

Use

Synthetic Biology

Promoter

Plux2

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLY232

Plasmid

#184149

Purpose

short tracrRNA generator with WT anti-repeat region

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Insert

s-tracrRNA-WT

Use

Synthetic Biology

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLY171

Plasmid

#184145

Purpose

sgRNA with 3 RNA aptemers BoxB at the 3'-end for CRISPRa

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Insert

sgRNA-LEA2-ex3

Use

Synthetic Biology

Promoter

Plux2

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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UBC1cer40GEM

Plasmid

#172699

Purpose

Ubiquitous expression of 60meric GEM monomer

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

monomer of 60mer GEM

Use

Unspecified

Tags

GFP

Expression

Plant

Promoter

UBC1

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pET11a-Z-N-DiB2

Plasmid

#168473

Purpose

N-fragment of the DiB2‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168256: pMRBad-Z-C-wtBlc to obtain DiB2‐split-Zip protein

Depositor

Jens Meiler

Article

Bozhanova et al Sci Rep. 2020 Jul 6;10(1):11049. doi: 10

Insert

N-fragment of DiB2 + Leucine Zipper

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab38a

Plasmid

#140887

Purpose

Expression of zebrafish GFP-tagged Rab38a in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab38a (rab38a Zebrafish)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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PW124-LMNA 5'HA-TriTag (mCherry)-3'HA (HDR donor)

Plasmid

#164047

Purpose

CRISPR donor plasmid to insert TriTag (mCherry) into the N-terminus of human LMNA gene

Depositor

Baohui Chen

Article

Xu et al Nucleic Acids Res. 2020 Oct 26. pii: 594

Insert

mCherry-TriTag

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-rab14

Plasmid

#109668

Purpose

Multisite gateway vector for 3' tagging with rab14

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab14 (rab14 Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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PX330_ZBTB7B_2

Plasmid

#135747

Purpose

Encodes gRNA for 3' target of human ZBTB7B

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZBTB7B gRNA (ZBTB7B Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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PX330_ZBTB7B_1

Plasmid

#135746

Purpose

Encodes gRNA for 3' target of human ZBTB7B

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZBTB7B gRNA (ZBTB7B Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pTW065

Plasmid

#115952

Purpose

Destination Vector

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 3'UTR Dest

Use

Synthetic Biology

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pTW103

Plasmid

#115946

Purpose

ORF insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 L7Ae

Use

Synthetic Biology

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pTW086M

Plasmid

#115934

Purpose

ORF insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 mKate

Use

Synthetic Biology

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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