We narrowed to 8,679 results for: BLI;

1. SpCas9_hGEM(1-110)_hSLBP(56-108)

   Plasmid

   #191388

   Purpose

   SpCas9 fused with geminin and SLBP degrons to improve HDR ratio

   Depositor

   Yannick Doyon

   Insert

   hGEM(1-110)_hSLBP(56_108)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLJM60

   Plasmid

   #34610

   Depositor

   David Sabatini

   Insert

   deptor (DEPTOR Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

3. miR-124

   Plasmid

   #182363

   Purpose

   Plasmid with 4x miR-124 target sites

   Depositor

   Mario Capecchi

   Insert

   4x miR-124 target sites
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKLV2-U6(BsmBI)-EF1a-BFP-BSD

   Plasmid

   #117136

   Purpose

   Empty vector encoding BFP-BSD

   Depositor

   Kosuke Yusa

   Insert

   BSD2A
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   modified BFP

   Promoter

   human U6 promoter, human EF1a promoter

   Available since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJK651

   Plasmid

   #71702

   Purpose

   Produces E. coli thioredoxin reductase with a C-terminal His6 tag (EcTrxBH6)

   Depositor

   T. J. Kappock

   Insert

   thioredoxin reductase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. 4MVT

   Plasmid

   #60101

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PIAS3:YTC008-B10:C225910 (PIAS3 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   See comments

   Promoter

   T7

   Available since

   Oct. 22, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

7. CMV-H2B-sfGFP-T2A-iCre

   Plasmid

   #183351

   Purpose

   Histone H2B-sfGFP and iCre recombinase

   Depositor

   Mario Capecchi

   Insert

   Histone H2B-sfGFP-T2A-iCre
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHH0103 FNBP3 WW domain #1

   Plasmid

   #104233

   Purpose

   Bacterial expression of WW domain #1 from FNBP3

   Depositor

   Marius Sudol, Sachdev Sidhu

   Insert

   FNBP3 WW domain #1 (PRPF40A Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available since

   March 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. p221-RV-dlx-dio-Tdtomato

   Plasmid

   #185697

   Purpose

   Retroviral vector expression cre-dependent membrane tagged Tdtomato driven by the mDlx promoter

   Depositor

   Lynette Lim

   Insert

   membrane Tdtomato
   

   Use

   Cre/Lox and Retroviral

   Tags

   Gap43 palmitoylation

   Expression

   Mutation

   Promoter

   mDlx

   Available since

   July 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMCP-GPI Control Plasmid

    Plasmid

    #101132

    Purpose

    Expression of MCP-Tagged GPI anchor in Mammalian cells

    Depositor

    New England Biolabs, Ana Egana

    Insert

    GPI
    

    Use

    Tags

    MCP-tag (Mutant ACP- tag)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. mEos3.2-Vinculin-N-21

    Plasmid

    #66952

    Purpose

    Localization: Focal Adhesions, Excitation: 507 / 572, Emission: 516 / 580

    Depositor

    Michael Davidson

    Insert

    Vinculin (VCL Human)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    V234L in Vinculin

    Promoter

    CMV

    Available since

    July 7, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pUS252b

    Plasmid

    #191831

    Purpose

    Expresses fuGFPb constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuGFPb to place in other plasmid backbones

    Depositor

    Nicholas Coleman

    Insert

    fuGFPb
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pCAGGS-S-B.1.617.2-FLAG

    Plasmid

    #177097

    Purpose

    B.1.617.2 variant S-protein with c-terminal FLAG tag

    Depositor

    Daniel Conway

    Insert

    S-protein B.1.617.2 (S SARS-CoV-2)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. 1782_pAAV-U6-Ai9-Sa-gRNA2-CB-SACas9-HA-OLLAS-spA_C

    Plasmid

    #135978

    Purpose

    gRNA against the right loxP site of the Ai9 allele

    Depositor

    William Lagor

    Insert

    Ai9 gRNA2
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    HA

    Expression

    Mutation

    Promoter

    CB

    Available since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Flag-TRIM24-C840W/F869D/F979A/N980A

    Plasmid

    #28144

    Depositor

    Michelle Barton

    Insert

    TRIM24 (TRIM24 Human)
    

    Use

    Tags

    FLAGX2

    Expression

    Mammalian

    Mutation

    C840W, F869D, F979A, N980A

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

16. 1781_pAAV-U6-Ai9-Sa-gRNA1-CB-SACas9-HA-OLLAS-spA_C

    Plasmid

    #135979

    Purpose

    gRNA against the left loxP site of the Ai9 allele

    Depositor

    William Lagor

    Insert

    Ai9 gRNA1
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    HA

    Expression

    Mutation

    Promoter

    U6

    Available since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. PX381 Filifactor alocis Cas9

    Plasmid

    #68318

    Purpose

    Filifactor alocis Cas9

    Depositor

    Feng Zhang

    Insert

    FaCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. attB Neo* attP

    Plasmid

    #138412

    Purpose

    attB and attP-flanked pPgk-pEM7-Neo*-bpA positive selection cassette

    Depositor

    Mario Capecchi

    Insert

    Neo*
    

    Use

    Mouse Targeting; Att/phic31

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJK217

    Plasmid

    #72471

    Purpose

    Produces Acetobacter aceti 1023 citrate synthase with N-terminal His6 tag (H6AaAarA)

    Depositor

    T. J. Kappock

    Insert

    citrate synthase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL3-alphaMHC 3300bp

    Plasmid

    #51934

    Purpose

    luciferase reporter for rat alpha-MHC promoter

    Depositor

    Jeffery Molkentin

    Insert

    alpha MHC (Myh6 Rat)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mutation

    -2930 to +410 promoter region

    Promoter

    alpha MHC

    Available since

    May 28, 2014

    Availability

    Academic Institutions and Nonprofits only