We narrowed to 10,140 results for: donor

1. Bxb1-GA_CAG-EGFP

   Plasmid

   #229799

   Purpose

   Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of EGFP

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   EGFP
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. ATM-Halo HRD

   Plasmid

   #207082

   Purpose

   Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous ATM locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   HaloTag followed by a PolyA signal and PuroR cassette flanked by human ATM locus sequences
   

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   None

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. AICSDP-50: NPM1-mEGFP

   Plasmid

   #109122

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human NPM1

   Depositor

   Allen Institute for Cell Science

   Insert

   NPM1 Homology Arms with linker-mEGFP (NPM1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   May 4, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. AICSDP-28:TUBA1B-mTagRFP-T

   Plasmid

   #101785

   Purpose

   Homology arms and mTagRFPT-linker sequence for N-terminus (second exon) tagging of human TUBA1B

   Depositor

   Allen Institute for Cell Science

   Insert

   TUBA1B Homology Arms with mTagRFPT-linker (TUBA1B Human)
   

   Use

   CRISPR; Donor template

   Tags

   mTagRFPT-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   Oct. 31, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pJ204-loxP-100RPU.2-coEPO-lox2272

   Plasmid

   #154828

   Purpose

   Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing one copy of erythropoietin

   Depositor

   Lise Marie Grav

   Article

   Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

   Insert

   coEPO
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Mutation

   Codon-optimized (Cricetulus griseus)

   Promoter

   100RPU.2

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. AICSDP-22:CETN2-mTagRFP-T

   Plasmid

   #101781

   Purpose

   Homology arms and mTagRFPT-linker sequence for N-terminus tagging of human CETN2

   Depositor

   Allen Institute for Cell Science

   Insert

   CETN2 Homology Arms with mTagRFPT-linker (CETN2 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mTagRFPT-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   Oct. 31, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. AICSDP-27:SLC25A17-mEGFP

   Plasmid

   #101784

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human SLC25A17

   Depositor

   Allen Institute for Cell Science

   Insert

   SLC25A17 Homology Arms with linker-mEGFP (SLC25A17 Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   Oct. 31, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pSEED-mScarlet3:ALFA

   Plasmid

   #219641

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in mScarlet3:ALFA using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   mScarlet:ALFA SEED Cassette
   

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSEED-mCherry

   Plasmid

   #219657

   Purpose

   Permits GoldenGate (BsmBI) assembly of donor plasmids to knock-in mCherry using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   sfGFP SEED Cassette
   

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSEED-Dendra2

    Plasmid

    #219645

    Purpose

    Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in Dendra2 using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    Dendra2 SEED Cassette
    

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCPP5741

    Plasmid

    #128722

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

    Insert

    shcV-hopV1
    

    Expression

    Bacterial

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCPP5910

    Plasmid

    #128717

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

    Insert

    shcO-hopO1-1
    

    Expression

    Bacterial

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCPP5058

    Plasmid

    #128710

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

    Insert

    shcF-hopF2
    

    Expression

    Bacterial

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSEED-OLLAS:HA

    Plasmid

    #219647

    Purpose

    Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in OLLAS and HA tags using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    OLLAS:HA SEED Cassette
    

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAVS1-PuroR Xlone eGFP

    Plasmid

    #179837

    Purpose

    Doxycycline-inducible expression of eGFP

    Depositor

    Xiaoping Bao

    Article

    Jung et al ACS Synth Biol. 2022 May 24. doi: 10.102

    Insert

    eGFP
    

    Use

    CRISPR, Synthetic Biology, and TALEN ; Donor plas…

    Expression

    Mammalian

    Promoter

    TRE3GS

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. RNF168-Halo HRD

    Plasmid

    #207080

    Purpose

    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF168 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    HaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
    

    Expression

    Mammalian

    Promoter

    None

    Available Since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. AICSDP-078:G3BP1-mEGFP

    Plasmid

    #193920

    Purpose

    Homology arms and mEGFP-SGLRS for N-terminus tagging of human G3BP stress granule assembly factor 1

    Depositor

    Allen Institute for Cell Science

    Insert

    G3BP1 Homology Arms with mEGFP-SGLRS (G3BP1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    Dec. 22, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. LT2_14_GlyOx

    Plasmid

    #177711

    Purpose

    Plasmid for genome integration in X2 site expressing GlyRNA and OxPro (biosensors for glycolytic flux and oxidative stress)

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

    Inserts

    pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
    pTEFmut8-mCherry-tADH1
    pTRX2_5xUAS-ymYPET-tCYC1
    

    Use

    Used as donor dna in genome integration after lin…

    Expression

    Yeast

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. Halo-WIPI2 HRD

    Plasmid

    #207544

    Purpose

    Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous WIPI2 locus in human cells

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    HaloTag flanked by human WIPI2 locus sequences
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    Endogenous

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUCmini-UGCG-tdTomato

    Plasmid

    #225879

    Purpose

    Plasmid donor template for CRISPR/Cas9 mediated UGCG knockout by tdTomato insertion

    Depositor

    Yohei Sato

    Article

    Sato et al Biochem Biophys Res Commun. 2024 Sep 18;

    Insert

    tdTomato
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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