We narrowed to 18,336 results for: SHI

1. pBSKΔB-CBh-Cas9n-pA

   Plasmid

   #82617

   Purpose

   Expresses Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

   Insert

   SpCas9n
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO_eGFP-pScaC-MTS

   Plasmid

   #228827

   Purpose

   For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaC C-terminally fused to a mitochondrial targeting sequence in HeLa Flp-In cells

   Depositor

   Andrew Carter

   Article

   Manigrasso et al bioRxiv 2024.04.11.589026

   Insert

   ScaC
   

   Use

   Tags

   MTS and eGFP

   Expression

   Mammalian

   Mutation

   aa 33-223

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIVEX2.3-AC17-5c-frGFP

   Plasmid

   #217525

   Purpose

   AC17-5c riboswitch-regulated GFP reporter. The gene is inserted into downstream of T7 promoter.

   Depositor

   Keisuke Fukunaga

   Article

   Ishii et al Chem Commun (Camb). 2024 Jun 4;60(46):59

   Insert

   AC17-5c riboswitch-regulated folding reporter GFP
   

   Use

   In vitro transcription-translation

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIVEX2.3-Theo-3c-EGFP

   Plasmid

   #217526

   Purpose

   Theo-3c riboswitch-regulated GFP reporter. The gene is inserted into downstream of T7 promoter.

   Depositor

   Keisuke Fukunaga

   Article

   Ishii et al Chem Commun (Camb). 2024 Jun 4;60(46):59

   Insert

   Theo-3c riboswitch-regulated EGFP
   

   Use

   In vitro transcription-translation

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIRESneo-FLAG/HA-FL

   Plasmid

   #215839

   Purpose

   Expression of FLAG/HA tagged firefly luciferase (FL)

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

   Insert

   FLAG/HA tagged firefly luciferase (FL)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. psiCHECK1-CXCR4-MM4

   Plasmid

   #215846

   Purpose

   Expression of CXCR4 target with mismatch (MM) 4_

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

   Insert

   CXCR4 target with mismatch (MM) 4x
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. psiCHECK1-CXCR4

   Plasmid

   #215845

   Purpose

   Expression of CXCR4 target with perfect-match site

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

   Insert

   CXCR4 target with perfect-match site
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-FLAG/HA-p54

   Plasmid

   #215844

   Purpose

   Expression of FLAG/HA-p54

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

   Insert

   Human p54
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGWB602-po-mCerulean

   Plasmid

   #213861

   Purpose

   Binary vector for the expression of peroxisome transit peptide fused mCerulean in plants (for Agrobacterium-mediated genetic transformation and visualization of peroxisome.)

   Depositor

   Yutaka Kodama

   Article

   Ishikawa et al Plant Cell Rep. 2023 Mar;42(3):599-607.

   Insert

   Peroxisome transit peptide fused mCerulean
   

   Use

   Tags

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   Available since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Yeast_Target-AID2S

    Plasmid

    #188647

    Purpose

    Expressing Target-AID2S in yeasts

    Depositor

    Keiji Nishida

    Article

    Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

    Insert

    nCas9-AID2S-UGI
    

    Use

    Tags

    UGI

    Expression

    Yeast

    Mutation

    D10A for SpCas9

    Promoter

    pGal1

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLR5-U6-sgRNA-EF

    Plasmid

    #82635

    Purpose

    piggyBac vector for expressing modified sgRNA in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

    Type

    Empty backbone

    Use

    CRISPR; Piggybac vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pISH-Vmn2r76

    Plasmid

    #108084

    Purpose

    Preparation of RNA probe for ISH of mouse Vmn2r76 gene

    Depositor

    Peter Mombaerts

    Article

    Akiyoshi et al Eur J Neurosci. 2018 Apr;47(7):887-900.

    Insert

    Vmn2r76 (Vmn2r76 Mouse)
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmyc-GFP-TNRC6C-(928-1020)

    Plasmid

    #42026

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6C (TNRC6C Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    contain aa928-1020

    Promoter

    CMV

    Available since

    March 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-EML4-ALK variant 3a I1171N/D1203N

    Plasmid

    #183833

    Purpose

    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK I1171N/D1203N mutation in mammalian cells

    Depositor

    Aaron Hata

    Article

    Shiba-Ishii et al Nat Cancer. 2022 Jun 20. pii: 10.1038/s4

    Inserts

    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    

    Use

    Lentiviral

    Tags

    The fusion protein of EML4 exon 1-6a and ALK exon…

    Expression

    Mammalian

    Mutation

    I1171N/D1203N

    Promoter

    CMV

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-EF-FH-TAZ S89A-ires-blast

    Plasmid

    #52084

    Purpose

    Lentiviral expression vector for TAZ S89A mutant with N-terminal FLAG and His tags

    Depositor

    Yutaka Hata

    Article

    Yang et al Mol Cell Biol. 2014 Feb 18.

    Insert

    TAZ S89A (WWTR1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and His

    Expression

    Mammalian

    Mutation

    S89A

    Promoter

    EF1a

    Available since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-EF-FH-TAZ-ires-blast

    Plasmid

    #52083

    Purpose

    Lentiviral expression vector for TAZ with N-terminal FLAG and His tags

    Depositor

    Yutaka Hata

    Article

    Yang et al Mol Cell Biol. 2014 Feb 18.

    Insert

    TAZ (WWTR1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and His

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLe6Δ -hLP-mcs/(EGFP-IresPuro-hInt)

    Plasmid

    #64314

    Purpose

    chondrocyte-specific COL11A2 promoter/enhancer lentiviral reporter vector to select iChon cells

    Depositor

    Noriyuki Tsumaki

    Article

    Outani et al PLoS One. 2013 Oct 16;8(10):e77365. doi:

    Inserts

    COL11A2 promoter (COL11A2 Human)
    COL11A2 enhancer (COL11A2 Human)
    

    Use

    Lentiviral

    Tags

    EGFP and IRES-PURO

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAIDFA-EF1a-NmScarlet-mAID

    Plasmid

    #140615

    Purpose

    Expression of OsTIR1F74A and mScarlet-mAID protein in mammalian cells under the control of EF1a promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1 mutated F74A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAID-EF1a linearizing in pX330

    Plasmid

    #140610

    Purpose

    Crispr/Cas9 plasmid for linearization of pAID-EF1a

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    sgRNA for pAID-EF1a plasmid digestion
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRSETB/mStayGold

    Plasmid

    #212017

    Purpose

    mStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    mStayGold
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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