We narrowed to 5,085 results for: mcs

1. pAAV asyn S129D

   Plasmid

   #36069

   Depositor

   Patrick Aebischer, Hilal Lashuel

   Insert

   alpha-synuclein S129D (SNCA Human)
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   S129D

   Promoter

   CMV

   Available Since

   Sept. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pT2MP.EF-pHlu

   Plasmid

   #129304

   Purpose

   pH measurement, Tol2 transposon

   Depositor

   Yasushi Okada

   Article

   Yaginuma et al bioRxiv 2021.10.08.463131

   Insert

   pHluorin (from H. Imamura)
   

   Expression

   Mammalian

   Available Since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT2MPEF-TOMM-QUE37C

   Plasmid

   #191526

   Purpose

   ATP measurement, mitochondria outer membrane, Tol2 transposon

   Depositor

   Yasushi Okada

   Article

   Yaginuma et al bioRxiv 2021.10.08.463131

   Insert

   QUEEN-37C
   

   Expression

   Mammalian

   Available Since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCW57.1-GFP_mmu-miR-16-5p_sponge

   Plasmid

   #132624

   Purpose

   Expresses a TurboGFP transcript with microRNA sponge for mmu-miR-16-5p as 3'UTR

   Depositor

   Constance Ciaudo

   Insert

   stop codon, mmu-miR-16-5p sponge sequence and polyA signal
   

   Use

   Lentiviral; Doxycycline inducible

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT2MPEF-NLS-QUE37C

   Plasmid

   #129326

   Purpose

   ATP measurement, nucleus, Tol2 transposon

   Depositor

   Yasushi Okada

   Article

   Yaginuma et al bioRxiv 2021.10.08.463131

   Insert

   QUEEN-37C
   

   Expression

   Mammalian

   Available Since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

6. pJT260 Lenti pEF-dCas9-3XFLAG-VPR-T2A-tagBFP-P2A-Blast

   Plasmid

   #187336

   Purpose

   dCas9 effector fusion for manipulating transcription

   Depositor

   Michael Bassik, Lacra Bintu

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   dCas9-3XFLAG-VPR-T2A-tagBFP-P2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Promoter

   pEF1a

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAR015 Lenti pEF-dEnAsCas12a-NLS-NFZ-3XHA-T2A-BSD

   Plasmid

   #195544

   Purpose

   dCas12a effector fusion for manipulating transcription

   Depositor

   Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Lenti pEF-dEnAsCas12a-NLS-NFZ-3XHA-T2A-BSD
   

   Use

   CRISPR and Lentiviral

   Tags

   3XHA

   Expression

   Mammalian

   Promoter

   pEF1a

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIG-830_HA-GD2-28z_CAR_tNGFR_Retroviral

   Plasmid

   #207507

   Purpose

   This plasmid can be used to generate retrovirus.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   tNGFR, HA-GD2-28z_CAR (NGFR Human)
   

   Use

   Retroviral

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. dTAG-IRF8

   Plasmid

   #190620

   Purpose

   Lentiviral expression plasmid of human IRF8 fused with FKBP12G36V for dTAG-mediated degradation

   Depositor

   Junwei Shi

   Article

   Cao et al Mol Cell. 2021 Sep 2;81(17):3604-3622.e1

   Insert

   IRF8 (IRF8 Human)
   

   Use

   Lentiviral

   Tags

   FKBP12_G36V

   Expression

   Mammalian

   Mutation

   synonymous mutation for sgRNA resistance for sgIR…

   Available Since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-N1-PARP1

    Plasmid

    #211578

    Purpose

    CMV driven PARP1-eGFP expressing construct with IRES-Neomycin selection

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

    Insert

    PARP1 (PARP1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAR028 Lenti pEF-dEnAsCas12a-NLS-ZNF705F KRAB-3XHA-T2A-BSD

    Plasmid

    #195545

    Purpose

    dCas12a effector fusion for manipulating transcription

    Depositor

    Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti pEF-dEnAsCas12a-NLS-TopKRAB-3XHA-T2A-BSD
    

    Use

    CRISPR and Lentiviral

    Tags

    3XHA

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAR013 Lenti pEF-dEnAsCas12a-NLS-VP64-3XHA-T2A-BSD

    Plasmid

    #195547

    Purpose

    dCas12a effector fusion for manipulating transcription

    Depositor

    Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti pEF-dEnAsCas12a-NLS-VP64-3XHA-T2A-BSD
    

    Use

    CRISPR and Lentiviral

    Tags

    3XHA

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBABE-neomycin-PC-MYC

    Plasmid

    #184550

    Purpose

    Retroviral vector to express human PC with MYC tag

    Depositor

    Gerardo Ferbeyre

    Article

    Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

    Insert

    Pyruvate Carboxylase (PC Human)
    

    Use

    Retroviral

    Tags

    MYC

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-UbC-rtTA-Ngn2:2A:EGFP

    Plasmid

    #127288

    Purpose

    all-in-one inducible LV expression construct for Ngn2:2A:EGFP (aka. UNG; for direct neuronal conversion)

    Depositor

    Fred Gage

    Article

    Schafer et al Nat Neurosci. 2019 Feb;22(2):243-255. do

    Insert

    Ngn2:2A:Ascl1 (NEUROG2 Human)
    

    Use

    Lentiviral

    Tags

    Ngn2 and EGFP linked via 2A

    Expression

    Mammalian

    Available Since

    June 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5-FRT/TO-eGFP-msMMS22L

    Plasmid

    #221014

    Purpose

    mammalian expression vector of eGFP tagged mouse MMS22L

    Depositor

    Daniel Durocher

    Article

    O'Donnell et al Mol Cell. 2010 Nov 24;40(4):619-31. doi:

    Insert

    MMS22L (Mms22l Mouse)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    naturally siResistant to siMMS22L (human) aagacuu…

    Promoter

    CMV

    Available Since

    Sept. 5, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pCMV-HA-PD-L1

    Plasmid

    #121492

    Purpose

    Expression of human PD-L1 WT in mammalian cells

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    PD-L1 (CD274 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-N1-PARP1-E988K

    Plasmid

    #211580

    Purpose

    CMV driven PARP1-E988K -eGFP expressing construct with IRES-Neomycin selection

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

    Insert

    PARP1 (PARP1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Contains E988K mutation

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDH-Neo/Myc PD-L1

    Plasmid

    #121477

    Purpose

    expression of human Myc-PD-L1 in mamalian cell

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    CD274 (CD274 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-N1/PD-L1

    Plasmid

    #121478

    Purpose

    expression of human PD-L1 in mammalian cells

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    CD274 (CD274 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. CHFR-PBZ-eGFP

    Plasmid

    #211581

    Purpose

    Expressing CHFR PBZ domain fused to eGFP

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

    Insert

    CHFR PBZ domain (CHFR Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    PBZ domain of the protein

    Promoter

    CMV

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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