Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 3,333 results for: pEGFP

Clear keywords
Showing: 3301 - 3320 of 3333 results

  1. GFP-Cstem-Tac(5A)

    Plasmid
    #162497
    Purpose
    Expresses GFP tagged Tac mutant in mammalian cells. The five O-glycosylation sites in the stem region are mutated, and the stem region of CD8a is inserted between GFP and Tac(5A).
    Depositor
    Lei Lu
    Publication
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    CD8a stem region and Tac full length with mutated stem region (IL2RA, CD8A Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    The five threonine residues are mutated into alan…
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162497-plasmid-map-sequence-id-323484

    Enlarge

  2. GFP-Tac-STC(5A)

    Plasmid
    #162491
    Purpose
    Expresses non-O-glycosylated partial length Tac (stem region, TMD and cytosolic tail) with GFP tag in mammalian cells
    Depositor
    Lei Lu
    Publication
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    partial length Tac (stem region with mutations, TMD and cytosolic tail) (IL2RA Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Tac is in partial length with its stem region, TM…
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162491-plasmid-map-sequence-id-323469

    Enlarge

  4. SpyTag003(V114T, V116T)-Talin rod-mCherry

    Plasmid
    #210027
    Purpose
    Expression of SpyTag003(V114T, V116T)-Talin rod(434-2541)-mCherry in mammalian cells
    Depositor
    Vesa Hytönen
    Publication
    Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459
    Insert
    SpyTag003(V114T,V116T)-Talin1 rod domain(434-2541)-mCherry
    Expression
    Mammalian
    Mutation
    SpyTag003 V114T and V116T mutations to modify Spy…
    Promoter
    CMV
    Available since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    210027-plasmid-map-sequence-id-417751

    Enlarge

  6. GFP-Cstem(7A)-Tac(5A)

    Plasmid
    #162499
    Purpose
    Expresses GFP tagged Tac mutant in mammalian cells. The five O-glycosylation sites in the stem region are mutated, and the stem region of CD8a with mutations is inserted between GFP and Tac(5A).
    Depositor
    Lei Lu
    Publication
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    CD8a stem region with mutations and Tac full length with mutated stem region (IL2RA, CD8A Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    The five theronine and two serine residues are mu…
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162499-plasmid-map-sequence-id-323491

    Enlarge

  9. pRSET a sfMatryoshCaMP6s-T78H

    Plasmid
    #100021
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference LSSmOrange nested within the reporting superfolder cpGFP-T78H
    Depositor
    Wolf Frommer
    Publication
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    sfMatryoshCaMP6s
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    cpEGFP replaced with superfolder cpGFP and T78H m…
    Promoter
    T7
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100021-plasmid-map-sequence-id-194294

    Enlarge

  11. GFP-Tollip M240A,F241A

    Plasmid
    #178051
    Purpose
    GFP fused to human Tollip cDNA carrying mutations in the CUE domain. Localisation and expression in mammalian cells.
    Depositor
    David Tumbarello
    Publication
    Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:
    Insert
    Toll-Interacting Protein (TOLLIP Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Changed Methionine 240 to Alanine and changed Phe…
    Promoter
    CMV
    Available since
    Dec. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    178051-plasmid-map-sequence-id-350116

    Enlarge

  14. pRSET a sfMatryoshCaMP6s

    Plasmid
    #100020
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting superfolder cpGFP.
    Depositor
    Wolf Frommer
    Publication
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    sfMatryoshCaMP6s
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    cpEGFP replaced with superfolder cpGFP
    Promoter
    T7
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100020-plasmid-map-sequence-id-194297

    Enlarge

Showing: 3301 - 3320 of 3333 results