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pRG711_lexO-AscI_LexA-TF_HIS3MX

Plasmid

#154820

Purpose

Inducible expression of AscI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

AscI endonuclease
LexA-ER-B112

Tags

FLAG and NLS

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pET151-Vta1(280-330)

Plasmid

#87738

Purpose

Bacterial expression of the VSL domain of S. cerevisiae Vta1

Depositor

Chris Hill

Article

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein Vta1 (VTA1 Budding Yeast)

Tags

6xHis-V5-TEV

Expression

Bacterial

Mutation

deleted amino acids 1-279

Promoter

T7

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pICH47742:PtFCP:Cas9YFP

Plasmid

#104894

Purpose

Cas9, with FCP promoter/terminator, Golden Gate Assembly, for Phaeodactylum transformation

Depositor

Thomas Mock

Article

Graff van Creveld et al Front Microbiol. 2021 Sep 8;12:688199. d

Insert

Cas9

Use

CRISPR; ; golden gate assembly

Tags

YFP

Available Since

July 11, 2018

Availability

Academic Institutions and Nonprofits only

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pILGFPQ3

Plasmid

#83558

Purpose

Used to evaluate the expression output of GAL2 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

Insert

GAL2 promoter-yEGFP

Expression

Yeast

Promoter

GAL2

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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Dom34+GST

Plasmid

#19099

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Protein DOM34 (DOM34 Budding Yeast)

Tags

C tag, GST, and N tag

Expression

Bacterial

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
Dom34(1-138)

Plasmid

#19101

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Protein DOM34 (DOM34 Budding Yeast)

Tags

C tag

Expression

Bacterial

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
pGS1044

Plasmid

#63797

Purpose

Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal 3xFLAG tag and a LEU2 selection marker

Depositor

Monika Golas, Bjoern Sander

Article

Lin et al Biochim Biophys Acta. 2015 Mar;1854(3):1

Insert

3xFLAG followed by KlLEU2

Use

E. coli cloning vector

Tags

3xFLAG

Promoter

none

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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Dom34 K82A

Plasmid

#19112

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Protein DOM34 (DOM34 Budding Yeast)

Tags

C tag

Expression

Bacterial

Mutation

K82A

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
pJV457

Plasmid

#180080

Purpose

M.BbrUI expression construct to improve transformation in B. breve UCC2003

Depositor

Chase Beisel

Article

Vento et al Mol Cell. 2024 Jul 25;84(14):2785-2796.e

Insert

M.BbrUI

Expression

Bacterial

Promoter

P70a

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pJV461

Plasmid

#180081

Purpose

M.BbrUIII expression construct to improve transformation in B. breve UCC2003

Depositor

Chase Beisel

Article

Vento et al Mol Cell. 2024 Jul 25;84(14):2785-2796.e

Insert

M.BbrUIII

Expression

Bacterial

Promoter

P70a

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2309_pGEEC524_pShip-EFS-ZF10-NS3-p65-bGH

Plasmid

#239722

Purpose

Plasmid expressing a grazoprevir-inducible transcriptional activator

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

ZF10-NS3-p65

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pILFPZ1A9

Plasmid

#223835

Purpose

Expressing a cytosolic pH sensor (mTagBFP2-mNeonGreen) in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Evans et al ACS Synth Biol. 2025 Sep 19;14(9):3804-3

Insert

pTEF1>mTagBFP>mNeonGreen>

Expression

Yeast

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pILFPQ13A7

Plasmid

#223837

Purpose

Expressing mScarletI3 under the control of the TEF1 promoter in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Evans et al ACS Synth Biol. 2025 Sep 19;14(9):3804-3

Insert

pTEF1>mScarletI3>

Expression

Yeast

Promoter

TEF1

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pILFPQ13B7

Plasmid

#223838

Purpose

Expressing StayGold under the control of the TEF1 promtoer in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Evans et al ACS Synth Biol. 2025 Sep 19;14(9):3804-3

Insert

pTEF1>StayGold>

Expression

Yeast

Promoter

TEF1

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pILFPmTagBFP2FrFAST

Plasmid

#223839

Purpose

Expressing mTagBFP2-FrFAST under the control of the TEF1 promtoer in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Evans et al ACS Synth Biol. 2025 Sep 19;14(9):3804-3

Insert

pTEF1>mTagBFP2>FrFAST

Expression

Yeast

Promoter

TEF1

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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BtA2-3A

Plasmid

#238984

Purpose

In vitro transcription of mutant bovine arrestin-2 long isoform

Depositor

Vsevolod Gurevich

Article

Celver et al J Biol Chem. 2002 Mar 15;277(11):9043-8.

Insert

arrestin-2 long (ARRB2 Bovine)

Use

In vitro transcription

Mutation

I386A,V387A,F388A

Promoter

SP6

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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BtA1N

Plasmid

#238979

Purpose

In vitro transcription of bovine arrestin-1

Depositor

Vsevolod Gurevich

Article

Vishnivetskiy et al J Biol Chem. 2004 Jan 9;279(2):1262-8. d

Insert

arrestin-1 (SAG Bovine)

Use

In vitro transcription

Mutation

silent

Promoter

SP6

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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BtA2N

Plasmid

#238980

Purpose

In vitro transcription of bovine arrestin-2 long isoform

Depositor

Vsevolod Gurevich

Article

Vishnivetskiy et al J Biol Chem. 2004 Jan 9;279(2):1262-8. d

Insert

arrestin-2 long (ARRB2 Bovine)

Use

In vitro transcription

Mutation

silent

Promoter

SP6

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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BtARR1 (1-378)

Plasmid

#236264

Purpose

Bacterial expression of bovine arrestin-1 (1-378) E379Stop

Depositor

Vsevolod Gurevich

Article

Vishnivetskiy et al Curr Protoc. 2023 Sep;3(9):e832. doi: 10

Insert

arrestin-1 (SAG Bovine)

Expression

Bacterial

Mutation

(1-378) E379Stop, inserted GGT codon after starti…

Promoter

lac

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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BtARR1

Plasmid

#236262

Purpose

Bacterial expression of bovine arrestin-1 with inserted GGT codon after starting ATG

Depositor

Vsevolod Gurevich

Article

Vishnivetskiy et al Curr Protoc. 2023 Sep;3(9):e832. doi: 10

Insert

arrestin-1 (SAG Bovine)

Expression

Bacterial

Mutation

inserted GGT codon after starting ATG

Promoter

lac

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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