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pLV_PGK_3xHA-LTB4R(T308A)

Plasmid

#208092

Purpose

Mammalian expression of the human LTB4 receptor LTB4R with the T308A mutation with an N-terminal 3x HA tag.

Depositor

Sean R Collins

Article

Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

Insert

3xHA-LTB4R(T308A) (LTB4R Human)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

T308A

Promoter

PGK

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLV_PGK_3xHA-LTB4R(S310A)

Plasmid

#208093

Purpose

Mammalian expression of the human LTB4 receptor LTB4R with the S310A mutation with an N-terminal 3x HA tag.

Depositor

Sean R Collins

Article

Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

Insert

3xHA-LTB4R(S310A) (LTB4R Human)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

S310A

Promoter

PGK

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLV_PGK_3xHA-LTB4R

Plasmid

#208091

Purpose

Mammalian expression of the wild-type human LTB4 receptor LTB4R with an N-terminal 3x HA tag.

Depositor

Sean R Collins

Article

Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

Insert

3xHA-LTB4R (LTB4R Human)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

none

Promoter

PGK

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pROF018

Plasmid

#155325

Purpose

Constitutive expression of KRAB-EL222 in plants

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

PCaMV35S-NLS-KRAB-EL222-Tnos

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pROF050

Plasmid

#155327

Purpose

Constitutive expression of BRD-EL222 in plants

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

PCaMV35S-BRD-NLS-EL222-Tnos

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pROF366

Plasmid

#155332

Purpose

Expression of FLuc under the control of AtAP1 promoter

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

PAtAP1-FLuc-TSV40

Use

Luciferase and Synthetic Biology

Expression

Plant

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.0-TERT_-100_-79

Plasmid

#193785

Purpose

luciferase reporter for duplicated TERT promoter

Depositor

Joseph Costello

Article

Barger et al Nat Commun. 2022 Sep 16;13(1):5430. doi:

Insert

TERT promoter (TERT Human)

Use

Luciferase

Expression

Mammalian

Promoter

TERT

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-SNRPA_sgRNA1

Plasmid

#201622

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

SNRPA (SNRPA Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-SNRPA_sgRNA2

Plasmid

#201623

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

SNRPA (SNRPA Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-NDUFB5_sgRNA1

Plasmid

#201606

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

NDUFB5 (NDUFB5 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-NDUFB5_sgRNA2

Plasmid

#201607

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

NDUFB5 (NDUFB5 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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KTK_139

Plasmid

#180565

Purpose

Spacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Spacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2

Expression

Bacterial

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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AttB_ACE2[R.ferrum]-2A-TMPRSS2_IRES_iRFP670-H2A-P2A-PuroR

Plasmid

#190078

Purpose

express Rhinolophus ferrumequinum ACE2 and human TMPRSS2 with selectable markers iRFP670 and puromycin resistance gene

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

ACE2 (TMPRSS2 Rhinolophus ferrumequinum)

Expression

Mammalian

Promoter

none

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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AttB_ACE2[HA]_IRES_iRFP670-H2A-P2A-PuroR

Plasmid

#190080

Purpose

express cytoplasmically HA-tagged human ACE2 with selectable markers iRFP670 and puromycin resistance gene

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

ACE2 (ACE2 Human)

Tags

HA

Expression

Mammalian

Promoter

none

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_113

Plasmid

#180540

Purpose

Entry vector containing Signal peptide region of CsgA including SecB-N22 sequenced

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Signal peptide region of CsgA including SecB-N22 sequenced

Expression

Bacterial

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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p8322 pDONR ATG-YAP1 S94A-Stop

Plasmid

#184526

Purpose

Subcloning YAP1 S94A

Depositor

Elizabeth White

Article

Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

Insert

YAP1 S94A (YAP1 Human)

Use

Unspecified

Mutation

S94A

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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p8321 pDONR ATG-YAP1 S127A-Stop

Plasmid

#184525

Purpose

Subcloning YAP1 S127A

Depositor

Elizabeth White

Article

Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

Insert

YAP1 S127A (YAP1 Human)

Use

Unspecified

Mutation

S127A

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_095

Plasmid

#180524

Purpose

dCas9 KTK compatible plasmid. Contains dCas9 and lacZ dropout region with flanking D1.1 overhangs for insertion of gRNA expression assembly

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_324

Plasmid

#180566

Purpose

Spacer sequence in 2.2b format to allow for level 3 cloning with D2.2a

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Spacer sequence in 2.2b format to allow for level 3 cloning with D2.2a

Expression

Bacterial

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV(Δ3)-squirrel-monkey-full-length-CHMP3-HA

Plasmid

#154169

Purpose

expresses squirrel monkey CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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