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Addgene

We narrowed to 37,102 results for: RAN-1

Showing: 8301 - 8350 of 37102 results
  1. pCL21-decoder

    Plasmid
    #159448
    Purpose
    Insert: Pact1-lexA-ER-B112-phosphodegron-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is degraded upon addition of alpha factor. Backbone: pRG201 (addGene). Marker: Met15.
    Depositor
    Insert
    Pact1-lexA-ER-B112-phosphodegron-tCyc1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    act1
    Available since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
  2. pCL38-decoder

    Plasmid
    #159452
    Purpose
    Insert: Pfus1tet-lexA-ER-B112-tCyc1.The transcription factor LexA-ER-B112 (see addgene FRP880) is inducible by alpha factor, and repressible by TetR. Backbone: pRG235 (addgene). Marker: Leu2
    Depositor
    Insert
    Pfus1tet-LexA-ER-B112-tCyc1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Pfus1tet
    Available since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
  3. pDmelOR-mRho.V5.mER.hOr47a

    Plasmid
    #126478
    Purpose
    Human codon-optimized D. melanogaster Orco (hOrco) and Or47a (hOr47a). Or47a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells
    Depositor
    Inserts
    Or47a (Or47a Fly)
    Orco (Orco Fly)
    Use
    Tags
    H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…
    Expression
    Mammalian
    Mutation
    codon optimization for H. sapiens and codon optim…
    Promoter
    CMV
    Available since
    June 13, 2019
    Availability
    Academic Institutions and Nonprofits only
  4. pCMK83

    Plasmid
    #226793
    Purpose
    Integrates slr0214 (HIP1 methyltransferase) into gidB-atpI locus of E. coli DH10B
    Depositor
    Insert
    slr0214 (slr0214 Synechocystis sp. PCC 6803)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Tetracycline resistance cassette promoter (consti…
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
  5. pSP569

    Plasmid
    #139499
    Purpose
    Repair DNA plasmid to insert PP7 stem loops in the endogenous STL1 locus after double strand break in S. cerevisiae
    Depositor
    Insert
    pSTL1 24xPP7sl STL1 100 - 600
    Use
    Tags
    Expression
    Yeast
    Mutation
    WT
    Promoter
    pSTL1
    Available since
    June 12, 2020
    Availability
    Academic Institutions and Nonprofits only
  6. pMAZ.1.0-gDNA

    Plasmid
    #112455
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor MAZ
    Depositor
    Insert
    MAZ (MAZ Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
  7. pET3d-SF-DCTN1-TS

    Plasmid
    #169375
    Purpose
    E. coli expression plasmid for human DCTN1 (longest transcript variant p150glued/isoform 1), with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.
    Depositor
    Insert
    dynactin subunit 1 isoform 1 (DCTN1 Human)
    Use
    Tags
    6xHis Tag, SplitFlAsH Tag, and TwinStrep Tag
    Expression
    Bacterial
    Mutation
    Point mutations: A139T, I304T, Q322R, T516A, E577…
    Promoter
    Available since
    May 27, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. pAAV-CAG-DIO-ChR2(H134R)-eYFP

    Plasmid
    #127090
    Purpose
    Cre-dependent AAV expression of humanized ChR2 (with H134R mutation) fused to eYFP for optogenetic activation
    Depositor
    Has Service
    AAV PHP.eB
    Insert
    Channelrhodopsin-2
    Use
    AAV
    Tags
    eYFP (C terminal on insert)
    Expression
    Mammalian
    Mutation
    Humanized ChR2 gene with histidine 134 changed to…
    Promoter
    CAG
    Available since
    June 13, 2019
    Availability
    Academic Institutions and Nonprofits only
  9. pTRE3G-FLAG-human cGAS N-T2A-deltaN-HA

    Plasmid
    #186886
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Insert
    Human cGAS with T2A ribosome skipping sequences between N-terminus (1-159 a.a.) and C-terminus (160-522 a.a.) (Adamts1 Human)
    Use
    Retroviral
    Tags
    FLAG and HA
    Expression
    Mutation
    Insertion of T2A ribosome skipping site
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 8301 - 8350 of 37102 results