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pRVV700

Plasmid

#119017

Purpose

Donor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

Insert

TP901-1 attP sites

Use

CRISPR; Donor vector for creation of platforms fo…

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pRVV609

Plasmid

#119014

Purpose

Donor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

Insert

TP901-1 attP sites

Use

CRISPR; Donor vector for creation of platforms fo…

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pRVV688

Plasmid

#119015

Purpose

Donor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

Insert

TP901-1 attP sites

Use

CRISPR; Donor vector for creation of platforms fo…

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRVV689

Plasmid

#119016

Purpose

Donor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

Insert

TP901-1 attP sites

Use

CRISPR; Donor vector for creation of platforms fo…

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAW205

Plasmid

#113245

Purpose

E. coli expression vector for IHFa D14A + IHFb

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

IHFa D14A (ihfA E. coli)
IHFb

Expression

Bacterial

Mutation

D14A

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pPS3911

Plasmid

#113330

Purpose

control for Hermes transposition; TIR-flanked LEU2, transposase absent

Depositor

Suzanne Sandmeyer

Article

Patterson et al Metab Eng. 2018 May 22;48:184-196. doi:

Insert

beta-isopropyl malate dehydrogenase

Expression

Yeast

Available Since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAW181

Plasmid

#113127

Purpose

E. coli expression vector for His-MBP-E. coli Cas1 R138A

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Insert

Cas1

Tags

6xHis-MBP

Expression

Bacterial

Mutation

R138A

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAW210

Plasmid

#113189

Purpose

E. coli expression vector for untagged E. coli Cas1 H208A + Cas2 + CRISPR array

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

H208A

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pVKG1-BSD

Plasmid

#108680

Purpose

Blasticidin resistant cassette for knock-in by VIKING method

Depositor

Shigeo Sugano

Article

Sawatsubashi et al Sci Rep. 2018 Jan 12;8(1):593. doi: 10.1

Insert

SV40pro-BSD-polyA

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pWT029g

Plasmid

#96860

Purpose

guanine-agRNA (GFP activation, 18 nt blocker with bulges)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

guanine-agRNA (GFP activation, 18 nt blocker with bulges)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWT029h

Plasmid

#96861

Purpose

(d)guanine-agRNA (GFP activation,18 nt blocker with bulges)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

(d)guanine-agRNA (GFP activation,18 nt blocker with bulges)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWT049d

Plasmid

#96862

Purpose

guanine-agRNA (19 nt blocker with bulges, RFP activation)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

guanine-agRNA (19 nt blocker with bulges, RFP activation)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pWT049e

Plasmid

#96863

Purpose

(d)guanine-agRNA (19 nt blocker with bulges, RFP activation)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

(d)guanine-agRNA (19 nt blocker with bulges, RFP activation)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWT016h

Plasmid

#96854

Purpose

HHR-bsgRNA-GFP (17 nt blocker)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

HHR-bsgRNA-GFP (17 nt blocker)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pWT016d

Plasmid

#96855

Purpose

dHHR-bsgRNA-GFP (17 nt blocker)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

dHHR-bsgRNA-GFP (17 nt blocker)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWT016f

Plasmid

#96850

Purpose

HHR-bsgRNA-GFP (10 nt blocker)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

HHR-bsgRNA-GFP (10 nt blocker)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWT016e

Plasmid

#96851

Purpose

dHHR-bsgRNA-GFP (10 nt blocker)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

dHHR-bsgRNA-GFP (10 nt blocker)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWT016a

Plasmid

#96852

Purpose

HHR-bsgRNA-GFP (13 nt blocker)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

HHR-bsgRNA-GFP (13 nt blocker)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pWT016b

Plasmid

#96853

Purpose

dHHR-bsgRNA-GFP (13 nt blocker)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

dHHR-bsgRNA-GFP (13 nt blocker)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pAP757-1

Plasmid

#99493

Purpose

6XHis::TEV::3XFlag::linker::Myc

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

6XHis::TEV::3XFlag::linker::Myc

Expression

Bacterial

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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