Skip to main content
Addgene
Showing: 5011 - 5040 of 8427 results

  3. pSPDEF-donor

    Plasmid
    #113828
    Purpose
    CRISPR donor plasmid to tag human transcription factor SPDEF with GFP
    Depositor
    Kevin White
    Insert
    SPDEF homology arms flanking EGFP-IRES-Neo cassette (SPDEF Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    113828-plasmid-map-sequence-id-230205

    Enlarge

  4. pTFCP2-donor

    Plasmid
    #112308
    Purpose
    CRISPR donor plasmid to tag human transcription factor TFCP2 with GFP
    Depositor
    Kevin White
    Insert
    TFCP2 homology arms flanking EGFP-IRES-Neo cassette (TFCP2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    112308-plasmid-map-sequence-id-225589

    Enlarge

  5. pZC10 (pFA6a-PSc-6xGly-V5-His3MX)

    Plasmid
    #44067
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, His3…
    Expression
    Mutation
    Promoter
    Available since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44067-plasmid-map-sequence-id-61693

    Enlarge

  6. pTF266 (pFA6a-PSc-6xGly-3xFlag-His3MX)

    Plasmid
    #44065
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    3xFlag, 6xGly linker, ADH1 transcription terminat…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44065-plasmid-map-sequence-id-61669

    Enlarge

  7. pOVOL1-donor

    Plasmid
    #113826
    Purpose
    CRISPR donor plasmid to tag human transcription factor OVOL1 with GFP
    Depositor
    Kevin White
    Insert
    OVOL1 homology arms flanking EGFP-IRES-Neo cassette (OVOL1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs496388
    Promoter
    Available since
    Dec. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    113826-plasmid-map-sequence-id-230200

    Enlarge

  8. pZC14 (pFA6a-PSc-6xGly-V5-HphMX)

    Plasmid
    #44079
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, HphM…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44079-plasmid-map-sequence-id-61697

    Enlarge

  9. pCB30

    Plasmid
    #111910
    Purpose
    Expresses gRNA to target URA3 knockout locus and has KanMX marker
    Depositor
    Yi Tang
    Insert
    gRNA cassette targeting URA3 knockout locus
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    SNR52p
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    111910-plasmid-map-sequence-id-218310

    Enlarge

  10. pRAV24

    Plasmid
    #67716
    Purpose
    Parental plasmid for transcription of RNAs with a GlmS/M-domain 3'-tag for native purification
    Depositor
    Robert Batey, Jeffrey Kieft
    Article
    Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju
    Insert
    Transcription cassette for 3'-tagged RNAs
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 RNA polymerase
    Available since
    Sept. 8, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. pTF274 (pFA6a-PSc-6xGly-3xFlag-HphMX)

    Plasmid
    #44077
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    3xFlag, 6xGly linker, ADH1 transcription terminat…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44077-plasmid-map-sequence-id-61678

    Enlarge

  13. pZC12 (pFA6a-PSc-6xGly-V5-KanMX)

    Plasmid
    #44091
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, KanM…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44091-plasmid-map-sequence-id-61695

    Enlarge

  14. pQdCas9-sggfp

    Plasmid
    #236184
    Purpose
    The plasmid pQdCas9-sggfp expresses the dCas9 endonuclease and the sgRNA targeting the gfp gene.
    Depositor
    Radhakrishnan Mahadevan
    Insert
    Quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas9, and sgRNA for GFP gene
    Use
    Tags
    Expression
    Mutation
    Promoter
    Quorum sensing promoter
    Available since
    April 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    236184-plasmid-map-sequence-id-470109

    Enlarge

  15. pQdCas12a.sggfp(C)

    Plasmid
    #236042
    Purpose
    The plasmid pQdCas12a.sggfp(C) expresses the dCas12a endonuclease and the sgRNA (design C) targeting the gfp gene.
    Depositor
    Radhakrishnan Mahadevan
    Insert
    quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (C) of gfp gene
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    quorum sensing promoter
    Available since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236042-plasmid-map-sequence-id-469925

    Enlarge

  16. pAAV-U6-DR30(SapI) 0.5 Syn-intron-CasRx-pA

    Plasmid
    #233038
    Purpose
    To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx from a 0.5 bp synapsin promoter
    Depositor
    Jonathan Ploski
    Insert
    RfxCas13d-compatible gRNA expression cassette and CasRx
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    233038-plasmid-map-sequence-id-463774

    Enlarge

  17. AA566

    Plasmid
    #231115
    Purpose
    Fragmid fragment: (guide cassette) ABE activity-based selection cloning
    Depositor
    David Root, John Doench
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    231115-plasmid-map-sequence-id-458370

    Enlarge

  18. AA568

    Plasmid
    #231117
    Purpose
    Fragmid fragment: (guide cassette) CBE activity-based selection cloning
    Depositor
    David Root, John Doench
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    231117-plasmid-map-sequence-id-458363

    Enlarge

  19. GBH-2aG4-gcryBFP.1

    Plasmid
    #188013
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted 2aGal4VP16 protein trap; secondary marker gamma crystallin BFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; gamma crystallin promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188013-plasmid-map-sequence-id-381108

    Enlarge

  20. GBH-2aG4-gcryBFP.2

    Plasmid
    #188014
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted 2aGal4VP16 protein trap; secondary marker gamma crystallin BFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; gamma crystallin promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188014-plasmid-map-sequence-id-381045

    Enlarge

  21. GBH-2aG4-gcryGFP.1

    Plasmid
    #188016
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted 2aGal4VP16 protein trap; secondary marker gamma crystallin GFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; gamma crystallin promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188016-plasmid-map-sequence-id-381139

    Enlarge

  22. GBH-2aG4-gcryGFP.2

    Plasmid
    #188017
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted 2aGal4VP16 protein trap; secondary marker gamma crystallin GFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; gamma crystallin promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188017-plasmid-map-sequence-id-381140

    Enlarge

  23. GBH-2aG4-gcryGFP.3

    Plasmid
    #188018
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted 2aGal4VP16 protein trap; secondary marker gamma crystallin GFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; gamma crystallin promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188018-plasmid-map-sequence-id-381141

    Enlarge

  25. FNF-CAG-L-3xSV40pA-L-tdTomato shuttle vector

    Plasmid
    #182346
    Purpose
    PacI-flanked CAG-LSL expression cassette with a FNF for positive selection
    Depositor
    Mario Capecchi
    Insert
    tdTomato
    Use
    Mouse Targeting; Flp / frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182346-plasmid-map-sequence-id-358221

    Enlarge

  26. cTRE-LSL-GFP-IRES-Cas9-sgPtenX1.1

    Plasmid
    #135669
    Purpose
    Introduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus Pten-targeting sgRNA into (ES) cells by recombination-mediated cassette exchange
    Depositor
    Florian Karreth
    Insert
    sgPten
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135669-plasmid-map-sequence-id-325841

    Enlarge

  27. pZNF174-donor

    Plasmid
    #112301
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF174 with GFP
    Depositor
    Kevin White
    Insert
    ZNF174 homology arms flanking EGFP-IRES-Neo cassette (ZNF174 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs37813, rs39729
    Promoter
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112301-plasmid-map-sequence-id-228413

    Enlarge

  28. pZNF257-donor

    Plasmid
    #112294
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF257 with GFP
    Depositor
    Kevin White
    Insert
    ZNF257 homology arms flanking EGFP-IRES-Neo cassette (ZNF257 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112294-plasmid-map-sequence-id-225583

    Enlarge

  29. pZNF586-donor

    Plasmid
    #112324
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF586 with GFP
    Depositor
    Kevin White
    Insert
    ZNF586 homology arms flanking EGFP-IRES-Neo cassette (ZNF586 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:19:57779607:T>A
    Promoter
    Available since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    112324-plasmid-map-sequence-id-228538

    Enlarge

  30. pZNF134-donor

    Plasmid
    #112320
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF134 with GFP
    Depositor
    Kevin White
    Insert
    ZNF134 homology arms flanking EGFP-IRES-Neo cassette (ZNF134 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:19:57621700:C>T
    Promoter
    Available since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    112320-plasmid-map-sequence-id-228529

    Enlarge

Showing: 5011 - 5040 of 8427 results