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pEGFP-Akt1(D323A/D325A)

Plasmid

#86630

Purpose

Expresses eGFP-tagged human Akt1(D323A/D325A) mutant

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1(D323A/D325A) (AKT1 Human)

Expression

Mammalian

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2 xRor2-mCherry

Plasmid

#67162

Purpose

mammalian expression / in vitro transcription

Depositor

Alexandra Schambony

Article

Feike et al Biochem Biophys Res Commun. 2010 Oct 1;4

Insert

Tyrosine-protein kinase transmembrane receptor ROR2 (ror2 Frog)

Use

In vitro transcription

Tags

mCherry

Expression

Mammalian

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only
pCMB-ACTLg

Plasmid

#61195

Purpose

Fluorescent reporter for F-actin LifeAct-eGFP expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

LifeAct-eGFP

Tags

eGFP

Expression

Plant

Promoter

MtBCP1

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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NET50 pmRFP-N2 (489)

Plasmid

#61983

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

Insert

NET50 (DHRS7 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pDEST15 hSlp4-a

Plasmid

#40046

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a (SYTL4 Human)

Tags

GST

Expression

Bacterial

Promoter

T7

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
TAL2457

Plasmid

#36846

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

VHL-TALEN_Right (Vhl Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

July 31, 2012

Availability

Academic Institutions and Nonprofits only
TAL2456

Plasmid

#36845

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

VHL-TALEN_Left (Vhl Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2208

Plasmid

#36671

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

HOXA13-TALEN_Left (HOXA13 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2200

Plasmid

#36663

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

AXIN2-TALEN_Left (AXIN2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2201

Plasmid

#36664

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

AXIN2-TALEN_Right (AXIN2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pMA5.1 (mouse integrin alpha 5 probe)

Plasmid

#14097

Depositor

Richard Hynes

Article

Yang et al Development. 1993 Dec . 119(4):1093-105.

Insert

Mouse Alpha 5 Integrin (Itga5 Mouse)

Expression

Bacterial

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-Xl-VSP1 R152Q GFP

Plasmid

#51884

Purpose

expression of Xl-VSP1 mutant fused to GFP in Xenopus oocytes or mammalian cells

Depositor

Laurinda Jaffe

Article

Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

Insert

VSP1 R152Q (tpte2 Frog)

Use

Xenopus oocyte expression

Tags

EGFP

Expression

Mammalian

Mutation

R152Q: near the extracellular side of the fourth …

Promoter

CMV

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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pME-zFUCCI (JDW 1464)

Plasmid

#242558

Purpose

Gateway middle entry clone containing zebrafish FUCCI reporter; For visualizing cell cycle state.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCerulean-zGeminin(1-100) P2A mCherry-zCdt1(1-190)

Use

Gateway Cloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pME-EGFP-P2A-iCre (JDW 921)

Plasmid

#224519

Purpose

A Gateway compatible middle entry clone containing EGFP P2A iCre (codon optimized cre)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EGFP-P2A-iCre

Use

Cre/Lox

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 p37

Plasmid

#113500

Purpose

Bacterial expression of GST-tagged p37

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p37 (Ubxn2b Mouse)

Tags

GST-PreScission protease site

Expression

Bacterial

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pT2K-LRL-IRES-Luc2

Plasmid

#109230

Purpose

For Tol2 transposon-mediated stable expression of LRL and IRES-luciferase

Depositor

Martine Roussel

Article

Kawauchi et al Oncogene. 2017 Sep 14;36(37):5231-5242.

Insert

LRL and Luc2

Expression

Mammalian

Promoter

CAGGS

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a- mCFP-kanMX6

Plasmid

#105145

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

mCFP-kanMX6

Tags

mCFP

Expression

Bacterial and Yeast

Mutation

A206K

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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PNCA-GFP-dYY1

Plasmid

#67791

Purpose

Use together with pCMV-intron and VSV-G to package MMLV reporter virus

Depositor

Stephen Goff

Article

Schlesinger et al Cell Rep. 2013 Jul 11;4(1):50-8. doi: 10

Insert

MMLV-LTR-GFP

Mutation

Deletion of LTR U3 YY1 binding domain

Available Since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only
TAL2375

Plasmid

#36786

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

SETDB1-TALEN_Right (Setdb1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2382

Plasmid

#36791

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

AKT2-TALEN_Left (Akt2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only