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Beta-Lactamase

Plasmid

#62729

Purpose

Expresses Beta-Lactamase protein in bacterial cells

Depositor

Niels Geijsen

Publication

D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

Insert

Beta-Lactamase

Tags

His tag

Expression

Bacterial

Mutation

Deletion of periplasmic signal domain

Promoter

T7

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJZC34

Plasmid

#62331

Purpose

sgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Publication

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 2x MS2(wt+f6) binding module
MCP-VP64

Use

Lentiviral

Tags

VP64

Expression

Mammalian

Mutation

Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

Promoter

CMV and U6

Available since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMXs-Lhx3

Plasmid

#32927

Depositor

Kevin Eggan

Publication

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

Lhx3 (Lhx3 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pcdna6.2- myc ULK1 wt

Plasmid

#27629

Depositor

Reuben Shaw

Publication

Egan et al Science. 2011 Jan 28;331(6016):456-61. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mouse myc ULK1 (Myc Mouse)

Tags

myc

Expression

Mammalian

Mutation

Initial M from ULK1 removed. Silent mutation at A…

Available since

Feb. 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
pDONR223-LYN

Plasmid

#23905

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

LYN (LYN Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pDONR223-PRKAA2

Plasmid

#23671

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKAA2 (PRKAA2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Flag-HA-JOSD1

Plasmid

#22547

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

JOSD1 (JOSD1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLenti X2 Neo DEST (w18-1)

Plasmid

#17390

Purpose

3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, Neo

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning, Lentiviral, and RNAi

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pB1H2w5-Prd

Plasmid

#18039

Depositor

Scot Wolfe

Publication

Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

Insert

Paired (prd Fly)

Expression

Bacterial

Mutation

Paired is simply used as an insert between Kpn1 a…

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUC8 mouse IFN-gamma

Plasmid

#10955

Depositor

Howard Young

Insert

interferon-gamma (Ifng Mouse)

Use

Cloning vector

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pmyc-MOV10

Plasmid

#10977

Depositor

Thomas Tuschl

Publication

Meister et al Curr Biol. 2005 Dec 6;15(23):2149-55. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MOV10 (MOV10 Human)

Tags

myc

Expression

Mammalian

Available since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pFLAG/HA-TNRC6B

Plasmid

#10979

Depositor

Thomas Tuschl

Publication

Meister et al Curr Biol. 2005 Dec 6;15(23):2149-55. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TNRC6B (TNRC6B Human)

Tags

FLAG and HA

Expression

Mammalian

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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[#GS5-RV] MitoTRACER-Donor

Plasmid

#233505

Purpose

Retroviral construct of the MitoTRACER genetic reporter to be expressed in the donor cells

Depositor

Simon Grelet

Publication

Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

Insert

MitoTRACER-Donor

Use

Retroviral and Synthetic Biology

Tags

GFP11 and HA Tag

Expression

Mammalian

Available since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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V5-GFP-TurboID-KDEL_pLX208

Plasmid

#202020

Purpose

expresses GFP-TurboID in the mammalian ER lumen, lentiviral vector

Depositor

Alice Ting

Publication

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

V5-TurboID-KDEL

Use

Lentiviral

Tags

GFP and V5

Expression

Mammalian

Promoter

CMV

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMD2.G-VSV-G-mut

Plasmid

#196508

Purpose

VSV-G mutant (K47Q, R354A)

Depositor

Howard Chang

Publication

Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

Insert

VSV-G

Tags

NA

Expression

Mammalian

Mutation

K47Q, R354A

Promoter

CMV

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCRISPRa_all-in-one

Plasmid

#183695

Purpose

Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

Depositor

Edward Avezov

Publication

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Type

Empty backbone

Use

CRISPR

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pRRL.SIN.EF1A.CD19-FMC63.218.CAR-3G

Plasmid

#194458

Purpose

CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.

Depositor

Ana Sofia Coroadinha

Publication

Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

Insert

Anti-CD19 CAR

Use

Lentiviral

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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psPax2-D64V-NC-mNeon

Plasmid

#196509

Purpose

mNeon fluorescent protein fused to nucleocapsid protein in psPAX2

Depositor

Howard Chang

Publication

Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

Insert

mNeon

Tags

mNeon

Expression

Mammalian

Promoter

CMV

Available since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pB025

Plasmid

#183092

Purpose

Expresses FnCas12a in Bacteroides and used for genome editing

Depositor

Lei Dai

Publication

Zheng et al ACS Synth Biol. 2022 Jan 21;11(1):464-47

Insert

FnCas12a, gRNA, Promoter, HAB, TetR,

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

P1TDPGH023

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKS070 - pCAGGS-3XFLAG-(human)CTCF-eGFP

Plasmid

#156448

Purpose

For transient expression of human CTCF tagged with eGFP

Depositor

Elphege Nora

Publication

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

human CTCF (CTCF Human)

Expression

Mammalian

Mutation

none

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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