We narrowed to 6,562 results for: nls
-
Plasmid#57245PurposeLocalization: Membrane Scaffold, Excitation: 400 / 490, Emission: 468 / 511DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPCNA-19 SV40NLS (PCNA Human)
TagsPS-CFP2ExpressionMammalianPromoterCMVAvailable sinceApril 1, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
miRFP703-optoTAT
Plasmid#255924Purposefor use with optoTAT systemDepositorInsertPacI-NheI(kozak)-NLS(c-Myc)X2-miRFP703-LEXY-GS-BamHI-GS-ATAT1(M1-S236)-Stop-NotI
ExpressionMammalianPromoterCMVAvailable sinceAug. 13, 2026AvailabilityAcademic Institutions and Nonprofits only -
lenti-miRFP703-optoTAT
Plasmid#255927Purposefor use with optoTAT systemDepositorInsertPacI-NheI(kozak)-NLS(c-Myc)X2-miRFP703-LEXY-GS-BamHI-GS-ATAT1(M1-S236)-Stop-NotI
UseLentiviralExpressionMammalianPromotermouse CMVAvailable sinceJune 9, 2026AvailabilityAcademic Institutions and Nonprofits only -
mCherry-optoTAT
Plasmid#255923Purposefor use with optoTAT systemDepositorInsertPacI-NheI(kozak)-NLS(c-Myc)X2-mCherry-LEXY-GS-BamHI-GS-ATAT1(M1-S236)-Stop-NotI
ExpressionMammalianPromoterCMVAvailable sinceMay 29, 2026AvailabilityAcademic Institutions and Nonprofits only -
mVenus-optoTAT
Plasmid#255922Purposefor use with optoTAT systemDepositorInsertPacI-NheI(kozak)-NLS(c-Myc)X2-mVenus-LEXY-GS-BamHI-GS-ATAT1(M1-S236)-Stop-NotI
ExpressionMammalianPromoterCMVAvailable sinceMay 18, 2026AvailabilityAcademic Institutions and Nonprofits only -
ARB366
Plasmid#124049PurposeEncodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to KRAB domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vectorDepositorInsertPB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::KRAB-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
ExpressionMammalianAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
ARB367
Plasmid#124050PurposeEncodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to VPR domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vectorDepositorInsertPB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::VPR-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
ExpressionMammalianAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-PEmax
Plasmid#187893PurposeAll-in-one prime editor piggyBac transposon with PEmaxDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpCas9_H840A_KK-MMLV-RT_co-P2A-PAC_dTK
UseCRISPR and Synthetic Biology; Piggybac transposonTagsBPSV40 NLS and c-Myc NLSExpressionMammalianPromoterCAGAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAsCpf1(TYCV)(BB) (pY211)
Plasmid#89352PurposeExpresses humanized AsCpf1 TYCV PAM variant and crRNA guideDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCpf1, RR variant
UseCRISPRTagsNLS and NLS-3xHAExpressionMammalianMutationS542R/K607RPromoterCBhAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-CMV-SV40NLS-OgeuIscBE193A-3XHA
Plasmid#222861PurposeThis plasmid codes for the OgeuIscB nickaseDepositorPublicationInsertOgeuIscB Nickase
UseCRISPRTags3X HA and Nucleoplasmin NLS and ITR2ExpressionMammalianMutationE193A for nickase mutationPromoterCMVAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-alphaCaMKII-dsRed-Express-3XNLS-pA
Plasmid#183802PurposeAAV vector to express nuclear localized dsRed-Express from CaMKIIa promoterDepositorInsertdsRed-Express
UseAAVTagsNLSAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
3xNLS-SGFP2(T65G)-T2A-SGFP2-3xNES
Plasmid#60492PurposeCo-expresses two green fluorescent protein variants, a short fluorescence lifetime GFP variant in the nucleus and a GFP variant with a long lifetime in the cytoplasmDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserts3xnls-SGFP2(T65G)
SGFP2-3xNES
TagsSGFP2 and SGFP2(T65G)ExpressionMammalianPromoterCMVAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SUV[SET]-dCas9
Plasmid#100088PurposeCatalytic domain [SET] of human SUVAR39H1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSUVAR39H1 (SUV39H1 Human)
UseCRISPRTags3XFLag-NLS-SUV[SET]-dCas9-NLSExpressionMammalianMutationdeleted aa 1-76PromoterCMVAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pePEmax-pPuro
Plasmid#215688PurposeCloning vector for Uni-Vector prime editing systemDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPEmax
UseUnspecifiedPromoterEFS promoterAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIIS928
Plasmid#238211PurposeExpresses TaTasR with an N-terminal HA-NLS tag under CMV promoter in human cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTaTasR
TagsHA-NLSExpressionMammalianAvailable sinceMay 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpCas9-VRQR-P2A-mScarlet (MNW004)
Plasmid#174139PurposeCMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarletDepositorInserthuman codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-mScarlet
UseIn vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-mScarletExpressionMammalianMutationVRQR=D1135V/G1218R/R1335Q/T1337RPromoterCMV and T7Available sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCW-Tet-Cas9-BFP-PGK-Blast
Plasmid#196720PurposeTet-inducible expression of Cas9-T2A-TagBFPDepositorInsertCas9-T2A-TagBFP
UseCRISPR and LentiviralTags3xFLAG-SV40NLS and Nucleoplasmin NLSPromoterTRE, PGKAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUS2-SH231
Plasmid#115150PurposeCas9-GFP, dual gRNA expression vector for targeted integration of repair templates into safe harbor 231 locus in chromosome 4DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDual SH231 gRNAs
UseCRISPRTags3xFLAG-SV40NLS, GFP, and Nucleoplasmin NLSExpressionMammalianPromoterU6, CBhAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
His-CL7-2NLS-SpyCas9(dS)-A22p3star
Plasmid#222970PurposeBacterial expression of A22p-fused SpyCas9 constructDepositorInsertHis-CL7-2NLS-SpyCas9(dS)-A22p3
UseCRISPRTagsCL7 and HisExpressionBacterialPromotertacAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-SV40NLSf-mScarletP2A-hLuc
Plasmid#218793PurposeAAV reporter genome plasmidDepositorInsertNLSf-mScarletP2A-hLuc
UseAAVAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only