We narrowed to 8,099 results for: Dos;

1. pCMV-myc-CD4-Sema4D

   Plasmid

   #51603

   Purpose

   expresses Sema4D/CD4 chimera with intracellular Sema4D, extracellular CD4

   Depositor

   Suzanne Paradis

   Article

   Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

   Insert

   CD4-Sema4D
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Sema4D amino acids 1-754 have been replaced with …

   Promoter

   CMV

   Available since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pSMP-MBD1_3

   Plasmid

   #36367

   Depositor

   George Daley

   Article

   Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

   Insert

   MBD1 (MBD1 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pENTR-L3-eSpCas9n(1.1)-2A-EGFP-L2

   Plasmid

   #141052

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   eSpCas9(1.1)-2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-4T-1-p19-T7-GagB500

   Plasmid

   #46314

   Depositor

   Judy Lieberman

   Article

   Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

   Inserts

   p19
   GagB500 DNA-1
   linker
   GagB500 DNA-2
   

   Use

   Pro-sirna

   Tags

   6XHis and GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

5. p5A1-Ti

   Plasmid

   #92018

   Purpose

   Expresses TALE targeted to lac operator with 3 TEV cut sites in its repeat domain, anhydrotetracycline inducible catalytically inactive TEV protease

   Depositor

   Brian Pfleger

   Article

   Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

   Inserts

   TALE targeting lac operator with 3 TEV cleavage sites
   Catalytically Inactive TEV Protease
   

   Use

   TALEN

   Tags

   5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

   Expression

   Mutation

   S219V, C151A

   Promoter

   Available since

   Aug. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK5_tAKARζ

   Plasmid

   #119918

   Purpose

   Mammalian expression of the tAKARζ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

   Depositor

   Haining Zhong

   Article

   Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

   Insert

   tAKARζ
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEM4Z globin NB Probe

   Plasmid

   #120390

   Purpose

   Allows in vitro SP6 Polymerase driven transcription of a beta globin northern blot probe

   Depositor

   Niels Gehring

   Article

   Voigt et al Nat Protoc. 2019 May;14(5):1603-1633. do

   Insert

   beta globin partial 3' UTR
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDH-SIRT6-G60A

   Plasmid

   #102326

   Purpose

   Human SIRT6 G60A for mammalian expression

   Depositor

   Hening Lin

   Article

   Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

   Insert

   SIRT6 (SIRT6 Human)
   

   Use

   Lentiviral

   Tags

   None

   Expression

   Mammalian

   Mutation

   changed glycine 60 to alanine

   Promoter

   CMV

   Available since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOCC194

   Plasmid

   #118882

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal SNAP tag, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3C

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-NotI-ccdB-AscI-TEV-SNAP-3C-HIS6-stop-HindIII cassette
   

   Use

   Tags

   SNAP tag, cleavable with TEV protease, HIS6, clea…

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOCC230

    Plasmid

    #118871

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal mCitrine, cleavable with TEV

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C--NotI-ccdB-AscI-TEV-mCitrine-stop-HindIII cassette
    

    Use

    Tags

    HIS6, cleavable with 3C protease and mCitrine, cl…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pOCC69

    Plasmid

    #118874

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal monomeric GFP, cleavable with TEV

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C--NotI-ccdB-AscI-TEV-mGFP-stop-HindIII cassette
    

    Use

    Tags

    HIS6, cleavable with 3C protease and mGFP, cleava…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pOCC231

    Plasmid

    #118885

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal mCitrine, cleavable with TEV

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-mCitrine-stop-HindIII cassette
    

    Use

    Tags

    HIS6-MBP, cleavable with 3C protease and mCitrine…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSMP-NR2F1_2

    Plasmid

    #36363

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    NR2F1 (NR2F1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pRS426-TUB1-internal6xHis (1-440)

    Plasmid

    #60391

    Purpose

    Expression of yeast alpha tubulin (TUB1) - internal 6xHis without Cterminal tail using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB1 (TUB1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    internal 6xHis (see supplement figure 4), amino a…

    Promoter

    GAL

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pCK025

    Plasmid

    #105415

    Purpose

    Synthetic biology

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Insert

    Hormone-binding domain and regulatory sequences (estrogen receptor)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI/ BpiI restriction sites removed

    Promoter

    Available since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFusX1-31

    Plasmid

    #63249

    Purpose

    Encodes RVD positions 1-3 in TALEN assembly

    Depositor

    Stephen Ekker

    Article

    Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

    Insert

    HD NG NN
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    none

    Promoter

    Available since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pSUPER.retro.puro-shMePCE

    Plasmid

    #113536

    Purpose

    Retroviral vector designed to knock down MePCE.

    Depositor

    Blerta Xhemalce

    Article

    Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

    Insert

    shMePCE (MEPCE Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. Ubiquitin (1-76; L71Y)

    Plasmid

    #110756

    Purpose

    Bacterial expression of human Ubiquitin (1-76; L71Y)

    Depositor

    David Komander

    Article

    Gladkova et al EMBO J. 2017 Dec 15;36(24):3555-3572. do

    Insert

    UBB (UBB Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    L71Y

    Promoter

    T7

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. RCAN1-aa1-150

    Plasmid

    #65412

    Purpose

    RCAN1 aa1-150 expression with CMV promoter. Flag tagged.

    Depositor

    Jeffery Molkentin

    Article

    Liu et al Nat Cell Biol. 2009 Feb;11(2):154-61. do

    Insert

    RCAN1-aa1-150 (Rcan1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    aa1-150 present, deleted 151 to end

    Promoter

    Available since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pSMP-NR2F1_3

    Plasmid

    #36364

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    NR2F1 (NR2F1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only