We narrowed to 13,612 results for: crispr cas9

1. HUHe variant click editor (CE1) - pCMV-T7-GuCV-nCas9-EcKlenow (JO1279)

   Plasmid

   #217788

   Purpose

   Variant CE1 construct with GuCV HUH endonuclease, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   GuCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. HUHe variant click editor (CE1) - pCMV-T7-PCV1-nCas9-EcKlenow (JO1315)

   Plasmid

   #217789

   Purpose

   Variant CE1 construct with PCV1 HUH endonuclease, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV1-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBK2046-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA1)

   Plasmid

   #223166

   Purpose

   2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA1 targeting human/mouse APOE

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   KRAB-MECP2 (MECP2 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 204-310 (MECP2)

   Promoter

   EF1a

   Available Since

   Aug. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV hUbC-Cas9-P2A-Puro_NFATC2-sgRNA

   Plasmid

   #188704

   Purpose

   Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and 4 sgRNAs targeting human NFATC2

   Depositor

   David Tobin

   Article

   Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

   Insert

   NFATC2 sgRNAs
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hUbC

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDY156: SUZ12 px330 sgRNA Cas9 plasmid

   Plasmid

   #170792

   Purpose

   This plasmid encodes Cas9 and a sgRNA that targets the SUZ12 locus; to be used with pDY153

   Depositor

   Thomas Cech

   Article

   Youmans et al Mol Cell. 2021 Feb 4;81(3):488-501.e9. d

   Insert

   Cas9
   

   Expression

   Mammalian

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCB-CRISPRi

   Plasmid

   #221136

   Purpose

   Coxiella burnetii CRISPRi plasmid without sgRNA construct. Expresses 3xF-dCas9.

   Depositor

   Craig Roy

   Article

   Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

   Insert

   3xF-dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Bacterial

   Promoter

   cbu1169 promoter

   Available Since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. px335-sgRNA-EF(Cas9n)-Dnmt3b-R

   Plasmid

   #122333

   Purpose

   Expresses sgRNA targeting mouse Dnmt3b and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Dnmt3b
   

   Expression

   Mammalian

   Available Since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. px335-sgRNA-EF(Cas9n)-Dnmt3b-L

   Plasmid

   #122334

   Purpose

   Expresses sgRNA targeting mouse Dnmt3b and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Dnmt3b
   

   Expression

   Mammalian

   Available Since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. px335-sgRNA-EF(Cas9n)-Eif4a1-R

   Plasmid

   #122345

   Purpose

   Expresses sgRNA targeting mouse Eif4a1 and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Eif4a1
   

   Expression

   Mammalian

   Available Since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. px335-sgRNA-EF(Cas9n)-Eif4a1-L

    Plasmid

    #122346

    Purpose

    Expresses sgRNA targeting mouse Eif4a1 and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Eif4a1
    

    Expression

    Mammalian

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. px335-sgRNA-EF(Cas9n)-Ddx42-L

    Plasmid

    #122343

    Purpose

    Expresses sgRNA targeting mouse Ddx42 and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Ddx42
    

    Expression

    Mammalian

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. px335-sgRNA-EF(Cas9n)-Ddx42-R

    Plasmid

    #122344

    Purpose

    Expresses sgRNA targeting mouse Ddx42 and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Ddx42
    

    Expression

    Mammalian

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. px335-sgRNA-EF(Cas9n)-Ncapd3-R

    Plasmid

    #122330

    Purpose

    Expresses sgRNA targeting mouse Ncapd3 and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Ncapd3
    

    Expression

    Mammalian

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. px335-sgRNA-EF(Cas9n)-Npm1-R

    Plasmid

    #122331

    Purpose

    Expresses sgRNA targeting mouse Npm1 and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Npm1
    

    Expression

    Mammalian

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. px335-sgRNA-EF(Cas9n)-Npm1-L

    Plasmid

    #122332

    Purpose

    Expresses sgRNA targeting mouse Npm1 and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Npm1
    

    Expression

    Mammalian

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. px335-sgRNA-EF(Cas9n)-Dhx15-R

    Plasmid

    #122335

    Purpose

    Expresses sgRNA targeting mouse Dhx15 and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Dhx15
    

    Expression

    Mammalian

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. px335-sgRNA-EF(Cas9n)-Dhx15-L

    Plasmid

    #122336

    Purpose

    Expresses sgRNA targeting mouse Dhx15 and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Ddx15
    

    Expression

    Mammalian

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. px335-sgRNA-EF(Cas9n)-Birc6-L

    Plasmid

    #122337

    Purpose

    Expresses sgRNA targeting mouse Birc6 and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Birc6
    

    Expression

    Mammalian

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. px335-sgRNA-EF(Cas9n)-Birc6-R

    Plasmid

    #122338

    Purpose

    Expresses sgRNA targeting mouse Birc6 and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Birc6
    

    Expression

    Mammalian

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. px335-sgRNA-EF(Cas9n)-Trim28-R

    Plasmid

    #122339

    Purpose

    Expresses sgRNA targeting mouse Trim28 and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Trim28
    

    Expression

    Mammalian

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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