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pGEX6P1-EGFP-anti-rabbit-IgG(Fc)-nanobody

Plasmid

#138133

Purpose

Expresses GST-tagged EGFP-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

EGFP-anti-rabbit-IgG(Fc)-nanobody

Tags

GST

Expression

Bacterial

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPspCas13b-EGFP-NLS-3xFlag

Plasmid

#132397

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1058A

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-Rubicon

Plasmid

#221659

Purpose

Rubicon tagged N-terminally with EGFP; clone a gift from the Hurley Lab at UC Berkeley

Depositor

Erika Holzbaur

Article

Basak et al Nat Commun. 2025 Aug 9;16(1):7367. doi:

Insert

RUBCN (RUBCN Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-Rubicon

Plasmid

#221657

Purpose

Rubicon tagged N-terminally with mCherry

Depositor

Erika Holzbaur

Article

Basak et al Nat Commun. 2025 Aug 9;16(1):7367. doi:

Insert

RUBCN (RUBCN Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-mCherry-anti-rabbit-IgG(Fc)-nanobody

Plasmid

#138131

Purpose

Expresses GST-tagged mCherry-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

mCherry-anti-rabbit-IgG(Fc)-nanobody

Tags

GST

Expression

Bacterial

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry_P2AT2A_GFP-nb-KS-FtoA

Plasmid

#238234

Purpose

For overexpression of mCherry_P2AT2A_GFP-nb-KS-FtoA

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry_P2AT2A_GFP-nb-KS-FtoA

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-6015nes

Plasmid

#108657

Purpose

A S6 Kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

Depositor

Michiyuki Matsuda

Article

Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

Insert

hyBRET-S6K-EV

Tags

XFP

Expression

Mammalian

Promoter

pCAGGS

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry-RPL18

Plasmid

#238253

Purpose

For overexpression of mCherry-RPL18

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry-RPL18

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-axon-GCaMP6f

Plasmid

#112000

Purpose

For enriched expression of GCaMP6f in axons

Depositor

Lin Tian

Article

Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

Has Service

AAV1

Insert

axon-GCaMP6f

Use

AAV

Tags

GAP43 palmitoylation domain

Expression

Mammalian

Promoter

hSynapsin1

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-B-GECO1

Plasmid

#32448

Purpose

Mammalian expression of blue fluorescent genetically encoded Ca2+ indicator for optical imaging

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

B-GECO1.0

Expression

Mammalian

Mutation

GCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D30…

Promoter

CMV

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only

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CMV-G-GECO1.1

Plasmid

#32445

Purpose

Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.1)

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

G-GECO1.1

Expression

Mammalian

Mutation

GCaMP3 K69E/N77Y/D86G/K119I/L173Q/K380N/S404G/E43…

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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PB_TetON_mCherry_P2AT2A_GFP-nb-KS-FtoA

Plasmid

#238241

Purpose

For integration of mCherry_P2AT2A_GFP-nb-KS-FtoA

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry_P2AT2A_GFP-nb-KS-FtoA

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pFast-hHus1

Plasmid

#222469

Purpose

Expresses hHus1 in insect cells

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al Proc Natl Acad Sci U S A. 2001 Sep 25;98

Insert

hHus1 (HUS1 Human)

Expression

Insect

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.tgoGAP1

Plasmid

#78737

Purpose

Expression of Ca2+ sensor GAP1 in the trans Golgi apparatus

Depositor

Teresa Alonso

Article

Rodriguez-Garcia et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

Insert

tgoGAP1

Tags

N terminal 81 amino acids of human galactosyltran…

Expression

Mammalian

Mutation

D117A/D119A/D163A in aequorin moiety

Promoter

CMV

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
pRSET-WHaloCaMP1a

Plasmid

#205304

Purpose

Expression of WHaloCaMP1a in E. coli

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP1a

Expression

Bacterial

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pSW002-Pc-DsRed-Express2

Plasmid

#111251

Purpose

Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with DsRed-Express2

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

DsRed-Express2

Expression

Bacterial

Promoter

Pc

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-pAceR

Plasmid

#195521

Purpose

Red fluorescent, positive response-polarity voltage indicator under the control of synapsin promoter

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

pAceR

Use

AAV

Expression

Mammalian

Mutation

VARNAM 78E, 81D, 92N; SI linker

Promoter

Synapsin

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS_cAMPFIRE-M

Plasmid

#182284

Purpose

Mammalian expression of the cAMP sensor cAMPFIRE-M under a CAG promotor w/ WPRE translation enhancer.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-M (cAMP sensor)

Expression

Mammalian

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-N-TEV-HAT

Plasmid

#202598

Purpose

Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Type

Empty backbone

Tags

TEV cleavable site (ENLYFQG) and poly-histidine a…

Expression

Mammalian

Promoter

CAG

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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PCDNA3-FlipGFP(Caspase-1 cleavage seq) T2A mCherry

Plasmid

#124494

Purpose

Expresses FlipGFP (caspase-1 cleavage sequence) and T2A mCherry

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

FlipGFP(Caspase-1 cleavage seq) T2A mCherry

Use

Synthetic Biology

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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