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Degron-KI p53 donor vector

Plasmid

#65619

Purpose

Donor vector to generate Degron-KI DD-p53

Depositor

Frank Stegmeier

Article

Zhou et al Cancer Res. 2015 Mar 18. pii: canres.293

Insert

p53 Degron-KI left and right HR arms

Available Since

July 13, 2015

Availability

Academic Institutions and Nonprofits only
pENTR-Rabin8

Plasmid

#24910

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

Rabin8 (RAB3IP Human)

Use

Gateway entry vector

Available Since

July 7, 2010

Availability

Academic Institutions and Nonprofits only

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p416GPD-PYK1

Plasmid

#34604

Depositor

Markus Ralser

Article

Gruning et al Cell Metab. 2011 Sep 7;14(3):415-27.

Insert

PYK1

Expression

Yeast

Promoter

GPD

Available Since

Jan. 11, 2012

Availability

Academic Institutions and Nonprofits only

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Degron-KI ezh2 donor vector

Plasmid

#65620

Purpose

Donor vector to generate Degron-KI DD-EZH2

Depositor

Frank Stegmeier

Article

Zhou et al Cancer Res. 2015 Mar 18. pii: canres.293

Insert

EZH2 left and right HR arms

Available Since

July 24, 2015

Availability

Academic Institutions and Nonprofits only
P413TEF-PYK1

Plasmid

#34605

Depositor

Markus Ralser

Article

Gruning et al Cell Metab. 2011 Sep 7;14(3):415-27.

Insert

PYK1

Expression

Yeast

Promoter

TEF

Available Since

Jan. 11, 2012

Availability

Academic Institutions and Nonprofits only

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DPY-pNCS

Plasmid

#17365

Depositor

Stephen Goff

Article

Yuan et al J Virol. 2000 Aug . 74(16):7250-60.

Insert

MMLV proviral DNA

Mutation

deletion that removes 5 amino acids (DPPPY) from …

Available Since

March 1, 2010

Availability

Academic Institutions and Nonprofits only
Degron-KI PIK3CA donor vector

Plasmid

#65621

Purpose

Donor vector to generate Degron-KI DD-PIK3CA

Depositor

Frank Stegmeier

Article

Zhou et al Cancer Res. 2015 Mar 18. pii: canres.293

Insert

PIK3CA Degron-KI left and right HR arms

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
pENTR-fishRab8A[T22N]

Plasmid

#24906

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

fishRab8A

Use

Gateway entry vector

Mutation

Changed Threonine 22 to Asparagine

Available Since

July 7, 2010

Availability

Academic Institutions and Nonprofits only
p413TEF-PYK2

Plasmid

#34606

Depositor

Markus Ralser

Article

Gruning et al Cell Metab. 2011 Sep 7;14(3):415-27.

Insert

PYK2 (PYK2 Budding Yeast)

Expression

Yeast

Promoter

TEF

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pENTR-fishRab8A[Q67L]

Plasmid

#24905

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

fishRab8A

Use

Gateway entry vector

Mutation

Changed Glutamine 67 to Leucine

Available Since

June 28, 2010

Availability

Academic Institutions and Nonprofits only
pENTR-fishRab8B[T22N]

Plasmid

#24909

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

fishRab8B

Use

Gateway entry vector

Mutation

Changed Threonine 22 to Asparagine

Available Since

June 28, 2010

Availability

Academic Institutions and Nonprofits only
pC1-HyPerRed-mito

Plasmid

#60247

Purpose

Mitochondria-targeted genetically encoded hydrogen peroxide indicator with red fluorescence.

Depositor

Vsevolod Belousov

Article

Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

Insert

HyPerRed-mito

Tags

Mitochondrial targeting signal

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pETM6-G6-mCherry

Plasmid

#66533

Purpose

Expresses mCherry under the control of Mutant T7 Promoter G6

Depositor

Mattheos Koffas

Article

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Insert

mCherry

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon Optimized for E. coli

Promoter

Mutant T7 Promoter - G6

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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pLV hU6-gRNA(anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFP

Plasmid

#66707

Purpose

expresses a single gRNA and VP64-dCas9-VP64

Depositor

Charles Gersbach

Inserts

hU6-gRNA expression cassette
S.Pyogenes VP64-dCas9-VP64

Use

Lentiviral and Synthetic Biology

Tags

VP64

Mutation

D10A and H840A

Promoter

hU6 and hUbC

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pC1-HyPer-Red-C199S

Plasmid

#48252

Purpose

Hydrogen peroxide insensitive version of HyPerRed to be used as a pH control in HyPer experiments.

Depositor

Vsevolod Belousov

Article

Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

Insert

HYPER-RED-C199S

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-3HA-His3MX6

Plasmid

#41600

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

His3

Use

Yeast genomic targeting

Tags

3xHA, A. gossypii translation elongation factor 1…

Available Since

May 15, 2013

Availability

Academic Institutions and Nonprofits only

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SB52

Plasmid

#21487

Depositor

Shoumo Bhattacharya

Article

Bhattacharya et al Genes Dev. 1999 Jan 1. 13(1):64-75.

Insert

Cited2 (CITED2 Human)

Expression

Mammalian

Available Since

July 23, 2009

Availability

Academic Institutions and Nonprofits only
pFA6a-13Myc-His3MX6

Plasmid

#41602

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

His3

Use

Yeast genomic targeting

Tags

13Myc, A. gossypii translation elongation factor …

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pETM6-H10-mCherry

Plasmid

#66531

Purpose

Expresses mCherry under the control of Mutant T7 Promoter H10

Depositor

Mattheos Koffas

Article

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Insert

mCherry

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon Optimized for E. coli

Promoter

Mutant T7 Promoter - H10

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-GST-His3MX6

Plasmid

#41604

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

His3

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge