We narrowed to 4,668 results for: pcr

1. pCR4-*mRFP

   Plasmid

   #47118

   Purpose

   Can be used to make an RNA probe for whole-mount in situ hybridization, for in-frame fusion proteins or as a subcloning step for mRFP. Note that the coding sequence for mRFP lacks the start codon.

   Depositor

   Stephen Ekker

   Article

   Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub

   Insert

   *mRFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   No start codon

   Promoter

   Lac

   Available since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCR2.1-SNORD116-3

   Plasmid

   #45891

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Clin Med Insights Case Rep. 2013 May 5;6

   Insert

   SNORD115 with 80nt from intron sequence but without flanking exons (SNORD116-3 Human)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pCR2.1-rap55

   Plasmid

   #26154

   Depositor

   Phillip Newmark

   Article

   Wang et al Genes Dev. 2010 Sep 15. 24(18):2081-92.

   Insert

   Smed-rap55
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pCR2.1-eIF3c

   Plasmid

   #26153

   Depositor

   Phillip Newmark

   Article

   Wang et al Genes Dev. 2010 Sep 15. 24(18):2081-92.

   Insert

   Smed-eIF3c
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 16, 2010

   Availability

   Academic Institutions and Nonprofits only

5. PCRII-zSynA

   Plasmid

   #20121

   Depositor

   Aaron Gitler

   Insert

   zSynA (sncgb Zebrafish)
   

   Use

   In-situ

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 20, 2009

   Availability

   Academic Institutions and Nonprofits only

6. PCRII-zSynB

   Plasmid

   #20122

   Depositor

   Aaron Gitler

   Insert

   zSynB (sncga Zebrafish)
   

   Use

   In-situ

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pBS598 EF1alpha-EGFPcre

   Plasmid

   #11923

   Purpose

   Expresses EGFP-Cre fusion under the control of the EF1alpha promoter; EGFP is human codon-optimized.

   Depositor

   Brian Sauer

   Article

   Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

   Insert

   EGFP-cre (cre bacteriophage P1)
   

   Use

   Cre/Lox

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   March 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCR8/GW/TOPO-TBX20a

   Plasmid

   #98620

   Purpose

   Gateway entry TOPO TA cloning vector for TBX20a

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   Tbx20a (Tbx20 Mouse)
   

   Use

   Topo ta cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCR4-24XMS2SL-stable

   Plasmid

   #31865

   Depositor

   Robert Singer

   Article

   Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

   Insert

   24xMS2 Stem Loops - Stable
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 31, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRIS-PITChv2-FBL

    Plasmid

    #63672

    Purpose

    PITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locus

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

    Insert

    EGFP-2A-PuroR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Promoterless

    Available since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPEY-GAL-Nat

    Plasmid

    #232097

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with NatMX selection

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCrysβ:ECFP-LexOP:mCherry-KrasG12V

    Plasmid

    #89888

    Purpose

    Human KRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

    Insert

    mCherry-KRasG12V
    

    Use

    Unspecified

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-TadA8e-PcrA

    Plasmid

    #221228

    Purpose

    Express HACE Editor as a fusion of TadA-8e adenosine deaminase and PcrA helicase.

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    TadA8e-PcrA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPaint-mNeon-PuroR

    Plasmid

    #174090

    Purpose

    CRISPaint gene tagging

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    mNeonGreen
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRISPR-PsppA-mCherry

    Plasmid

    #225428

    Purpose

    Plasmid encodes SgRNA, SpCas9 and homologous arms for homologous recombination of mCherry

    Depositor

    Geir Mathiesen

    Article

    Wiull et al Microbiol Spectr. 2025 Feb 4;13(2):e0202

    Insert

    mCherry, Cas9, SgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PsppA upstream of mCherry and Cas9, P3 upstream o…

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCry2PHR-mCh-LRP6c

    Plasmid

    #42960

    Purpose

    Expresses optogenetically controlled LRP6 (C-terminal domain)

    Depositor

    David Schaffer

    Article

    Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

    Insert

    Cry2PHR-mCherry-LRP6c
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    LPR6 cytoplasmic domain only

    Promoter

    CMV

    Available since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCR3.1-mGM-CSF

    Plasmid

    #74465

    Purpose

    expression of mGM-CSF in mammalian cells

    Depositor

    Loree Heller

    Article

    Heller et al Melanoma Res. 2000 Dec;10(6):577-83.

    Insert

    granulocyte-monocyte colony-stimulating factor (Csf2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCR3-FLAG-Gephyrin P1

    Plasmid

    #68816

    Purpose

    Flag tagged Geph mammalian expression

    Depositor

    Shiva Tyagarajan

    Article

    Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

    Insert

    Gephyrin (Gphn Rat)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    silent mutations on internal EcoRI site. Gephyrin…

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pCRISPEY-GAL-Kan

    Plasmid

    #232099

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with KanMX selection

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCRISPEY-GAL-Hyg

    Plasmid

    #232098

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with HygMX selection

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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