We narrowed to 1,096 results for: pcs2

1. pCS2+ H2B-mEmerald

   Plasmid

   #99269

   Purpose

   Contains a fusion of human histone H2B to fluorescent protein mEmerald in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

   Depositor

   Philipp Keller

   Insert

   mEmerald
   

   Use

   Tags

   H2B

   Expression

   Mammalian

   Mutation

   Promoter

   SP6

   Available Since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2+ MBIC5-mCer3

   Plasmid

   #74116

   Purpose

   For generating mRNA for zebrafish injection of cytoplasmic fluorogen-activating protein (FAP)

   Depositor

   Michael Tsang

   Article

   He et al Nat Methods. 2016 Mar;13(3):263-268. doi

   Insert

   MBIC5-mCer3
   

   Use

   Transgenic construct

   Tags

   mCerulean 3

   Expression

   Mutation

   Promoter

   Myl7 (Cmlc2)

   Available Since

   May 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2-mCherry-MKLP1

   Plasmid

   #140562

   Purpose

   Expresses mCherry tagged MKLP1

   Depositor

   Heidi Hehnly

   Article

   Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

   Insert

   MKLP1 (KIF23 Human)
   

   Use

   Tags

   2xmCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available Since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2-Opto-Alk8

   Plasmid

   #207615

   Purpose

   Zebrafish Alk8 BMP receptor kinase domain fused to VfLOV

   Depositor

   Patrick Müller

   Article

   Rogers et al Elife. 2020 Nov 11;9:e58641. doi: 10.755

   Insert

   Alk8 (acvr1l) kinase domain + VfLOV domain (acvr1l Vaucheria frigida, Zebrafish)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2-USP44-HIS

   Plasmid

   #43894

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

   Insert

   USP44 (USP44 Human)
   

   Use

   Tags

   HIS

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 12, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pCS2+ cMyc Dam_f_GFPoNLS

   Plasmid

   #85820

   Purpose

   iDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

   Depositor

   Joachim Wittbrodt

   Article

   Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

   Insert

   cMyc Dam_f_GFPoNLS
   

   Use

   Tags

   cMyc and oNLS (optimized nuclear localization sig…

   Expression

   Bacterial, Insect, Mammalia…

   Mutation

   L122A.

   Promoter

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2-H2B-mCherry

   Plasmid

   #135604

   Purpose

   Expression of H2B-mCherry mRNA for microinjection to visualize Histone 2B protein dynamics in S. purpuratus

   Depositor

   Mamiko Yajima

   Article

   Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

   Insert

   H2B-mCherry
   

   Use

   In vitro transcription (mrna synthesis)

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Unknown

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS2 xRor2-Flag

   Plasmid

   #67164

   Purpose

   mammalian expression / in vitro transcription

   Depositor

   Alexandra Schambony

   Article

   Feike et al Biochem Biophys Res Commun. 2010 Oct 1;4

   Insert

   Tyrosine-protein kinase transmembrane receptor ROR2 (ror2 Frog)
   

   Use

   In vitro transcription

   Tags

   FLag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 16, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pCS2+8NCerulean-ABCB1a

   Plasmid

   #34944

   Depositor

   Amro Hamdoun

   Article

   Gokirmak et al J Biol Chem. 2012 Nov 2.

   Insert

   ABCB1a
   

   Use

   Sea urchin, zebrafish, xenopus

   Tags

   Cerulean

   Expression

   Mammalian

   Mutation

   Promoter

   SP6

   Available Since

   Jan. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pCS2-SP-FKBP-mCherry-TM-V5

    Plasmid

    #186226

    Purpose

    mRNA synthesis of rapalog-induced cell adhesion molecule

    Depositor

    Yoshihiro Morishita

    Article

    Usami et al Genes Cells. 2022 Jun;27(6):436-450. doi

    Insert

    SP-FKBP-mCherry-TM-V5
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2+methylated-reporter

    Plasmid

    #164491

    Purpose

    Reporter to test methylated mRNA stability, for mRNA injection

    Depositor

    Antonio Giraldez

    Article

    Kontur et al Cell Rep. 2020 Dec 29;33(13):108598. doi

    Insert

    A-less CDS, HA tag, 12x m6A motif 3'UTR
    

    Use

    Production of mrna for zebrafish injection.

    Tags

    HA tag

    Expression

    Mutation

    Promoter

    SP6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS2+-dCas9-olEzh2

    Plasmid

    #180216

    Purpose

    Ezh2 fused to dCas9 for H3K27me3 epigenome editing

    Depositor

    Hiroyuki Takeda

    Article

    Fukushima et al Epigenetics Chromatin. 2019 Mar 13;12(1)

    Insert

    enhancer of zeste 2 polycomb repressive complex 2 subunit
    

    Use

    Tags

    FLAG and dCas9

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2 xenopus Insulin 2

    Plasmid

    #25786

    Depositor

    Edward De Robertis

    Article

    Pera et al Mech Dev. 2002 Aug . 116(1-2):169-72.

    Insert

    Insulin 2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pCS2-hFAM 3B-His

    Plasmid

    #172192

    Purpose

    Coding sequence of human FAM3B pCS2 C terminal 6xHis tag used for protein purification from conditioned medium

    Depositor

    Edward De Robertis

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2021 May 18;11

    Insert

    hFAM 3B
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCS2+ cMyc-oDam_f_GFPoNLS

    Plasmid

    #85818

    Purpose

    iDamID plasmid. To express transiently the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

    Depositor

    Joachim Wittbrodt

    Article

    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

    Insert

    cMyc oDam_f_GFPoNLS
    

    Use

    Tags

    cMyc and oNLS (optimized nuclear localization sig…

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    L122A. Codon optimization compatible to work in M…

    Promoter

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. ZE48 Dvl3 pCS2P+

    Plasmid

    #17089

    Depositor

    Randall Moon

    Article

    Waxman et al Development. 2004 Dec. 131(23):5909-21.

    Insert

    Dvl-3 (dvl3a Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pCS2+ Dsh (Xenopus)

    Plasmid

    #13438

    Depositor

    Marc Kirschner

    Article

    Lee et al J Cell Biol. 2001 Sep 3. 154(5):983-93.

    Insert

    Dsh (dvl2.L Frog)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

18. V50 pCS2+mRFP-N3

    Plasmid

    #17145

    Depositor

    Randall Moon

    Type

    Empty backbone

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

19. ZE41 Dapper-1 pCS2P+

    Plasmid

    #17082

    Depositor

    Randall Moon

    Article

    Waxman et al Development. 2004 Dec. 131(23):5909-21.

    Insert

    Dapper-1 (dact1 Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

20. ZE43 Dapper-2 pCS2P+

    Plasmid

    #17084

    Depositor

    Randall Moon

    Article

    Waxman et al Development. 2004 Dec. 131(23):5909-21.

    Insert

    Dapper-2 (dact2 Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only