We narrowed to 22,217 results for: tes

1. p3E-csChBP

   Plasmid

   #108893

   Purpose

   Multisite gateway vector for 3' tagging with a conditionally stable nanobody against mCherry

   Depositor

   Rob Parton

   Article

   Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

   Insert

   dChBP
   

   Use

   Multisite gateway

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOZ-N-FH RAP80

   Plasmid

   #27501

   Depositor

   Roger Greenberg

   Article

   Patterson-Fortin et al J Biol Chem. 2010 Oct 1. 285(40):30971-8

   Insert

   RAP80 (UIMC1 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 1, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSB1C3-Lux

   Plasmid

   #109383

   Purpose

   Arabinose inducible expression of the Vibrio Fischery Lux operon. Used to test burden response in E. coli

   Depositor

   Tom Ellis

   Article

   Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

   Insert

   pARABAD-LUX operon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTalpha1-iCre

   Plasmid

   #133924

   Purpose

   Neuron-specific expression of iCre

   Depositor

   Frank Bradke, Sebastián Dupraz

   Article

   Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

   Insert

   iCre
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Codon optimized

   Promoter

   neuron-specific tubulin-alpha-1 promoter

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. H2B-mCherry

   Plasmid

   #108880

   Purpose

   Expression of human histone2B fused to mCherry (nuclear marker)

   Depositor

   Rob Parton

   Article

   Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

   Insert

   H2B-mCherry
   

   Use

   Can be used for mammalian expression or in vitro …

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFP-P2A-APEX2-ChBP

   Plasmid

   #108879

   Purpose

   Expression of APEX2 fused to a nanobody against mCherry (includes green cytoplasmic reporter)

   Depositor

   Rob Parton

   Article

   Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

   Insert

   EGFP-P2A-APEX2-ChBP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28b-pchG WT

   Plasmid

   #87107

   Purpose

   expresses PchG WT, the thiazolinyl imine reductase from Pseudomonas aeruginosa

   Depositor

   Audrey Lamb

   Article

   Meneely et al Biochemistry. 2016 Sep 27;55(38):5423-33

   Insert

   pchG WT
   

   Use

   Tags

   his-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. mKate2-P2A-APEX2-csGBP

   Plasmid

   #108875

   Purpose

   Expression of APEX2 fused to a conditionally stable nanobody against GFP (includes red cytoplasmic reporter)

   Depositor

   Rob Parton

   Article

   Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

   Insert

   mKate2-P2A-APEX2-csGBP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. p3E-APEX2-P2A-EGFP

   Plasmid

   #108896

   Purpose

   Multisite gateway vector for 3' tagging with APEX2 (includes cytoplasmic GFP reporter)

   Depositor

   Rob Parton

   Article

   Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

   Insert

   APEX2-P2A-EGFP
   

   Use

   Multisite gateway

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCSN075

    Plasmid

    #166732

    Purpose

    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS and Mxi, codon optimized for Saccharomyces cerevisiae

    Depositor

    Rene Verwaal

    Article

    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

    Insert

    dCas12a-NLS-Mxi1
    

    Use

    CRISPR

    Tags

    Mxi1 and SV40 NLS

    Expression

    Yeast

    Mutation

    E925A

    Promoter

    S. cerevisiae TEF1

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. 114_UBI_iCre_crystGFP

    Plasmid

    #171797

    Purpose

    Tol2-plasmid ubiquitously expressing iCre (ubiquitin promoter) along with eGFP selection marker (Crystallin promoter -Lens/Eyes-)

    Depositor

    Jean Giacomotto

    Article

    Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

    Insert

    optimised iCre under the control of the Ubiquitin promoter
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Ubiquitin

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pME-secAnxaV-NS

    Plasmid

    #67718

    Purpose

    Gateway entry clone containing secreted human AnxaV with no stop codon

    Depositor

    Rob Parton

    Article

    Morsch et al Front Cell Neurosci. 2015 Aug 31;9:321.

    Insert

    secAnxaV-NS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLys-M1

    Plasmid

    #109382

    Purpose

    Arabinose inducible VioB-mCherry expression (stronger RBS). Used to test burden response in E. coli

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    pARABAD-VioB-mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-CMV-tagRFP-Mad2

    Plasmid

    #114021

    Purpose

    Lentiviral delivery of tagRFP-MAD2 expressed from the CMV promoter

    Depositor

    Geert Kops

    Article

    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

    Insert

    tagRFP-Mad2 (MAD2L1) (MAD2L1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 1457_pDEST_miniTol2_R4-R2_Cryst_mCherry

    Plasmid

    #171796

    Purpose

    pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a mCherry selection marker (Crystallin promoter Lens/Eyes)

    Depositor

    Jean Giacomotto

    Article

    Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. zbFGF

    Plasmid

    #12309

    Depositor

    James Thomson

    Article

    Ludwig et al Nat Methods. 2006 Aug . 3(8):637-646.

    Insert

    Fgf2 (fgf2 Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Ala 11 mutated to Pro, Ser 55 mutated to Asn

    Promoter

    Available since

    Sept. 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBAD-jRCaMP1a

    Plasmid

    #113936

    Purpose

    Bacterial expression of red fluorescent Ca2+ sensor jRCaMP1a

    Depositor

    Robert Campbell, Thomas Hughes

    Article

    Molina et al Biophys J. 2019 May 21;116(10):1873-1886

    Insert

    jRCaMP1a
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EGFP-P2A-APEX2-csChBP

    Plasmid

    #108877

    Purpose

    Expression of APEX2 fused to a conditionally stable nanobody against mCherry (includes green cytoplasmic reporter)

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    EGFP-P2A-APEX2-csChBP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pME-mKate2-NS

    Plasmid

    #109729

    Purpose

    Multisite gateway vector for 5' tagging with mKate2

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    mKate2
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBAD-R-GECO1.2

    Plasmid

    #113937

    Purpose

    Bacterial expression of red fluorescent Ca2+ sensor R-GECO1.2

    Depositor

    Robert Campbell, Thomas Hughes

    Article

    Molina et al Biophys J. 2019 May 21;116(10):1873-1886

    Insert

    R-GECO1.2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge