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pHR-EF1a-Archon1-KGC-EGFP-ER2

Plasmid

#108425

Purpose

Lentivirus production plasmid encoding for Archon1 fluorescent voltage reporter

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

Archon1-KGC-EGFP-ER2

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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5xUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

Plasmid

#158651

Purpose

making transgenics expressing a UAS driven YFP and NTR 2.0

Depositor

Jeff Mumm

Article

Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

Insert

5xUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y;he:BFP

Use

Tol2-based transgenic expression in zebrafish/oth…

Mutation

F70A/F108Y

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-Archon1-KGC-EGFP

Plasmid

#108418

Purpose

AAV production plasmid encoding for Archon1 fluorescent voltage reporter

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

Archon1-KGC-EGFP

Use

AAV

Expression

Mammalian

Promoter

CaMKII

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pTol2-UAS-zmiRFP

Plasmid

#108415

Purpose

Codon-optimized monomeric near-infrared fluorescent protein miRFP in zebrafish expression plasmid

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

zmiRFP

Use

Zebrafish

Promoter

UAS

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pGFP-mHP1alpha

Plasmid

#181900

Purpose

Fluorescently tagged HP1alpha

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

mHP1alpha (Cbx5 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-F-Med26-NTD R61A K62A

Plasmid

#49256

Purpose

Mammalian expression of flag-tagged human Med26-NTD R61A K62A

Depositor

Joan Conaway, Ronald Conaway

Article

Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

Insert

Med 26 (MED26 Human)

Tags

Flag

Expression

Mammalian

Mutation

NTD R61A K62A

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-Syn-miRFP

Plasmid

#108416

Purpose

Monomeric near-infrared fluorescent protein miRFP in mammalian-expressing AAV production plasmid

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

miRFP

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pmiRFP-N1

Plasmid

#108409

Purpose

Monomeric near-infrared fluorescent protein miRFP in mammalian expression plasmid

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

miRFP

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pGFP-mHP1gamma

Plasmid

#181902

Purpose

Fluorescently tagged HP1gamma

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

mHP1gamma (Cbx3 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-EF1a-Archon2-KGC-EGFP-ER2

Plasmid

#108426

Purpose

Lentivirus production plasmid encoding for Archon2 fluorescent voltage reporter

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

Archon2-KGC-EGFP-ER2

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-miRFP

Plasmid

#108410

Purpose

Monomeric near-infrared fluorescent protein miRFP in bacterial expression plasmid

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

miRFP

Expression

Bacterial

Promoter

araB

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pET28-GFP-mHP1alpha

Plasmid

#181912

Purpose

Bacterial protein expression vector for GFP-tagged HP1alpha

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

mHP1alpha (Cbx5 Mouse)

Tags

GFP

Expression

Bacterial

Promoter

T7

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pmiRFP-beta-Actin-C-19

Plasmid

#108412

Purpose

Beta-actin fused to monomeric near-infrared fluorescent protein miRFP

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

beta-Actin

Tags

miRFP

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pSF143-wArchon2-KGC-EGFP-ER2

Plasmid

#108430

Purpose

Codon-optimized Archon2 fluorescent voltage reporter in C.elegans expression plasmid

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

wArchon2-KGC-EGFP-ER2

Expression

Worm

Promoter

flp-18

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delWGR-L713F

Plasmid

#173941

Purpose

Bacterial expression of PARP1 lacking WGR domain but containing L713F gain-of-function mutation

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delWGR-L713F (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 521-660; L713F, V762A

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFPc1 PTEN C124S

Plasmid

#237340

Purpose

transient overexpression in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nature. 2017 Jun 22;546(7659):554-558. d

Insert

PTEN (PTEN Human)

Expression

Mammalian

Mutation

C124S

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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ERAP1-rs27895(T)

Plasmid

#206143

Purpose

Expresses the rs27895-T allele of ERAP1, myc-tagged.

Depositor

Dennis Ko

Article

Schott et al Cell Genom. 2022 Nov 9;2(11):100207. doi

Insert

ERAP1 (ERAP1 Human)

Tags

myc

Mutation

G346 changed to D346

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pMECP2-RFP

Plasmid

#181905

Purpose

Fluorescently tagged MECP2, RFP

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

MECP2 (Mecp2 Mouse)

Tags

RFP

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pTRE3G-ZSGreen1-mHP1alpha

Plasmid

#181913

Purpose

Inducible mammalian expression vector for ZsGreen1-tagged HP1alpha

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

mHP1alpha (Cbx5 Mouse)

Tags

ZSGreen1

Expression

Mammalian

Promoter

TRE3G

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delZn3-L713F

Plasmid

#173934

Purpose

Bacterial expression of PARP1 lacking Zn3 domain but containing L713F gain-of-function mutation

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delZn3-L713F (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 214-373; L713F, V762A

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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