We narrowed to 11,600 results for: 112

1. pRK5-myc-Miro1 T18N

   Plasmid

   #47890

   Purpose

   Expresses myc tagged Miro1 T18N dominant negative mutant

   Depositor

   Pontus Aspenström

   Article

   Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502

   Insert

   Miro1 T18N (RHOT1 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   T18N, dominant negative mutant

   Promoter

   CMV

   Available since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pMXs-IP spGFP-ERGIC53

   Plasmid

   #38270

   Depositor

   Noboru Mizushima

   Article

   Itakura et al Autophagy. 2010 Aug;6(6):764-76.

   Insert

   ERGIC-53 (LMAN1 Human)
   

   Use

   Retroviral

   Tags

   EGFP with Calreticulin signal peptide at N-termin…

   Expression

   Mammalian

   Mutation

   deleted signal peptide (1-30 amino acids)

   Promoter

   Available since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCI-Neo-PTPRT (WT)

   Plasmid

   #16630

   Depositor

   Bert Vogelstein

   Article

   Wang et al Science. 2004 May 21. 304(5674):1164-6.

   Insert

   PTPRT (PTPRT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

4. delCMV-mCherry-2x-Rhotekin-GBD

   Plasmid

   #214142

   Purpose

   Rho activity sensor

   Depositor

   Leif Dehmelt

   Article

   Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi:

   Insert

   3xRhotekin-GBD (Rtkn Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Only the GTPase binding domain (GBD) is included

   Promoter

   delCMV

   Available since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1+DPP6

   Plasmid

   #117272

   Purpose

   Encodes human DPP6 variant 1

   Depositor

   Mark Cannell

   Article

   Lainez et al Biochem Pharmacol. 2018 Apr;150:120-130.

   Insert

   DPP6 (DPP6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. GPR44-Tango

   Plasmid

   #66359

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   GPR44 (PTGDR2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pGEX-6P-1-hs-ALPS 1-ALPS2-EGFP

   Plasmid

   #187113

   Purpose

   Protein Expression

   Depositor

   Philipp Niethammer

   Article

   Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

   Insert

   ENSG00000101199 (ARFGAP1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRK5-myc-Miro1 P13V

   Plasmid

   #47889

   Purpose

   Expresses myc tagged Miro1 P13V constitutively active mutant

   Depositor

   Pontus Aspenström

   Article

   Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502

   Insert

   Miro1 P13V (RHOT1 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   P13V, constitutively active mutant

   Promoter

   CMV

   Available since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pEF-GalNAc-T2-msGFP2

   Plasmid

   #140164

   Purpose

   Expresses the Golgi marker GalnacT2-msGFP2

   Depositor

   Benjamin Glick

   Article

   Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

   Insert

   GalNAc-T2-msGFP2 (GALNT2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHY002_Strep_Avi_hsDCAF15

    Plasmid

    #174479

    Purpose

    Expresses DCAF15 in insect cells

    Depositor

    Eric Fischer

    Article

    Faust et al Nat Chem Biol. 2020 Jan;16(1):7-14. doi:

    Insert

    DCAF 15 (DCAF15 Human)
    

    Use

    Tags

    StrepII-Avi-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Flag p21 T145A

    Plasmid

    #16241

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2001 Mar . 3(3):245-52.

    Insert

    p21 (CDKN1A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T145A

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

12. Flag p21 T145D

    Plasmid

    #16242

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2001 Mar . 3(3):245-52.

    Insert

    p21 (CDKN1A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T145D

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

13. ankG-mCherry

    Plasmid

    #42566

    Depositor

    Benedicte Dargent

    Article

    Leterrier et al Proc Natl Acad Sci U S A. 2011 May 24;10

    Insert

    ankyrin G (Ank3 Rat)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-myc3-bTrCP

    Plasmid

    #20718

    Depositor

    Yue Xiong

    Article

    Ohta et al Cancer Res. 2001 Feb 15. 61(4):1347-53.

    Insert

    beta-transducin repeat containing (BTRC) (Btrc Mouse)
    

    Use

    Tags

    Myc3

    Expression

    Mammalian

    Mutation

    *

    Promoter

    Available since

    May 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSL3927 (pDonor(plasmid)_Pse)

    Plasmid

    #200905

    Purpose

    Encodes a mini-transposon derived from Pseudoalteromonas Tn7016,with a CmrR cargo, as well as a primer binding site for deep sequencing purposes at pTarget. Total transposon size = 1,087 bp.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    Mini Tn7016 (pTarget NGS)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223 LC3A WT

    Plasmid

    #123069

    Purpose

    Gateway entry clone encoding human MAP1LC3A wild-type

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
    

    Use

    Gateway entry vector / entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. 1255_pAAV-U6-SA-BbsI-MluI-gRNA-CB-SACas9-HA-OLLAS-spA

    Plasmid

    #109320

    Purpose

    Plasmid for AAV SaCas9 Mammalian Expression with a BbsI cloning site for a gRNA

    Depositor

    William Lagor

    Article

    Jarrett et al Arterioscler Thromb Vasc Biol. 2018 Jul

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLentiCRISPRv1 sgTollip

    Plasmid

    #196546

    Purpose

    Lentiviral CRISPR-Cas9 plasmid containing gRNA targeting exon 1 of human Tollip. Used for generation of Tollip protein knockouts in human cell lines.

    Depositor

    David Tumbarello

    Article

    Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

    Insert

    Toll-Interacting Protein (TOLLIP Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-p58

    Plasmid

    #166942

    Purpose

    mammalian expression of p58 (ie ERGIC-53; LMAN1) tagged with GFP

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Ward et al J Cell Biol. 2001 Nov 12;155(4):557-70.

    Insert

    p58 (Lman1 Rat)
    

    Use

    Tags

    GFP and chicken lysozyme signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET19-MTdTwt

    Plasmid

    #207460

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    MTdTwt (Dntt Mouse)
    

    Use

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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