We narrowed to 3,684 results for: Gla

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BCD2_BC

Plasmid

#66024

Purpose

MoClo Basic Part: RBS - BiCistronic Design, high strength (RBS part type, actually contains a small transcriptional unit and second RBS) [B:BCD2:C]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

RBS

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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BCD8_BC

Plasmid

#66025

Purpose

MoClo Basic Part: RBS - BiCistronic Design, low strength (RBS part type, actually contains a small transcriptional unit and second RBS) [B:BCD8:C]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

RBS

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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C0040 (tetR)_CD

Plasmid

#66027

Purpose

MoClo Basic Part: CDS - Controller protein, tetR repressor (represses pTet, C0040. can be inhibited by tetracyclin or aTc) [C:C0040:D]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Transcription factor

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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C0080 (araC)_CD

Plasmid

#66029

Purpose

MoClo Basic Part: CDS - Controller protein, araC repressor/activator (activates pBAD, I13453) [C:C0080:D]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Transcription factor

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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R0010 (pLacI)_EB

Plasmid

#66005

Purpose

MoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [E:R0010:B]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Controllable promoter

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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R0010 (pLacI)_FB

Plasmid

#66006

Purpose

MoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [F:R0010:B]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Controllable promoter

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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R0010 (pLacI)_GB

Plasmid

#66007

Purpose

MoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [G:R0010:B]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Controllable promoter

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pSC-hXRCC1-K176R-IntE2

Plasmid

#52277

Purpose

Bacterial expression of human XRCC1-K176R C-terminally fused to GyrA intein, SUMO-E2 and GST

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

XRCC1 (XRCC1 Human)

Tags

GyrA-intein-Ubc9-GST

Expression

Bacterial

Mutation

K176R, SUMO acceptor site mutant

Promoter

T7

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pSC-hXRCC1-K176R-PreE2

Plasmid

#52279

Purpose

Bacterial expression of human XRCC1-K176R C-terminally fused to SUMO-E2 and GST

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

XRCC1 (XRCC1 Human)

Tags

Ubc9-GST

Expression

Bacterial

Mutation

K176R, SUMO acceptor site mutant

Promoter

T7

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pTYB-pLAC-F112S

Plasmid

#48727

Purpose

Bacterial expression of Lacritin Protein with Mutation at protein 112 from Phenylalanine to Serine

Depositor

Gordon Laurie

Article

Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

Insert

Lacritin (LACRT Human)

Tags

Intein

Expression

Bacterial

Mutation

Phenylalanine 12 to Serine

Promoter

T7

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only
pTYB1-pLAC-I98S

Plasmid

#48723

Purpose

Bacterial expression of Lacritin Protein with Mutation at protein 98 from Isoleucine to Serine

Depositor

Gordon Laurie

Article

Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

Insert

Lacritin (LACRT Human)

Tags

Intein

Expression

Bacterial

Mutation

Isoleucine 98 to Serine

Promoter

T7

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only
pTYB1-pLAC-I73S

Plasmid

#48722

Purpose

Bacterial expression of Lacritin Protein with Mutation at protein 73 from Isoleucine to Serine

Depositor

Gordon Laurie

Article

Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

Insert

Lacritin (LACRT Human)

Tags

Intein

Expression

Bacterial

Mutation

Isoleucine 73 to Serine

Promoter

T7

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only
pTYB1-pLAC-V69S

Plasmid

#48721

Purpose

Bacterial expression of Lacritin Protein with Mutation at protein 69 from Valine to Serine

Depositor

Gordon Laurie

Article

Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

Insert

Lacritin (LACRT Human)

Tags

Intein

Expression

Bacterial

Mutation

Valine 69 to Serine

Promoter

T7

Available Since

Oct. 18, 2013

Availability

Academic Institutions and Nonprofits only
KHBD00045

Plasmid

#34207

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG12952 (sage Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
hMLKL NB(1-182)-2xFv-Venus

Plasmid

#106077

Purpose

expression in mammalian cells

Depositor

Douglas Green

Article

Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

Insert

human MLKL NB(1-182)-2xFv-Venus (MLKL Human)

Use

Retroviral; Tet-on retrovirus

Tags

Venus

Expression

Mammalian

Mutation

amino acids 1-182

Promoter

Dox-inducible

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-NES-jRGECO1a

Plasmid

#61563

Purpose

Red fluorescent calcium sensor protein

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Insert

NES-jRGECO1a

Tags

nuclear export signal

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-GCaMP6s

Plasmid

#40753

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Insert

GCaMP6s

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-NES-jRCaMP1a

Plasmid

#61562

Purpose

Red fluorescent calcium sensor protein

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Insert

NES-jRCaMP1a

Tags

nuclear export signal

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.NES-jRGECO1a.WPRE.SV40

Plasmid

#100854

Purpose

AAV expression of jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, and AAV9

Insert

jRGECO1a

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-GCaMP6f

Plasmid

#40755

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Insert

GCaMP6f

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

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