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pAS_4xEcoLeuT(CUA)_EF1_sfGFP150TAG

Plasmid

#174892

Purpose

amber suppression reporter sfGFP150TAG with EcoLeuT(CUA) amber suppressor tRNA cassette

Depositor

Simon Elsaesser

Publication

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG in sfGFP

Promoter

EF1

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pNCST-AvicFP2

Plasmid

#129505

Purpose

Expresses AvicFP2 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Publication

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AvicFP2

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pNCST-AvicFP4

Plasmid

#129507

Purpose

Expresses AvicFP4 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Publication

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AvicFP4

Expression

Bacterial

Mutation

One of two variants of AvicFP4 tested with essent…

Promoter

synthetic constitutive (stationary phase) promote…

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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LifeAct-mAvicFP1

Plasmid

#129512

Purpose

Expresses LifeAct-mAvicFP1 in mammalian cells

Depositor

Nathan Shaner

Publication

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LifeAct-mAvicFP1

Expression

Mammalian

Mutation

mAvicFP1 is AvicFP1 F64L + A206K

Promoter

CMV

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pNCST-AausFP3

Plasmid

#129501

Purpose

Expresses AausFP3 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Publication

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AausFP3

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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MTK1_023

Plasmid

#123685

Purpose

Encodes ConL1 and human B1 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L1- B1 insulator

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_024

Plasmid

#123686

Purpose

Encodes ConL2 and human C3 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L2-C3 insulator

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK2_001

Plasmid

#123697

Purpose

Encodes pTRE as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pTRE

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK2_006

Plasmid

#123701

Purpose

Encodes pSV40 as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pSV40

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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p3E-TAPP1-PH

Plasmid

#67675

Purpose

Multisite gateway entry clone for adding C-terminal fusions of TAPP1-PH

Depositor

Rob Parton

Publication

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

TAPP1-PH

Use

Gateway multisite 3' entry clone

Available since

June 14, 2017

Availability

Academic Institutions and Nonprofits only

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p3E-ML1N*2(7Q)

Plasmid

#67679

Purpose

Multisite gateway entry clone for adding C-terminal fusions of p3E-ML1N*2(7Q)

Depositor

Rob Parton

Publication

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

ML1N*2(7Q)

Use

Gateway multisite 3' entry clone

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pBbS5a-OptoCre-REDMAP

Plasmid

#228465

Purpose

Light-inducible Cre recombinase split with REDMAP photoreceptors for bacterial expression

Depositor

Mary Dunlop

Publication

Jafarbeglou et al ACS Synth Biol. 2024 Nov 19. doi: 10.102

Insert

OptoCre-REDMAP

Use

Cre/Lox

Expression

Bacterial

Promoter

pLacUV5

Available since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBbAk-w4-loxP-TT-loxP-mCherry-PCB

Plasmid

#228469

Purpose

Fluorescent reporter for Cre recombinase activity and the PCB production gene. Cre removes a transcription terminator, leading to mCherry production

Depositor

Mary Dunlop

Publication

Jafarbeglou et al ACS Synth Biol. 2024 Nov 19. doi: 10.102

Inserts

w4-loxP-TT-loxP-mCherry
Ho1
PcyA

Expression

Bacterial

Promoter

J23106, J23108, and T7A1

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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iMbody_pSANG10-3F

Plasmid

#184496

Purpose

Expresses iMbody-6xHis-3xFLAG in BL21(DE3) bacteria

Depositor

Marcel Dinger

Publication

Zeraati et al STAR Protoc. 2023 Nov 21;4(4):102729. do

Insert

iMbody

Tags

3xFLAG, 6xHis, and pelB leader

Expression

Bacterial

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-PD-1 G224A-mGFP

Plasmid

#180819

Purpose

Expression of human PD-1 G224A fused to mEGFP

Depositor

Enfu Hui

Publication

Xu et al Elife. 2021 Nov 4;10. pii: 74276. doi: 1

Insert

PD-1 (PDCD1 Human)

Use

Lentiviral

Tags

mEGFP

Mutation

G224A

Promoter

SFFV

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pmCherry-p23

Plasmid

#108224

Purpose

Mammalian expression vector for mCherry fusion to human p23

Depositor

Didier Picard

Publication

Picard et al Exp Cell Res. 2006 Nov 15. 312(19):3949-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCherry-p23 (PTGES3 Human)

Expression

Mammalian

Promoter

CMV

Available since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pRN3P-HA-Suv4-20h2

Plasmid

#86691

Purpose

For transcription of of Suv4-20h2 mRNA preceded by an HA-tag in 5'

Depositor

Maria-Elena Torres-Padilla

Publication

Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

Insert

histone-lysine N-methyltransferase KMT5C (Kmt5c Mouse)

Tags

HA

Promoter

T3

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV-Myc-XPLN

Plasmid

#73365

Purpose

Expresses myc tagged XPLN in mammalian cells

Depositor

Jie Chen, Krister Wennerberg

Publication

Arthur et al J Biol Chem. 2002 Nov 8;277(45):42964-72

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

arhgef3 (ARHGEF3 Human)

Tags

Myc (QKLISEEDL)

Expression

Mammalian

Promoter

CMV

Available since

April 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
tdEos-Talin-18

Plasmid

#57672

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Publication

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Talin

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article
U6>Control(F+E)

Plasmid

#60006

Purpose

U6 promoter driving control (F+E) sgRNA

Depositor

Lionel Christiaen

Publication

Stolfi et al Development. 2014 Nov;141(21):4115-20. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Control (F+E) sgRNA

Use

CRISPR

Promoter

Ciinte.U6

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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