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pFUW-SNAP25B-V48F

Plasmid

#248130

Purpose

Mammalian expression lentiviral vector encoding the Synaptosomal-Associated Protein (SNAP-25) with V48F mutation

Depositor

Ege Kavalali

Article

Alten et al Neuron. 2021 Jan 6;109(1):59-72.e5. doi:

Insert

SNAP25B-V48F (SNAP25 Human)

Expression

Mammalian

Mutation

V48F

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-SNAP25B-I67N

Plasmid

#248125

Purpose

Mammalian expression lentiviral vector encoding the Synaptosomal-Associated Protein (SNAP-25) with I67N mutation

Depositor

Ege Kavalali

Article

Alten et al Neuron. 2021 Jan 6;109(1):59-72.e5. doi:

Insert

SNAP25B-I67N (SNAP25 Human)

Expression

Mammalian

Mutation

I67N

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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p-att-ef1a-MS2-RecT-dCas9-BSD

Plasmid

#226108

Purpose

Using ef-1a promoter, expresses dCas9 and RecT protein that intended to be tethered via MS2 gRNA hairpin to dCas9, and Blasticidin selection

Depositor

George Church

Article

Oliynyk et al Commun Biol 8 (2025). https://doi.org/10

Insert

BSD

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pEF1-FRT-HaloTag-TAF1A

Plasmid

#247347

Purpose

Expresses HaloTag-TAF1A

Depositor

Kazuhiro Maeshima

Article

Ide et al Sci Adv. 2020 Oct 14;6(42). pii: 6/42/ea

Insert

TAF1A (TAF1A) (TAF1A Human)

Tags

HaloTag

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pEM2_Euk_2NLS-RVQ (LbCas12a)-2NLS

Plasmid

#244837

Purpose

Mammalian expression of LbCas12a-RVQ with 2xSV40 NLS at N-terminus and 2xSV40 at C-terminus

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

LbCas12a-RVQ

Use

CRISPR

Tags

2xSV40 NLS

Expression

Mammalian

Mutation

G146R/R182V/E795Q

Promoter

T7

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pHS641_Flex-Cas12a-ABE

Plasmid

#244846

Purpose

Mammalian expression of Flex-Cas12a adenine base editor

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

Flex-dCas12a-ABE

Use

CRISPR

Tags

SV40 NLS-ABE8e

Expression

Mammalian

Mutation

G146R/R182V/D535G/S551F/D665N/E795Q/D832A

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SpRY-IP

Plasmid

#235994

Purpose

Expresses Cas9-SpRY in mammalian cells

Depositor

Tobias Cantz

Article

Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

SpRY

Use

CRISPR

Expression

Mammalian

Promoter

EF-1a

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pACUH 5-HT2BR-7xGFP11-HA

Plasmid

#221840

Purpose

Plasmid for generating 10xUAS-5-HT2BR-7xGFP11-HA transgenic flies with attP/attB integration. It carries the attB sequence.

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

5-HT2BR (CG42796 Fly)

Tags

7xGFP11-HA

Expression

Insect

Promoter

hsp70

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn:FLEX-Soma-jGCaMP8m

Plasmid

#239670

Purpose

Cre-dependent expression of Soma-jGCaMP8m under hSyn promoter.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

Soma-jGCaMP8m

Use

AAV

Tags

6xHis and EE-RR

Expression

Mammalian

Promoter

hSyn

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn:FLEX-Soma-jGCaMP8s

Plasmid

#239669

Purpose

Cre-dependent expression of Soma-jGCaMP8s under hSyn promoter.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

Soma-jGCaMP8s

Use

AAV

Tags

6xHis and EE-RR

Expression

Mammalian

Promoter

hSyn

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn:FLEX-Soma-jGCaMP8f

Plasmid

#239671

Purpose

Cre-dependent expression of Soma-jGCaMP8f under hSyn promoter.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

Soma-jGCaMP8f

Use

AAV

Tags

6xHis and EE-RR

Expression

Mammalian

Promoter

hSyn

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_V5-nb-KS

Plasmid

#238266

Purpose

For overexpression of V5-nb-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

V5-nb-KS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1_-DIO-Varnam1-WPRE

Plasmid

#240054

Purpose

Cre dependent Red fluorescent voltage indicator for mammalian expression

Depositor

Mark Schnitzer

Article

Haziza et al Cell, 188, 1-23, August 7, 2025. doi: 10

Insert

VARNAM1

Use

AAV

Promoter

Ef1a

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-Varnam2-WPRE

Plasmid

#240055

Purpose

Cre dependent Red fluorescent, negative response-polarity voltage indicator for mammalian expression

Depositor

Mark Schnitzer

Article

Haziza et al Cell, 188, 1-23, August 7, 2025. doi: 10

Insert

VARNAM2

Use

AAV

Promoter

Ef1a

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pPSmF

Plasmid

#226912

Purpose

Minimal plasmid for Plant STARR-seq with enhancer candidates in forward orientation

Depositor

Stan Fields

Article

Jores et al Plant Cell. 2025 Jun 4;37(6):koaf084. do

Type

Empty backbone

Expression

Plant

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pPSmR

Plasmid

#226913

Purpose

Minimal plasmid for Plant STARR-seq with enhancer candidates in reverse orientation

Depositor

Stan Fields

Article

Jores et al Plant Cell. 2025 Jun 4;37(6):koaf084. do

Type

Empty backbone

Expression

Plant

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_V5-nb-noKS

Plasmid

#238265

Purpose

For overexpression of V5-nb-noKS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

V5-nb-noKS

Expression

Mammalian

Available Since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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PB_TetON_mCherry-P2A

Plasmid

#238238

Purpose

For integration of mCherry control

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry

Expression

Mammalian

Available Since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry-P2A

Plasmid

#238231

Purpose

For overexpression of mCherry control

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_V5-EGFP-NPM1

Plasmid

#238264

Purpose

For overexpression of V5-EGFP-NPM1

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

V5-EGFP-NPM1

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry-SURF6

Plasmid

#238252

Purpose

For overexpression of mCherry-SURF6

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry-SURF6

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_V5-nb-KS-FtoG

Plasmid

#238267

Purpose

For overexpression of V5-nb-KS-FtoG

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

V5-nb-KS-FtoG

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_TagBFP-P2A

Plasmid

#238272

Purpose

For overexpression of TagBFP-control

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

TagBFP

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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CT362

Plasmid

#233679

Purpose

Red light-inducible Cre-C; Cre-230C-PhyA

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

Cre-230C-PhyA

Expression

Mammalian

Mutation

None

Promoter

CAG

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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CT611

Plasmid

#233669

Purpose

Red light-inducible Cre-C; Cre-230C-FHY1

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

Cre-230C-FHY1

Use

Cre/Lox

Expression

Mammalian

Mutation

None

Promoter

CAGG

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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CT367

Plasmid

#233673

Purpose

Red light-inducible Cre-N; Cre-N229-FHY1

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

Cre-N229-FHY1

Expression

Mammalian

Mutation

None

Promoter

CAG

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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CT336

Plasmid

#233662

Purpose

AND Gate Reporter to identify Cre and Flp recombination; FRT-STOP-FRT-loxP-STOP-loxP-GFP

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

FRT-STOP-FRT-loxP-STOP-loxP-GFP

Expression

Mammalian

Mutation

None

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p52K-KS-FtoA

Plasmid

#237689

Purpose

For overexpression of 52K-KS-FtoA

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

52K-KS-FtoA

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p52K-linkerKS-FtoA

Plasmid

#237693

Purpose

For overexpression of 52K-linkerKS-FtoA

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

52K-linkerKS-FtoA

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p52K-GFP-KS-FtoA

Plasmid

#237691

Purpose

For overexpression of 52K-GFP-KS-FtoA

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

52K-GFP-KS-FtoA

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p52K-GFP-linker-KS

Plasmid

#237686

Purpose

For overexpression of 52K-GFP-linker-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

52K-GFP-linker-KS

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p52K-linkerKS

Plasmid

#237692

Purpose

For overexpression of 52K-linkerKS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

52K-linkerKS

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EW378 (ZNF35/ZNF250tarv3-6bp spacers)x4-H2B-GFP

Plasmid

#236134

Purpose

Fuorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with four ZNF35/ZNF250 binding sites upstream

Depositor

Xiaojing Gao

Article

Wolfsberg et al bioRxiv 2025.02.24.639918

Insert

H2B (H2BC11 Human)

Tags

EGFP

Expression

Mammalian

Promoter

E1b minimal promoter with four ZNF35/ZNF250 bindi…

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EW408 (ZNF35tar-compact)x8-H2B-GFP

Plasmid

#236129

Purpose

Fuorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with eight ZNF35 binding sites upstream

Depositor

Xiaojing Gao

Article

Wolfsberg et al bioRxiv 2025.02.24.639918

Insert

H2B (H2BC11 Human)

Tags

EGFP

Expression

Mammalian

Promoter

E1b minimal promoter with eight ZNF35 binding sit…

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EW411 (UTRN-43-5bp spacer-UTRN-14)-H2B-GFP

Plasmid

#236148

Purpose

Fluorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with the UTRN promoter region encompassing 14 to 67 bp upstream of its transcriptional start site

Depositor

Xiaojing Gao

Article

Wolfsberg et al bioRxiv 2025.02.24.639918

Insert

H2B (H2BC11 Human)

Tags

EGFP

Expression

Mammalian

Promoter

E1b minimal promoter with the UTRN promoter region

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EW407 (ZNF35/ZNF35-6bp spacers)x4-H2B-GFP

Plasmid

#236137

Purpose

Fuorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with four ZNF35/ZNF250 binding sites upstream

Depositor

Xiaojing Gao

Article

Wolfsberg et al bioRxiv 2025.02.24.639918

Insert

H2B (H2BC11 Human)

Tags

EGFP

Expression

Mammalian

Promoter

E1b minimal promoter with four ZNF35/ZNF250 bindi…

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
SCN1Aprom(1-500)-H2B-GFP

Plasmid

#236162

Purpose

Fluorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with the SCN1a promoter region encompassing 500 bp upstream of its transcriptional start site

Depositor

Xiaojing Gao

Article

Wolfsberg et al bioRxiv 2025.02.24.639918

Insert

H2B (H2BC11 Human)

Tags

EGFP

Expression

Mammalian

Promoter

E1b minimal promoter with the SCN1a promoter regi…

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
SCN1Aprom(335-406)-H2B-GFP

Plasmid

#236151

Purpose

Fluorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with the SCN1a promoter region encompassing 335 to 406 bp upstream of its transcriptional start site

Depositor

Xiaojing Gao

Article

Wolfsberg et al bioRxiv 2025.02.24.639918

Insert

H2B (H2BC11 Human)

Tags

EGFP

Expression

Mammalian

Promoter

E1b minimal promoter with the SCN1a promoter regi…

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EW387 (ZNF35/IKZF1tar-6bp spacers)x4-H2B-GFP

Plasmid

#236138

Purpose

Fuorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with four ZNF35/ZNFIKZF1 binding sites upstream

Depositor

Xiaojing Gao

Article

Wolfsberg et al bioRxiv 2025.02.24.639918

Insert

H2B (H2BC11 Human)

Tags

EGFP

Expression

Mammalian

Promoter

E1b minimal promoter with four ZNF35/ZNFIKZF1 bin…

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-TetO-AncHolozoan Sox

Plasmid

#231528

Purpose

Expresses reconstructed Ancestral Holozoan Sox in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Ancestral Holozoan Sox HMG flanked with N- and C-termini of mSox2

Use

Lentiviral

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-TetO-AncMetazoan Sox

Plasmid

#231527

Purpose

Expresses reconstructed Ancestral Metazoan Sox in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Ancestral Metazoan Sox HMG flanked with N- and C-termini of mSox2

Use

Lentiviral

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-TetO-AncSoxCEF

Plasmid

#231526

Purpose

Expresses reconstructed Ancestral SoxCEF in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Ancestral SoxCEF HMG flanked with N- and C-termini of mSox2

Use

Lentiviral

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-TetO-AncSoxB

Plasmid

#231524

Purpose

Expresses reconstructed Ancestral SoxB in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Ancestral SoxB HMG flanked with N- and C-termini of mSox2

Use

Lentiviral

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-TetO-Salhel-I-Sox2HMG

Plasmid

#231533

Purpose

Expresses chimeric Sox2 HMG in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

mSox2 HMG flanked with N- and C-termini of Salhel-I

Use

Lentiviral

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-TetO-Salhel-Sox-I

Plasmid

#231515

Purpose

Expresses Salpingoeca helianthica full-length Sox-I in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Sox-I

Use

Lentiviral

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-TetO-Myflu-POU

Plasmid

#231523

Purpose

Expresses Mylnosiga fluctuans full-length POU in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

POU

Use

Lentiviral

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-TetO-AncSoxBCEF

Plasmid

#231525

Purpose

Expresses reconstructed Ancestral SoxBCEF in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Ancestral SoxBCEF HMG flanked with N- and C-termini of mSox2

Use

Lentiviral

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-TetO-Chim-hCic

Plasmid

#231529

Purpose

Expresses chimeric human Cic in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Human Cic HMG flanked with N- and C-termini of mSox2

Use

Lentiviral

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-TetO-Sox2-HMG

Plasmid

#231532

Purpose

Expresses mouse Sox2 HMG in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

mSox2 HMG

Use

Lentiviral

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-TetO-Salhel-II-Sox2HMG

Plasmid

#231534

Purpose

Expresses chimeric Sox2 HMG in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

mSox2 HMG flanked with N- and C-termini of Salhel-II

Use

Lentiviral

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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