We narrowed to 4,523 results for: ARA-2

1. pASPIre4

   Plasmid

   #196656

   Purpose

   Derivative of pASPIre3. Contains SpeI restriction site within the CDS of bxb1 to enable diversification of the 5’-UTR and codons 2-16.

   Depositor

   Markus Jeschek

   Article

   Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237

   Insert

   Bxb1-sfGFP fusion controlled by rhamnose promoter; attB/attP-flanked discriminator; exchangable 5'-UTR and CDS (codons 1-16)
   

   Expression

   Bacterial

   Mutation

   SpeI site in Bxb1 CDS

   Promoter

   rhamnose-inducible promoter

   Available Since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. SNAP-Kindlin

   Plasmid

   #186136

   Purpose

   For expression of recombinant Snap-labeled kindlin in bacteria

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   FERMT2 (FERMT2 Human)
   

   Tags

   His6-sumo

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHBS1506 [IBB-GFP-mCherry3E]-[BFP-TDP43 FYW-L]

   Plasmid

   #133330

   Purpose

   To test the effect of sequence on TDP43 splicing activity

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Expression

   Mammalian

   Mutation

   All FYW to L in CTD of full-length TDP43

   Available Since

   Nov. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHBS1397 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 W385G]

   Plasmid

   #118812

   Purpose

   To test the effect of sequence on TDP43 splicing activity

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Expression

   Mammalian

   Mutation

   W385G in CTD of full-length TDP43

   Available Since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHBS1400 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G295F]

   Plasmid

   #118814

   Purpose

   To test the effect of sequence on TDP43 splicing activity

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Expression

   Mammalian

   Mutation

   G295F in CTD of full-length TDP43

   Available Since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHBS1393 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 deltaRRM]

   Plasmid

   #118805

   Purpose

   To test the effect of sequence on TDP43 splicing activity

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Expression

   Mammalian

   Mutation

   Deletion of RRM in full-length TDP43

   Available Since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHBS1394 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 deltaCTD]

   Plasmid

   #118806

   Purpose

   To test the effect of sequence on TDP43 splicing activity

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Expression

   Mammalian

   Mutation

   Deletion of CTD in full-length TDP43

   Available Since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHBS1385 TDP43 RRM-GFP VLIM-S

   Plasmid

   #118792

   Purpose

   To test the effect of sequence on TDP43 RRM-GFP droplet properties

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Expression

   Mammalian

   Mutation

   All VLIM to S in CTD of TDP43 RRM-GFP reporter

   Available Since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHBS1384 TDP43 RRM-GFP FYW-L

   Plasmid

   #118793

   Purpose

   To test the effect of sequence on TDP43 RRM-GFP droplet properties

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Expression

   Mammalian

   Mutation

   All FYW to L in CTD of TDP43 RRM-GFP reporter

   Available Since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHBS1292 TDP43 RRM-GFP G368W+W385G

    Plasmid

    #107838

    Purpose

    To test the effect of sequence on TDP43 droplet properties

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    G368W and W385G mutations in LLPS reporter

    Available Since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHBS1039 TDP43 RRM-GFP 2xΦ

    Plasmid

    #107806

    Purpose

    To test the effect of sequence on TDP43 droplet properties

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    All hydrophobics combined to two clusters in LLPS…

    Available Since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHBS1038 TDP43 RRM-GFP 4xΦ

    Plasmid

    #107805

    Purpose

    To test the effect of sequence on TDP43 droplet properties

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    All hydrophobics combined to four clusters in LLP…

    Available Since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHBS1037 TDP43 RRM-GFP 6xΦ

    Plasmid

    #107804

    Purpose

    To test the effect of sequence on TDP43 droplet properties

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    All hydrophobics combined to six clusters in LLPS…

    Available Since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVITRO1-Trastuzumab-IgG2/κ

    Plasmid

    #61884

    Purpose

    Expresses HER2/neu receptor specific humanized IgG2/κ antibody isotype (Trastuzumab-IgG2/κ)

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    HER2/neu receptor specific humanized Gamma 2 heavy chain expression cassette
    HER2/neu receptor specific humanized kappa light chain expression cassette
    

    Expression

    Mammalian

    Promoter

    mEF1 Prom and rEF1 Prom

    Available Since

    March 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVITRO1-Trastuzumab-IgA2/κ

    Plasmid

    #61889

    Purpose

    Expresses HER2/neu receptor specific humanized IgA2/κ antibody isotype (Trastuzumab-IgA2/κ)

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    HER2/neu receptor specific humanized Alpha 2 heavy chain expression cassette
    HER2/neu receptor specific humanized kappa light chain expression cassette
    

    Expression

    Mammalian

    Promoter

    mEF1 Prom and rEF1 Prom

    Available Since

    March 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHub10-His-YFP-PIP2;1

    Plasmid

    #99386

    Purpose

    overexpression and isolation of recombinant At-PIP2;1 protein

    Depositor

    Ning Li

    Article

    Qing et al Mol Plant. 2016 Jan 4;9(1):158-74. doi:

    Insert

    PIP2;1 (PIP2A Mustard Weed)
    

    Tags

    His and YFP

    Expression

    Plant

    Promoter

    2xCaMV35S

    Available Since

    Aug. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTER GAMMA-TUBULIN shRNA Human

    Plasmid

    #87955

    Purpose

    reduced the expression of gamma-tubulin in human cells

    Depositor

    Maria Alvarado-Kristensson, Ana Carrera

    Article

    Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

    Insert

    sh gamma-tubulin (TUBG1 Human)
    

    Tags

    No-tag

    Expression

    Mammalian

    Mutation

    The protein is a sh-gamma-tubulin resistant gene.…

    Available Since

    Oct. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CRY2-TSS-guide3

    Plasmid

    #125430

    Purpose

    CRISPR-mediated repression of CRY2. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    hPGK and U6

    Available Since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide1

    Plasmid

    #118162

    Purpose

    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA1 against the EGFP CDS

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    hPGK and U6

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR TCF7L2 TSS-guide1

    Plasmid

    #118169

    Purpose

    CRISPR-mediated repression of TCF7L2. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    hPGK and U6

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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