We narrowed to 11,259 results for: HAL;

1. pSAVED-CHAT_PCaspase-N-term

   Plasmid

   #214044

   Purpose

   Bacterial expression plasmid for SAVED-CHAT and PCaspase N-terminal fragment (aa 1-153) from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT-PCaspase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   aa 1-153

   Promoter

   lacUV5 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSAVED-CHAT_PCaspase-C-term

   Plasmid

   #214045

   Purpose

   Bacterial expression plasmid for SAVED-CHAT and PCaspase C-terminal fragment (aa 154-666) from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT-PCaspase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   aa 154-666

   Promoter

   lacUV5 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSAVED-CHAT_PCaspase_PCi

   Plasmid

   #214049

   Purpose

   Bacterial expression plasmid for SAVED-CHAT, PCaspase, and PCi from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT-Pcaspase-PCi
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   lacUV5 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJS-BCD-Strep-PC-σ

   Plasmid

   #214037

   Purpose

   Bacterial expression plasmid for strep-tagged PC-σ from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   PC-σ
   

   Use

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. 1BR9-AtWUSSTOP-Δα3

   Plasmid

   #202056

   Purpose

   E. coli expression vector for N-ter GFP11 tagged Arabidopsis thaliana WUSCHEL with the third helix in the homeodomain deleted.

   Depositor

   Markita Landry

   Article

   Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

   Insert

   AtWUS E. Coli Codon Optimized, third helix of homeodomain deleted (WUS Mustard Weed)
   

   Use

   Tags

   6xHis and GFP11

   Expression

   Bacterial

   Mutation

   third helix of homeodomain deleted (AA82-102)

   Promoter

   T7 Promoter

   Available since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. fru-T2A-QF2 HDR plasmid

   Plasmid

   #141099

   Purpose

   Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti fru gene

   Depositor

   Leslie Vosshall

   Article

   Basrur et al Elife. 2020 Dec 7;9. pii: 63982. doi: 10

   Insert

   fru-left-HDR-arm; T2A-QF2-hsp70; 3xP3-dsRed-SV40; fru-right-HDR-arm
   

   Use

   Donor template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDONR207-MUM2sp-Citrine-ADPG2

   Plasmid

   #170725

   Purpose

   Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the Citrine and the coding sequence of the HG degrading enzyme ADPG2 (At2g41850.1)

   Depositor

   George Haughn

   Article

   McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 629

   Insert

   ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2 (PGAZAT Mustard Weed)
   

   Use

   Gateway donor vector / entry clone

   Tags

   Citrine tag (714bp)

   Expression

   Mutation

   Promoter

   Available since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDONR207-MUM2sp-ADPG2

   Plasmid

   #170723

   Purpose

   Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the HG degrading enzyme ADPG2 (At2g41850.1)

   Depositor

   George Haughn

   Article

   McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 629

   Insert

   ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2 (PGAZAT Mustard Weed)
   

   Use

   Gateway donor vector / entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 11, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pX022

   Plasmid

   #167148

   Purpose

   MoClo-compatible Level 0-CDS2 promoterless vector encoding C-terminal half of the Neonothopanus nambi hispidin synthase nnHispS codon-optimised for expression in Nicotiana benthamiana

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   C-part of fungal hispidin synthase, nnHispS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pX021

    Plasmid

    #167147

    Purpose

    MoClo-compatible Level 0-SP promoterless vector encoding N-terminal half of the Neonothopanus nambi hispidin synthase nnHispS codon-optimised for expression in Nicotiana benthamiana

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    N-part of fungal hispidin synthase, nnHispS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSMT3-FBF2 SS/Y

    Plasmid

    #166973

    Purpose

    Expresses FBF-2 with SS/Y mutations in repeat 5 in E. coli for purification and crystallization studies

    Depositor

    Traci Hall

    Article

    Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1

    Insert

    FBF-2 (fbf-2 Nematode)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    FBF-2 PUM RNA binding domain; amino acids 164-575…

    Promoter

    T7

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSMT3-FBF2 AS/Y

    Plasmid

    #166974

    Purpose

    Expresses FBF-2 with AS/Y mutations in repeat 5 in E. coli for purification and crystallization studies

    Depositor

    Traci Hall

    Article

    Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1

    Insert

    FBF-2 (fbf-2 Nematode)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    FBF-2 PUM RNA binding domain; amino acids 164-575…

    Promoter

    T7

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LB001: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::Sp/xCas9 TRE#2 gRNA

    Plasmid

    #131757

    Purpose

    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven Sp/xCas9 TRE#2 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#2 gRNA expression

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    TRE#2 Sp/xCas9 gRNA
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Oct. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LA901: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::Sp/xCas9 TRE#1 gRNA

    Plasmid

    #131756

    Purpose

    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven Sp/xCas9 TRE#1 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#1 gRNA expression

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    TRE#1 Sp/xCas9 gRNA
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Oct. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. JV201: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 TRE#1 gRNA

    Plasmid

    #131755

    Purpose

    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven SaCas9 TRE#1 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#1 gRNA expression

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    TRE#1 SaCas9 gRNA
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Oct. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LB101: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 TRE#2 gRNA

    Plasmid

    #131758

    Purpose

    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven SaCas9 TRE#2 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#2 gRNA expression

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    TRE#2 SaCas9 gRNA
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pETM33_Nsp3c_SUD-C

    Plasmid

    #156471

    Purpose

    Bacterial expression of Sars-CoV2 Nsp3c_SUD-C protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp3c_SUD-C (ORF1ab Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. KA901: pMVP (L3-L2) HA tag + pA; EF1a::eGFP-P2A-TETa-pA

    Plasmid

    #121804

    Purpose

    pMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven GFP-P2A-TETa

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    HA epitope tag-polyA + EF1a::eGFP-P2A-TETa-polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. KA701: pMVP (L3-L2) HA tag + pA; RIP::eGFP-P2A-TETa-pA

    Plasmid

    #121808

    Purpose

    pMVP L3-L2 entry plasmid, contains HA tag-polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream RIP-driven GFP-P2A-TETa

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    HA epitope tag-polyA + RIP::eGFP-P2A-TETa-polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. KB701: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; CMV promoter

    Plasmid

    #121818

    Purpose

    pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and CMV promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge