We narrowed to 20,524 results for: nans

1. pLenti CRISPR sgMTHFD2_1

   Plasmid

   #106313

   Purpose

   Express Cas9 and sgRNA targeting MTHFD2

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Insert

   sgRNA targeting MTHFD2 (MTHFD2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAFW-Ago1

   Plasmid

   #50553

   Purpose

   Expression of FLAG tagged Ago1 in S2 cell

   Depositor

   Yukihide Tomari

   Article

   Kawamata et al Nat Struct Mol Biol. 2009 Sep;16(9):953-

   Insert

   Argonaute1 (AGO1 Fly)
   

   Use

   Tags

   FLAG

   Expression

   Insect

   Mutation

   Promoter

   Actin5C

   Available since

   Feb. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcdna DSG-2 Tailless

   Plasmid

   #89466

   Purpose

   DSG-2 tailless control

   Depositor

   Daniel Conway

   Article

   Baddam et al Cells. 2018 Jun 26;7(7). pii: cells70700

   Insert

   desmoglein-2 (DSG2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT7-7 asyn S129A

   Plasmid

   #36048

   Depositor

   Hilal Lashuel

   Article

   Paleologou et al J Biol Chem. 2008 Jun 13;283(24):16895-9

   Insert

   alpha-synuclein S129A (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S129A

   Promoter

   T7

   Available since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. FgH1tUTG_huBim_Ex3

   Plasmid

   #85532

   Purpose

   Inducible expression of guide RNA (huBim_Ex3) with fluorescent GFP reporter

   Depositor

   Marco Herold

   Article

   Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

   Insert

   hu Bim
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1t

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.2 mir-1-1 reporter hsa-mir-122

   Plasmid

   #46673

   Depositor

   David Bartel

   Article

   Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

   Insert

   hsa-mir-122 (MIR122 Human)
   

   Use

   RNAi

   Tags

   V5 and hsa-mir-1-1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. YFP Rab4DN

   Plasmid

   #99019

   Purpose

   Localization and visualization of the dominant negative form of Rab4

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

   Insert

   Rab4 DN (Rab4 Fly, Synthetic)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   S22N Dominant negative

   Promoter

   CMV

   Available since

   June 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS406-PHO5-GFP-OSBP PH domain

   Plasmid

   #58840

   Purpose

   Expresses GFP-Tagged OSBP PH domain in yeast

   Depositor

   Sean Munro, Tim Levine

   Article

   Levine et al Curr Biol. 1998 Jun 18;8(13):729-39.

   Insert

   Oxysterol-binding Protein PH domain (OSBP Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   PHO5

   Available since

   Jan. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pRSET-pr-mEos3.1 (mEosFP3.1-A69T)

   Plasmid

   #99221

   Purpose

   Bacterial expression of the primed conversion capable protein pr-mEos3.1 (mEos3.1-A69T).

   Depositor

   Periklis Pantazis

   Article

   Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

   Insert

   pr-mEos3.1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A69T for primed conversion applications

   Promoter

   T7

   Available since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Ef1a-DO-ChETA-TdTomato-WPRE-pA

    Plasmid

    #37756

    Purpose

    Expresses ChETA-TdTomato in cells lacking Cre (Cre-Off), for optogenetic excitation

    Depositor

    Bernardo Sabatini

    Article

    Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

    Insert

    ChETA-TdTomato
    

    Use

    AAV and Cre/Lox; Cre-off

    Tags

    TdTomato

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-CaMKIIa-Mac 3.0-EYFP

    Plasmid

    #35515

    Purpose

    Lentiviral expression of CaMKIIa-driven Mac 3.0 for optical inhibition.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    eMac 3.0-EYFP
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Trafficking signal, ER export

    Promoter

    CaMKIIa

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmEos2-C1-centrin-1

    Plasmid

    #72588

    Purpose

    mEos2 photoconvertible fluorescent protein fused to the N-terminus of centrin-1

    Depositor

    Michel Bornens

    Article

    Sillibourne et al Cytoskeleton (Hoboken). 2011 Nov;68(11):

    Insert

    centrin 1 (CETN1 Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKLV2-U6gRNA5(Tsc2-g1)-PGKpuroBFP-W

    Plasmid

    #105039

    Purpose

    Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Tsc2 (Tsc2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSP3-bio

    Plasmid

    #47731

    Purpose

    Expresses enzymatically monobiotinylated full-length MSP3 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised MSP3
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSP2-bio

    Plasmid

    #47710

    Purpose

    Expresses enzymatically monobiotinylated full-length MSP2 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised MSP2
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. DCT.VV.Orf106

    Plasmid

    #12794

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jon Yewdell, Jack Bennink

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf106
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pAD-CMV-STAT5B-DN-CMV-GFP

    Plasmid

    #83262

    Purpose

    Adenoviral expression of dominant negative STAT5B with co-expression of GFP

    Depositor

    Andrew Brooks

    Article

    Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

    Inserts

    Stat5b (Stat5b Mouse)
    eGFP
    

    Use

    Adenoviral

    Tags

    FLAG

    Expression

    Mutation

    dominant negative (Y699F)

    Promoter

    CMV

    Available since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. FUW-ubiquitin-SV40-RFP

    Plasmid

    #65448

    Purpose

    Expression of RFP in mammalian cells. Control vector for FUW-Ephrin-RFP vectors

    Depositor

    Eduard Batlle

    Article

    Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

    Insert

    SV40-RFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBQ

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

19. EYFP-IKB

    Plasmid

    #111194

    Purpose

    fluorescent fusion protein

    Depositor

    Johannes A. Schmid

    Article

    Schmid et al J Biol Chem. 2000 Jun 2;275(22):17035-42

    Insert

    IKB-alpha
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBSFI-myr-akt-S473A

    Plasmid

    #49190

    Purpose

    cDNA of Akt1 with myristyolation signal and point mutation from S to A at position 473 in cloning vector

    Depositor

    Peter Vogt

    Article

    Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Adaptor plasmid

    Tags

    HA tag and myristolation signal

    Expression

    Mutation

    S473A

    Promoter

    T7

    Available since

    Dec. 13, 2013

    Availability

    Academic Institutions and Nonprofits only