We narrowed to 22,778 results for: Sis

1. pAB078d7

   Plasmid

   #79205

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (off-target) luxAB; Ppro1 434cI-SH2ABL1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PlacZ-opt (off-target); Ppro1

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAB085e

   Plasmid

   #79212

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (OR1) luxAB; Ptet 434cI-TnTBR3-F7
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PlacZ-opt (OR1); Ptet

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAC-BETA

   Plasmid

   #53272

   Purpose

   Contains crtE, crtB, crtI, and crtY carotenoid pathway genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coli

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Plant Cell. 1996 Sep;8(9):1613-26.

   Insert

   crtE, crtY, crtI, crtB
   

   Use

   Low copy number bacterial cloning vector

   Tags

   Expression

   Mutation

   Promoter

   endogenous promoters

   Available Since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-SpCas9(D10A)-T2A-EGFP

   Plasmid

   #221230

   Purpose

   Express nCas9 nickase (D10) with GFP reporter

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   SpCas9(D10A)-T2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBAD_sfGFP3TCG4TAG

   Plasmid

   #174518

   Purpose

   Recoded sfGFP reporter with the codons TCG and TAG inserted at positions 3 and 4, respectively.

   Depositor

   Jason W Chin

   Article

   Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

   Insert

   sfGFP
   

   Use

   Tags

   6 x His

   Expression

   Bacterial

   Mutation

   Recoded. TCG and TAG codons were introduced in po…

   Promoter

   araBAD

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-TadA8e-PcrA M6

   Plasmid

   #221229

   Purpose

   Express HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase.

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   TadA8e-PcrA M6
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-Anillin

   Plasmid

   #68027

   Purpose

   Full length Anillin rendered RNAi-resistant) in mammalian expression vector pEGFP-C1

   Depositor

   Michael Glotzer

   Article

   Piekny et al Curr Biol. 2008 Jan 8;18(1):30-6. Epub 2

   Insert

   Anillin (ANLN Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   silent mutations at 798 5'-TGCC TCTTTGAATAAA…

   Promoter

   CMV

   Available Since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1D/mEGFP + CrPV IGR IRES-nLuc-3XFLAG-CL1PEST

   Plasmid

   #127332

   Purpose

   Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CrPV IRES

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG and CL1/PEST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC57-attB2-SA(2)-CRY2-mCherry

   Plasmid

   #160441

   Purpose

   To create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 2.

   Depositor

   Nicholas Tolwinski

   Article

   Bunnag et al J Mol Biol. 2020 May 1;432(10):3159-3176

   Insert

   Cryptochrome 2 (CRY2 Mustard Weed)
   

   Use

   Tags

   mCherry

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-SpCas9(H840A)-T2A-EGFP

    Plasmid

    #221231

    Purpose

    Express nCas9 nickase (H840A) with GFP reporter

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    SpCas9(H840A)-T2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H840A

    Promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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