We narrowed to 72,012 results for: Eras
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Plasmid#163656PurposeExpresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail and TMD from ST6GAL1, and luminal domain from TFR in mammalian cellsDepositorInsertTagsGFPExpressionMammalianMutationThis chimera contains the cytosolic tail from ST,…Available SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only
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FFS-GFP
Plasmid#163659PurposeExpresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail and TMD from TFR, and luminal domain from ST6GAL1 in mammalian cellsDepositorInsertTagsGFPExpressionMammalianMutationThis chimera contains the cytosolic tail from TFR…Available SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
SFS-GFP
Plasmid#163655PurposeExpresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail from ST6GAL1, TMD from TFR and luminal domain from ST6GAL1 in mammalian cellsDepositorInsertTagsGFPExpressionMammalianMutationThis chimera contains the cytosolic tail from ST,…Available SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
SFF-GFP
Plasmid#163657PurposeExpresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail from ST6GAL1, and TMD and luminal domain from TFR in mammalian cellsDepositorInsertTagsGFPExpressionMammalianMutationThis chimera contains the cytosolic tail from ST,…Available SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-Polß(WT)--IRES-hyg
Plasmid#128667Purposemammalian expression of Flag-Polß(WT) protein with hygromycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagsFlagExpressionMammalianMutationWild typePromoterCMVAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMiR-E6AP-3'UTR-miR-375-mut
Plasmid#53695PurposeLuciferase reporter assay for E6AP 3'UTR with point mutation on miR-375 binding siteDepositorInsertUBE3A 3'UTR (UBE3A Human)
UseLuciferaseMutationMutation on miR-375 binding site on E6AP 3'U…Available SinceJuly 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMiR-CIP2A(partial)
Plasmid#53705PurposeLuciferase reporter assay for microRNA binding on CIP2A coding sequenceDepositorInsertCIP2A (CIP2A Human)
UseLuciferaseAvailable SinceAug. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-3exo-Tetra-com-CLCN5-sp-g1
Plasmid#176236PurposePlasmid for expressing a fusion protein of spCas9 and the 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.DepositorInsertspCas9 and polymerase exo domain fusion (CLCN5 Human)
ExpressionBacterialAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-5Exo-tetra-com-hCLCN5-sp-g1
Plasmid#176237PurposePlasmid for expressing a fusion protein of spCas9 and the 5’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.DepositorInsertspCas9 and polymerase exo domain fusion (CLCN5 Human)
ExpressionBacterialAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-Exo-tetra-com-hCLCN5-sp-g1
Plasmid#176238PurposePlasmid for expressing a fusion protein of spCas9 and the 5’ and 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.DepositorInsertspCas9 and polymerase exo domain fusion (CLCN5 Human)
ExpressionBacterialAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
NSS-GFP
Plasmid#163661PurposeExpresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail from TNF and TMD and luminal domain from ST6GAL1 in mammalian cellsDepositorInsertTagsGFPExpressionMammalianMutationThis chimera contains the cytosolic tail from TNF…Available SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
SNN-GFP
Plasmid#163664PurposeExpresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail from ST6GAL1, and TMD and luminal domain from TNF in mammalian cellsDepositorInsertTagsGFPExpressionMammalianMutationThis chimera contains the cytosolic tail from ST,…Available SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
NNS-GFP
Plasmid#163666PurposeExpresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail and TMD from TNF, and luminal domain from ST6GAL1 in mammalian cellsDepositorInsertTagsGFPExpressionMammalianMutationThis chimera contains the cytosolic tail from TNF…Available SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCT-CMV-copGFP-Polß(WT)-puro
Plasmid#128665Purposemammalian expression of copGFP-Polß(WT) protein with puromycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagscopGFPExpressionMammalianMutationWild typePromoterCMVAvailable SinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-SPRED1-3'UTR (mut - miR-21 site1)
Plasmid#62580PurposeTranslational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The upstream miR-21 (site 1) binding site was mutated.DepositorInsertSPRED1 (SPRED1 Human)
UseLuciferaseMutationmiR-21 binding site 1 mutant (TAAGCTA --> TAGA…Available SinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-SPRED1-3'UTR (mut - miR-21 site 2)
Plasmid#62581PurposeTranslational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The downstream miR-21 binding site (site 2) was mutated.DepositorInsertSPRED1 (SPRED1 Human)
UseLuciferaseMutationmiR-21 binding site 2 mutant (TAAGCTA --> TAGA…Available SinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-Venus-Fluc (puro)
Plasmid#140328PurposeLentivirus for expression of Venus-Fluc (firefly luciferase) fusion proteinDepositorInsertVenus-Firefly Luciferase (luc2)
UseLentiviralPromoterhuman PGK promoterAvailable SinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSynSRE-T-Luc
Plasmid#60444PurposeUses the hamster HMG-CoA synthase promoter sequence (-324/-225) and minimal HMG-CoA sythnase TATA box (-28/+39) to study SREBP-dependent transcriptional activation.DepositorInsertHMG-CoA synthase promoter (-324/-225)
UseLuciferaseTagsLuciferaseExpressionMammalianPromoterHMG-CoA synthase (-324/-225) & TATAA (-28/+39)Available SinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαi3-LgB91
Plasmid#134359PurposeNanoluc complementation assay. Expression of Gαi3 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi3. Addition of the HA epitope at N terminus of Gαi3.DepositorAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαi2-LgB91
Plasmid#134358PurposeNanoluc complementation assay. Expression of Gαi2 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi2. Addition of the HA epitope at N terminus of Gαi2.DepositorAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only