Addgene

Showing: 1 - 20 of 63 results

Results per page:

Sort results by:

EC1000

Bacterial Strain

#71852

Purpose

RepA+ MC1000, KmR , carrying a single copy of the pWV01 repA gene in the glgB gene; host for pOR128-based plasmids

Depositor

Todd Klaenhammer, Jan Kok

Article

Leenhouts et al Mol Gen Genet. 1996 Nov 27;253(1-2):217-

Bacterial Resistance

Kanamycin

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only
Strain SIJ_488

Bacterial Strain

#68246

Purpose

E. coli K-12 MG1655 with genomically integrated arabinose inducible lambda Red recombineering genes, rhamnose inducible flippase recombinase and m-toluic acid inducible I-SceI.

Depositor

Alex Nielsen

Article

Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103

Bacterial Resistance

None

Available since

Dec. 8, 2015

Availability

Academic Institutions and Nonprofits only
CYN2/150 (E. coli Entner-Doudoroff v11)

Bacterial Strain

#71420

Purpose

This E. coli MG1655 strain has a genome-integrated genetic module containing a rationally engineered 5-enzyme Entner-Doudoroff pathway with an improved NADPH regeneration rate. Module is Cm resistant

Depositor

Howard Salis

Article

Ng et al Metab Eng. 2015 May;29:86-96. doi: 10.10

Bacterial Resistance

Chloramphenicol

Available since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only
DHIP1

Bacterial Strain

#226851

Purpose

Expresses HIP1 methyltransferase to methylate plasmids for delivery to cyanobacteria

Depositor

David Nielsen

Article

Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

Bacterial Resistance

Streptomycin

Species

Escherichia coli

Available since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only
AdEasier-1 cells (strain)

Bacterial Strain

#16399

Depositor

Bert Vogelstein

Article

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Bacterial Resistance

Ampicillin and streptomycin

Species

Escherichia coli

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
MutaT7

Bacterial Strain

#156460

Purpose

An E. coli strain that expresses MutaT7 for mutagenizing genes downstream from a T7 promoter.

Depositor

Matthew D. Shoulders

Article

Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

Bacterial Resistance

Streptomycin

Species

Rattus norvegicus

Available since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only
E. coli BL21 (DE3) ΔEntD

Bacterial Strain

#192874

Purpose

Deletion of native E. coli EntD, a 4'-PPTase required for native enterobactin synthesis. Allows expression of NRPS carrier proteins that do not harbor a phosphopantetheinyl group.

Depositor

Christian Chalut, Christophe Guilhot

Article

Chalut et al Proc Natl Acad Sci U S A. 2006 May 30;10

Bacterial Resistance

None

Species

Escherichia coli

Available since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only
drApo1–T7

Bacterial Strain

#156461

Purpose

An E. coli strain that expresses an inactive variant of MutaT7. Used as a control strain for mutagenesis experiments with MutaT7.

Depositor

Matthew D. Shoulders

Article

Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

Bacterial Resistance

Streptomycin

Species

Rattus norvegicus

Available since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only
MK01

Bacterial Strain

#195090

Purpose

Improved E. coli strain created by knocking out the endogenous lacI gene with chloramphenicol acetyltransferase (cat) selection cassette in the parent strain BW27783 that overcome the all-or-none response of araBAD promoter (PBAD).

Depositor

Sander Tans

Article

Kogenaru et al J Biol Eng. 2014 Jan 2;8(1):2. doi: 10.1

Bacterial Resistance

Chloramphenicol

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only
BW-Para

Bacterial Strain

#172602

Purpose

The BW-Para E. coli strain is used to screen the functions of chimeric AraC/XylS transcription activators using beta-galactosidase assays.

Depositor

Liskin Swint-Kruse

Article

Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

Bacterial Resistance

None

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only
Escherichia coli DH10B-MOB

Bacterial Strain

#229395

Purpose

Escherichia coli DH10B DAP-auxotrophic strain containing an integrated RK2/RP4 mobilization vector to conjugate plasmids with an IncP origin of transfer (modified pTA-Mob 2.0, AddGene Plasmid #149662)

Depositor

Michael Springer

Article

Soltysiak et al ACS Synthetic Biology. 2025. doi: 10.102

Bacterial Resistance

60 ug/ml of dap

Species

Escherichia coli

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only
BLIM cells

Bacterial Strain

#35609

Depositor

Kathleen Matthews

Article

Wycuff et al Anal Biochem. 2000 Jan 1. 277(1):67-73.

Bacterial Resistance

None

Available since

June 21, 2012

Availability

Academic Institutions and Nonprofits only
HB101 endA::frt

Bacterial Strain

#45473

Depositor

Michele Swanson

Article

Bryan et al Mol Microbiol. 2011 Apr;80(1):231-47. do

Bacterial Resistance

Streptomycin

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
MG1655 ΔendA ΔrecA (DE3)

Bacterial Strain

#37854

Depositor

Kristala Prather

Article

Tseng et al Microb Cell Fact. 2010 Nov 27;9:96.

Bacterial Resistance

None

Available since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only
MM39

Bacterial Strain

#42894

Depositor

Bryan Berger

Article

Su et al J Biol Chem. 2012 Jul 22.

Bacterial Resistance

Streptomycin

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
EcNR2 Strain

Bacterial Strain

#26931

Depositor

George Church

Article

Wang et al Nature. 2009 Aug 13. 460(7257):894-8.

Bacterial Resistance

Chloramphenicol and ampicillin

Available since

Jan. 28, 2011

Availability

Academic Institutions and Nonprofits only
HB101 endA::frt lambda pir

Bacterial Strain

#45472

Depositor

Michele Swanson

Article

Bryan et al Mol Microbiol. 2011 Apr;80(1):231-47. do

Bacterial Resistance

Streptomycin

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
JS200 strain

Bacterial Strain

#11794

Depositor

Lawrence Loeb

Article

Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 1

Bacterial Resistance

None

Available since

June 20, 2007

Availability

Academic Institutions and Nonprofits only
HL3721

Bacterial Strain

#52950

Purpose

HL716 + ΔcsrB + ΔcsrC + ΔglgCAP + ΔpgaABCD

Depositor

Han Lim

Article

Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

Bacterial Resistance

None

Species

Escherichia coli

Available since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only
DH10B-M.Osp807II

Bacterial Strain

#114267

Purpose

The strain expresses M.Osp807II methylase and zeocin selection marker from the arsB locus.

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Bacterial Resistance

Bleocin (zeocin)

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

Showing: 1 - 20 of 63 results

Results per page:

Sort results by:

EC1000

Strain SIJ_488

CYN2/150 (E. coli Entner-Doudoroff v11)

DHIP1

AdEasier-1 cells (strain)

MutaT7

E. coli BL21 (DE3) ΔEntD

drApo1–T7

MK01

BW-Para

Escherichia coli DH10B-MOB

BLIM cells

HB101 endA::frt

MG1655 ΔendA ΔrecA (DE3)

MM39

EcNR2 Strain

HB101 endA::frt lambda pir

JS200 strain

HL3721

DH10B-M.Osp807II

Help Center

Deposit Plasmids

General Reagents

Viral Service

DNA Service

Plasmid Collections

	More than 20 requests
	More than 50 requests
	More than 100 requests