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Showing: 1 - 20 of 29 results

  1. Sybody Generation Toolbox

    Plasmid Kit
    #1000000160
    Purpose
    The Seeger Lab protocol to select synthetic nanobodies – called sybodies – against (membrane) protein targets. This collection contains all plasmids necessary for the sybody generation.
    Depositor
    Markus Seeger
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only

  2. Target Accelerator Lung Cancer Mutant Collection

    Plasmid Kit
    #1000000104
    Purpose
    Panel of lung adenocarcinoma alleles that have undergone basic, functional screening; full-length human clones (180 mutant and 45 corresponding wild-type) and 82 wild-type experssion controls
    Depositor
    Jesse Boehm
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only

  3. Target Accelerator Genetic Pathway Reference Set

    Plasmid Kit
    #1000000105
    Purpose
    Collection of genes and mutations for reference pathway signatures and phenotypes to compare with those derived from cancer alleles; represent over 25 cellular signaling pathways and processes
    Depositor
    Jesse Boehm
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only

  4. Target Accelerator Pan-Cancer Mutant Collection

    Plasmid Kit
    #1000000103
    Purpose
    Panel of cancer alleles that have undergone basic, functional screening; full-length, human clones (454 mutant and 177 corresponding wild-type) in the Gateway cloning system
    Depositor
    Jesse Boehm
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only

  6. GoldenBraid 2.0 Kit

    Plasmid Kit
    #1000000076
    Purpose
    Modular cloning of multigenic DNA constructs in Plant Synthetic Biology applications. 95 GoldenBraid cloning compatible plasmids, containing standard basic parts and transcriptional units
    Depositor
    Diego Orzaez
    Available since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only

  7. Multiple Lentiviral Expression (MuLE) system

    Plasmid Kit
    #1000000060
    Purpose
    Multiple Lentiviral Expression (MuLE): modular system used to construct complex lentiviruses for modification of mammalian cells with a single viral infection.
    Depositor
    Ian Frew
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only

  8. Cancer Pathways ORFs Kit

    Plasmid Kit
    #1000000072
    Purpose
    A set of vectors containing cDNAs for studying oncogenic signaling pathways. cDNAs are either wild-type, constitutively active mutants, or dominant negative mutants.
    Depositor
    David Sabatini, Kris Wood
    Available since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only

  9. biGBac Kit

    Plasmid Kit
    #1000000088
    Purpose
    Generation of baculoviral transfer vectors coding up to 25 protein complex subunits in two Gibson assembly steps. Multisubunit protein complexes can be generated in baculovirus-infected insect cells.
    Depositor
    Jan-Michael Peters
    Available since
    Oct. 25, 2016
    Availability
    Academic Institutions and Nonprofits only

  10. Minimal Antibiotic Resistance Platform (ARP)

    Plasmid Kit
    #1000000143
    Purpose
    Used for the general dereplication of antibiotics in natural product extracts. Plasmids constitutively express individual resistance genes that target the most commonly found antibiotics.
    Depositor
    Gerard Wright
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only

  11. LIC TAL Effector Assembly Kit

    Plasmid Kit
    #1000000023
    Purpose
    LIC (ligation-independent cloning) TALE repeat unit library of 2-mer fragments for assembly in an expression ready TALEN construct in 2 hierarchical assembly steps. Semi‐automated TALE generation
    Depositor
    Veit Hornung
    Available since
    Jan. 2, 2013
    Availability
    Academic Institutions and Nonprofits only

  12. pGCS vector kit

    Plasmid Kit
    #1000000107
    Purpose
    A series of pCS2+ based Gateway vectors with N- or C-terminal tags (eg Myc, HA, Flag, GST and eGFP), which allow the generation of tagged proteins in a variety of vertebrate organisms.
    Depositor
    Hai-Ning Du
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only

  13. Yeast GoldenBraid Cloning System and Toolkit

    Plasmid Kit
    #1000000138
    Purpose
    Modular cloning system for generation of high expression transcriptional units; Integrates in yeast (S. cerevisiae) genome at two different loci supporting high and stable transgene expression
    Depositor
    Elena Caro Bernat
    Available since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only

  14. C. elegans miniMos transgenesis kit

    Plasmid Kit
    #1000000031
    Purpose
    29 plasmid kit contains reagents for generating transgenic nematodes, including C. elegans and C. briggsae, using miniMos transposon. Selection with unc-119 or NeoR, PuroR.
    Depositor
    Erik Jorgensen
    Available since
    July 30, 2013
    Availability
    Academic Institutions and Nonprofits only

  15. MoBiFC

    Plasmid Kit
    #1000000222
    Purpose
    The modular bimolecular fluorescence complementation (MoBiFC) toolkit allows for the analysis of protein-protein interactions in plants.
    Depositor
    Benjamin Field
    Available since
    July 26, 2024
    Availability
    Academic Institutions and Nonprofits only

  16. cpFRET

    Plasmid Kit
    #1000000021
    Purpose
    circular permutation-based fluorescence resonance energy transfer (cpFRET) biosensor toolkit: generate and optimize genetically encoded, unimolecular FRET sensors for ratiometric measurements
    Depositor
    Olivier Pertz
    Available since
    July 5, 2013
    Availability
    Academic Institutions and Nonprofits only

  17. FREQ-Seq Kit

    Plasmid Kit
    #1000000032
    Purpose
    For construction of barcoded, locus-specific libraries compatible with Illumina next generation sequencing. Allows for direct quantitative allele detection. 48 plasmid adaptors (barcoded M13F primer)
    Depositor
    Christopher Marx
    Available since
    May 23, 2013
    Availability
    Academic Institutions and Nonprofits only

  18. MetClo DNA Assembly Kit

    Plasmid Kit
    #1000000153
    Purpose
    MetClo DNA Assembly Kit allows modular, hierarchical assembly of DNA fragments using IIS restriction enzyme. Includes 3 E.coli strains for BsaI, BpiI and LguI-based MetClo assembly.
    Depositor
    Christopher A. O'Callaghan
    Available since
    April 22, 2019
    Availability
    Academic Institutions and Nonprofits only

  20. Barcoded self-reporting transposon calling card collection

    Plasmid Kit
    #1000000213
    Purpose
    'Calling Cards' technology using self-reporting transposons (SRTs) enables the identification of DNA-protein interactions through RNA sequencing.
    Depositor
    Rob Mitra
    Available since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 29 results