We narrowed to 11,754 results for: pcDNA

1. pcDNA3.1-HA-GTSE1-S91A

   Plasmid

   #224721

   Purpose

   For expression of HA-tagged GTSE1 protein with S91A mutation

   Depositor

   Michele Pagano

   Article

   García-Vázquez et al bioRxiv 2024.06.26.600797

   Insert

   GTSE1 (GTSE1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Serine 91 to Alanine

   Promoter

   CMV

   Available since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-HA-GTSE1-S157A

   Plasmid

   #224722

   Purpose

   For expression of HA-tagged GTSE1 protein with S157A mutation

   Depositor

   Michele Pagano

   Article

   García-Vázquez et al bioRxiv 2024.06.26.600797

   Insert

   GTSE1 (GTSE1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Serine 157 to Alanine

   Promoter

   CMV

   Available since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1/V5-His-mPer1

   Plasmid

   #219421

   Purpose

   Expresses 3873 bp of mPer1 coding sequence in E. coli.

   Depositor

   Aziz Sancar

   Article

   Cao et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

   Insert

   mPer1 (Per1 Mouse)
   

   Use

   Tags

   His and V5

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-Nuc-deltaCKAR

   Plasmid

   #222430

   Purpose

   FRET-based reporter for monitoring deltaPKC activity at the nucleus in cells.

   Depositor

   Alexandra Newton

   Article

   Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

   Insert

   Nucleus delta-selective C kinase activity reporter
   

   Use

   Tags

   CFP and YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3 Mito(OM)-deltaCKAR

   Plasmid

   #222431

   Purpose

   FRET-based reporter for monitoring deltaPKC activity at the mitochondrial outer membrane in cells.

   Depositor

   Alexandra Newton

   Article

   Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

   Insert

   Mitochondrial outer membrane delta-selective C kinase activity reporter
   

   Use

   Tags

   CFP and YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-Golgi-deltaCKAR

   Plasmid

   #222428

   Purpose

   FRET-based reporter for monitoring deltaPKC activity at the Golgi in cells.

   Depositor

   Alexandra Newton

   Article

   Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

   Insert

   Golgi delta-selective C kinase activity reporter
   

   Use

   Tags

   CFP and YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1_SM-FLuc0-malat1

   Plasmid

   #210580

   Purpose

   Expresses SM(FLAG)-nonoptimal Firefly - 3'UTR malat1 triple helix

   Depositor

   Olivia Rissland

   Article

   Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

   Insert

   Spaghetti monster (FLAG)-nonoptimal FLuc - 3'UTR malat1 triple helix
   

   Use

   Luciferase

   Tags

   Spaghetti monster (FLAG)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1_SM-FLuc100-malat1

   Plasmid

   #210581

   Purpose

   Expresses SM(FLAG)-optimal Firefly luciferase - 3'UTR malat1 triple helix

   Depositor

   Olivia Rissland

   Article

   Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

   Insert

   Spaghetti monster (FLAG)-optimal FLuc - 3'UTR malat1 triple helix
   

   Use

   Luciferase

   Tags

   Spaghetti monster (FLAG)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-FLAG3-drAgo2D-A

   Plasmid

   #221734

   Purpose

   Expression of zebrafish Ago2 with D683A mutation and N-terminal 3xFLAG tag in mammalian cells.

   Depositor

   David Bartel

   Article

   Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

   Insert

   drAgo2D-A (ago2 Zebrafish)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D683A in active site

   Promoter

   CMV

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-FLAG3-drAgo2DY-EF

    Plasmid

    #221733

    Purpose

    Expression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in mammalian cells.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    drAgo2DY-EF (ago2 Zebrafish)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D651E and Y680F in active site

    Promoter

    CMV

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-FLAG3-drAgo2Y-F

    Plasmid

    #221732

    Purpose

    Expression of zebrafish Ago2 with Y680F mutation and N-terminal 3xFLAG tag in mammalian cells.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    drAgo2Y-F (ago2 Zebrafish)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Y680F in active site

    Promoter

    CMV

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-FLAG3-drAgo2D-E

    Plasmid

    #221731

    Purpose

    Expression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in mammalian cells.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    drAgo2D-E (ago2 Zebrafish)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D651E in active site

    Promoter

    CMV

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-FLAG3-drAgo2

    Plasmid

    #221730

    Purpose

    Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in mammalian cells.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    drAgo2 (ago2 Zebrafish)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3puro-EGFP-GAPDH-CC

    Plasmid

    #225476

    Purpose

    Express mEGFP-GAPDH-CC fusion

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    GAPDH fused with CC2 domain of ROCK2 (GAPDH Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCDNA3-Regnase-1 D141N

    Plasmid

    #222654

    Purpose

    Mammalian expression vector to express mouse Regnase-1 D141N mutant

    Depositor

    Silvia Monticelli

    Article

    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

    Insert

    Zc3h12a (Zc3h12a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mutated aspartic acid 141 to asparagine to abroga…

    Promoter

    CMV

    Available since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(+) M-Ras(G22C)

    Plasmid

    #224288

    Purpose

    Cell transfection and expression of M-Ras (G22C)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    M-Ras (MRAS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G22C

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1(+) Rheb(R15C)

    Plasmid

    #224290

    Purpose

    Cell transfection and expression of Rheb (R15C)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    Rheb (RHEB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R15C

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1(+) R-Ras1(G38C)

    Plasmid

    #224286

    Purpose

    Cell transfection and expression of R-Ras1 (G38C)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    R-Ras1 (RRAS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G38C

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1:mTRESK(S116C)

    Plasmid

    #224772

    Purpose

    In vitro transcription for Oocyte expression of mTRESK(S116C)

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily member 18 (Kcnk18 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    S116C

    Promoter

    T7

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1(+) EGFP-Rap1A(WT)

    Plasmid

    #224276

    Purpose

    Cell transfection and expression of Rap1A

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    Rap1A (RAP1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge