We narrowed to 28,023 results for: STI;
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Plasmid#71358Purposecsd6, construct:13-330, pET28b(+) N-tag, mutation:W158ADepositorInsertHP0518 (HP0518 changed Tryptophan 158 to Alanine)
TagsHistidine tagExpressionBacterialPromoterT7Available SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd6_13-330_Y132A/pET28b(+) N-tag
Plasmid#71355Purposecsd6, construct:13-330, pET28b(+) N-tag, mutation:Y132ADepositorInsertHP0518 (HP0518 changed Tyrosine 132 to Alanine)
TagsHistidine tagExpressionBacterialPromoterT7Available SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBullet-cyt-n
Plasmid#53065Purposedestination vector with cytosol marker (ECFP) for tagged fluorescent protein (N-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsECFP and EYFPAvailable SinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBullet-er-n
Plasmid#53067Purposedestination vector with WAK2 (29 aa)-ECFP- ER marker (HDEL) for tagged fluorescent protein (N-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsECFP and EYFPAvailable SinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBullet-tgn-n
Plasmid#53071Purposedestination vector with ECFP-trans golgi network marker (VTI12) for tagged fluorescent protein (N-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
Villinpromoter-blue-FlpOERT2
Plasmid#67278Purpose4hydroxytamoxifen inducible FlpO recombinase controlled by intestine specific promoter (Villin). FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon contextDepositorInsertVillin-mTQ2-P2A-FlpO-ERT2
TagsmTQ2, linked to FlpO through an P2A ribosomal ski…ExpressionMammalianPromoterVillinAvailable SinceJuly 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDRM166 LKB (Luciferase mKate2 Blast)
Plasmid#183502PurposeExpresses the firefly luciferase gene, the NLS-tagged mKate2 gene, and the blasticidin resistance gene joined by P2A linkers.DepositorInsertsFirefly Luciferase
mKate2
BSD
UseLentiviral and LuciferaseTagsP2A linker and SV40 NLS with GGS linkerExpressionMammalianPromoterMND, MND (off Luc-P2A), and MND (off Luc-P2A-mKat…Available SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EFS-BirAopt-GSQ-RBXN-P2A-BLAST
Plasmid#208048PurposeEnables constitutive expression of BirAopt alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; to perform bioE3; selection with blasticidinDepositorInsertBirAopt
UseLentiviralMutationBirAopt is human-codon-optimized BirA (PMID 15707…PromoterEFSAvailable SinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
Click editor (CE1) - pCMV-T7-PCV2-nCas9-EcKlenow (EMK488)
Plasmid#208942PurposeThe CE1 construct, expressed from CMV or T7 promoters.DepositorInsertPCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); EcKlenow(-exo;D355A/E357A)PromoterCMV and T7Available SinceNov. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
Control click editor - pCMV-T7-PCV2-deadCas9-EcKlenow (EMK500)
Plasmid#208944PurposeA control CE1 construct with catalytically inactivated SpCas9, expressed from CMV or T7 promoters.DepositorInsertPCV2-XTEN-dSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationdSpCas9(D10A/H840A);EcKlenow(-exo;D355A/E357A)PromoterCMV and T7Available SinceNov. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-PolBeta (JO574)
Plasmid#208953PurposeA variant CE1 construct with human DNA polymerase beta, expressed from CMV or T7 promoters.DepositorInsertPCV2-XTEN-nSpCas9-BPNLS-PolBeta-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A)PromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
HR700_HA2TP53_Ex5
Plasmid#228860PurposeBackbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 5DepositorTypeEmpty backboneUseCRISPR and Cre/LoxExpressionMammalianAvailable SinceFeb. 4, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
HR700_HA2TP53_Ex6
Plasmid#228861PurposeBackbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 6DepositorTypeEmpty backboneUseCRISPR and Cre/LoxExpressionMammalianAvailable SinceFeb. 4, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
HR700_HA2TP53_Ex7
Plasmid#228862PurposeBackbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 7DepositorTypeEmpty backboneUseCRISPR and Cre/LoxExpressionMammalianAvailable SinceFeb. 4, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
HR700_HA2TP53_Ex8
Plasmid#228863PurposeBackbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 8DepositorTypeEmpty backboneUseCRISPR and Cre/LoxExpressionMammalianAvailable SinceFeb. 4, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVAX1-BMP2/7 +
Plasmid#137911PurposeConstitutive mammalian co-expression vector for human bone morphogenetic protein 2 and 7 cDNAs (2 transcriptional units, tandem)DepositorAvailable SinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
LV-Sox10MCS5-GFP-EF1a-mCherry
Plasmid#115782PurposeSox10-MCS5-GFP and a constitutive EF1α-mCherry reporter plasmid. Construct generates constitutive red fluorescence and basal promoter cfos conjugated SOXMCS5 enhancer driven GFP expression in a cell.DepositorInsertsmouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter
EF1alpha promoter driven constitutive mCherry
UseLentiviralTagsEF1α promoter fused to mCherry and cfos promoter …PromoterSOX10 Multiple Species Conserved enhancer element…Available SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEF6-Lck-TurboID
Plasmid#159433Purposeexpresses Lck-TurboID in mammalian cellsDepositorInsertsTagsHAExpressionMammalianMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…PromoterEF-1aAvailable SinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMThy1.1_hDDX5_FL
Plasmid#88873Purposeconstitutive expression of DDX5 in mammalian cellsDepositorAvailable SinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOGS539_haPD-1
Plasmid#226716PurposePlasmid enabling yeast-mediated expression and secretion of high affinity PD-1 microbody (haPD-1)DepositorInsertHigh affinity PD-1 microbody
TagsHA tag and Mating factor alpha secretion signalExpressionYeastPromoterpTEF1Available SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only