We narrowed to 4,239 results for: erf

1. pVH321-2-Tier1-PhCMV-NLS-dCas9-3xNLS

   Plasmid

   #169596

   Purpose

   Tier-1 vector encoding PhCMV-driven NLS-dCas9-3xNLS expression (PhCMV-NLS-dCas9-3xNLS-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   dead S.pyogenes Cas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_Blasto

   Plasmid

   #167896

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. NAX_CAGGS_CAS9_m4_KRAB_Puro

   Plasmid

   #167879

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. NAX_CAGGS_CAS9_m4_KRAB_iRFP713

   Plasmid

   #167876

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. NAX_CAGGS_CAS9_m4_KRAB_Hygro

   Plasmid

   #167875

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. NAX_CAGGS_CAS9_m4_KRAB_EYFP

   Plasmid

   #167874

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. NAX_CAGGS_CAS9_m4_KRAB_Blasto

   Plasmid

   #167872

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28-sfGFP-DogTag Loop C

   Plasmid

   #171781

   Purpose

   Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Asp173 and Gly174

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   sfGFP-DogTag Loop C
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   DogTag and linkers inserted between residues Asp1…

   Promoter

   T7

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28-sfGFP-SpyTag003 Loop B

   Plasmid

   #171777

   Purpose

   Expresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Asp102 and Asp103

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   sfGFP-SpyTag003 Loop B
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   SpyTag003 and linkers inserted between residues A…

   Promoter

   T7

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28-sfGFP-SpyTag003 Loop C

    Plasmid

    #171778

    Purpose

    Expresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Asp173 and Gly174

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    sfGFP-SpyTag003 Loop C
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    SpyTag003 and linkers inserted between residues A…

    Promoter

    T7

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSUMO human-cPLA2-C2-domain

    Plasmid

    #170752

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Hirano et al Elife. 2019 May 3;8. pii: 44760. doi: 10

    Insert

    PLA2G4A-C2 domain (PLA2G4A Human)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET30 W96A-GLTP

    Plasmid

    #170734

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Malinina et al Nature. 2004 Aug 26;430(7003):1048-53. d

    Insert

    W96A-GLTP (GLTP Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    W96A

    Promoter

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3_ReBphP-PCM_IRES_sfGFP

    Plasmid

    #166917

    Purpose

    Express ReBphP-PCM and sfGFP

    Depositor

    Andre Stiel

    Article

    Mishra et al Sci Adv. 2020 Jun 12;6(24):eaaz6293. doi

    Inserts

    ReBphP-PCM
    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. DT048A

    Plasmid

    #159409

    Purpose

    Constitutive LuxR expression, controlled under J23101 E.coli promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoter

    Depositor

    Sheref Mansy

    Article

    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

    Insert

    pr.J23101-LuxR-pr.LuxR-PFO
    

    Use

    Synthetic Biology

    Tags

    FLAG/FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    J23101 and pLux promoter

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. DT046A

    Plasmid

    #159407

    Purpose

    Constitutive LuxR expression, controlled under E.coli tet promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoter

    Depositor

    Sheref Mansy

    Article

    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

    Insert

    pr.tet-LuxR-pr.LuxR-PFO
    

    Use

    Synthetic Biology

    Tags

    FLAG/FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    tet and pLux promoter

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJUMP29[dCas9]-1A(lacZ)

    Plasmid

    #126979

    Purpose

    Level 1 vector. With lacZ as alternative cloning reporter.OriV 9 (pBBR322/ROP; medium copy number). Constitutive dCas9 in downstream secondary site.

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Cas9 catalytically dead

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEPOR2KN0078

    Plasmid

    #117645

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for eSaCas9(pEPOR1CB0113), sgRNA{AT1G73460}(pEPOR1CB0099) and sgRNA{AT1G73460}(pEPOR1CB0110) to Protein kinase superfamily protein.

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:eSaCas9(pEPOR1CB0113) ] +[AtU6-26:sgRNA]
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMOD_A0808

    Plasmid

    #91024

    Purpose

    Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Csy4-P2A-AtCas9_dead (D10A + H840A)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, H840A

    Promoter

    EC1.2

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMOD_A0408

    Plasmid

    #91010

    Purpose

    Module A, Promoter: EC1.2, Gene: AtCas9_dead (D10A + H840A), Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9_dead (D10A + H840A)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, H840A

    Promoter

    EC1.2

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Pan-Druggable Cancer Library

    Pooled Library

    #182133

    Purpose

    All-in-one CRISPR/Cas system with 74 individual elements. 63 elements target cancer-related genes with FDA-approved drug treatments, and 3 elements target key medulloblastoma driver genes.

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available since

    Aug. 26, 2022

    Availability

    Academic Institutions and Nonprofits only