We narrowed to 4,239 results for: erf

1. pCB-2

   Plasmid

   #210388

   Purpose

   All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces

   Depositor

   Gilles van Wezel

   Article

   Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021

   Inserts

   dCas9
   CymR
   sgRNA cassette
   

   Use

   CRISPR; Integrative vector for use in streptomyces

   Tags

   Expression

   Mutation

   BsaI-flanked RFP cassette inserted in place of sp…

   Promoter

   SP11 and SP30

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCB-1

   Plasmid

   #210387

   Purpose

   All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces

   Depositor

   Gilles van Wezel

   Article

   Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021

   Inserts

   dCas9
   CymR
   sgRNA cassette
   

   Use

   CRISPR; Integrative vector for use in streptomyces

   Tags

   Expression

   Mutation

   BsaI-flanked RFP cassette inserted in place of sp…

   Promoter

   SP11 and SP30

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-pMecp2-dSaCas9-KRAB-pU6-sgRNA

   Plasmid

   #158988

   Purpose

   Vector E encodes pAAV-pMecp2-dSaCas9-KRAB-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR interference in neurons

   Depositor

   Chung Tin

   Article

   Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

   Insert

   dSaCas9-KRAB
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pMecp2

   Available since

   Aug. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiCRISPRi(v2)-Blast

   Plasmid

   #170068

   Purpose

   Expresses dCas9 repressor KRAB-dCas9-MeCP2 and blasticidin resistance

   Depositor

   Neville Sanjana

   Article

   Morris et al Science. 2023 May 19;380(6646):eadh7699.

   Insert

   KRAB-dCas9-MeCP2
   

   Use

   CRISPR and Lentiviral

   Tags

   2A and 2xHA tag

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP

   Plasmid

   #71237

   Purpose

   Express sgRNA and dCas9-KRAB from 3rd generation lentiviral vector

   Depositor

   Charles Gersbach

   Article

   Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

   Inserts

   humanized dCas9-KRAB T2A GFP
   sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mutation

   D10A and H840A

   Promoter

   Available since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_KanR neo

   Plasmid

   #167901

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. lenti_dCas9-KRAB-MeCP2

   Plasmid

   #122205

   Purpose

   Bipartite dCas9 repressor (dCas9-KRAB-MeCP2) in a UCOE-SFFV-dCas9 lentiviral vector.

   Depositor

   Andrea Califano

   Article

   Tan et al bioRxiv 2021.06.28.449297

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   Lentiviral

   Tags

   KRAB-MeCP2

   Expression

   Mammalian

   Mutation

   Cas9 (D10A, H840A)

   Promoter

   Available since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-dCas9

   Plasmid

   #47106

   Purpose

   Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Flag and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive)

   Promoter

   CMV

   Available since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJMP1339

   Plasmid

   #119271

   Purpose

   for cloning new sgRNAs

   Depositor

   Carol Gross, Oren Rosenberg, Jason Peters

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   dcas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Promoter

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB_tre_dCas9_KRAB

    Plasmid

    #126030

    Purpose

    PiggyBac cargo vector expressing doxycycline inducible dCas9-KRAB fusion

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

    Inserts

    dCas9 fusion with KRAB
    Hygromycin resistance
    

    Use

    CRISPR; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    EF1 alpha and TRE

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLX-TRE-dCas9-KRAB-MeCP2-BSD

    Plasmid

    #140690

    Purpose

    Inducible knockdown of gene expression in human cells for pooled scRNA-seq experiments

    Depositor

    Andrea Califano

    Article

    Tan et al bioRxiv 2021.06.28.449297

    Insert

    Cas9m4-KRAB-MeCP2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJMP1337

    Plasmid

    #119270

    Purpose

    for cloning new sgRNAs

    Depositor

    Carol Gross, Oren Rosenberg, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRISPRi_Mxi1_yl

    Plasmid

    #91248

    Purpose

    CRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertion

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

    Inserts

    Codon optimized dCas9-Mxi1
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SCR1'-tRNA and UAS1B8-TEF(136)

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pdCas9-humanized

    Plasmid

    #44246

    Purpose

    Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    dead Cas9 with 3X NLS
    

    Use

    CRISPR and Retroviral; Vector

    Tags

    3x NLS

    Expression

    Mammalian

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    MSCV LTR promoter

    Available since

    April 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRMC2-msfGFP

    Plasmid

    #194913

    Purpose

    Tetracycline inducible expression of msfGFP in Staphylococci

    Depositor

    Rikke Meyer

    Article

    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

    Insert

    Shine Dalgarno Sequence followed by monomeric superfolder GFP
    

    Use

    Tetracycline-inducible expression vector for stap…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV CMV-dSaCas9-KRAB-bGHpA

    Plasmid

    #106219

    Purpose

    AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

    Insert

    dead S. aureus Cas9 KRAB
    

    Use

    AAV and CRISPR

    Tags

    HA-NLS

    Expression

    Mammalian

    Mutation

    D10A and N580A

    Promoter

    CMV

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. psfGFP-LAMP1-mCherry (pHLARE)

    Plasmid

    #164477

    Purpose

    Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.

    Depositor

    Diane Barber

    Article

    Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Tags

    mCherry and superfolder GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGreen1

    Plasmid

    #138418

    Purpose

    CAPTURE2.0 vector containing dCas9-CBio, IRES and zsGreen1

    Depositor

    Jian Xu

    Article

    Liu et al Genome Biol. 2020 Mar 5;21(1):59. doi: 1

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    BioTAP-tag

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. TfR-sfGFP-myc tag-SpyCatcher003

    Plasmid

    #133451

    Purpose

    Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    TfR-sfGFP-myc tag-SpyCatcher003
    

    Use

    Tags

    GSSGS and myc tag

    Expression

    Mammalian

    Mutation

    Contains C20 and A23 mutations that improve plasm…

    Promoter

    CMV

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLX303-ABI1-dCas9

    Plasmid

    #154474

    Purpose

    Expresses ABI1 fused to the N terminus of dCas9

    Depositor

    Mikko Taipale

    Article

    Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

    Insert

    ABI1 (ABI1 Mustard Weed)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Includes ABI1 aa 126-423, D143A

    Promoter

    CMV

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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