We narrowed to 2,328 results for: dap

1. pPB-AMBRA1-501*-3xFLAG

   Plasmid

   #174160

   Purpose

   PiggyBac vector to stably express 3xFLAG-tagged AMBRA1 truncated at amino acid 501

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   AMBRA1 (AMBRA1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Missing aa 501-1208

   Promoter

   EF1A

   Available since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB-AMBRA1-217*-3xFLAG

   Plasmid

   #174161

   Purpose

   PiggyBac vector to stably express 3xFLAG-tagged AMBRA1 truncated at amino acid 217

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   AMBRA1 (AMBRA1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Missing aa 217-1208

   Promoter

   EF1A

   Available since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBIG1a_StrepII-Hook3_FTS_FHIP1B

   Plasmid

   #222299

   Purpose

   Co-expresses full-length human FHF (FTS, StrepII-HOOK3 and FHIP1B) in a pBIG1a vector

   Depositor

   Andrew Carter

   Article

   Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

   Inserts

   HOOK3 (HOOK3 Human)
   FTS (AKTIP Human)
   FHIP1B (FHIP1B Human)
   

   Use

   Tags

   StrepII-Psc

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBIG1a_StrepII-Hook3[451-718]_FTS_FHIP1B

   Plasmid

   #222300

   Purpose

   Co-expresses truncated human FHF (FTS, StrepII-HOOK3[451-718] and FHIP1B) in a pBIG1a vector

   Depositor

   Andrew Carter

   Article

   Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

   Inserts

   HOOK3[451-718] (HOOK3 Human)
   FTS (AKTIP Human)
   FHIP1B (FHIP1B Human)
   

   Use

   Tags

   StrepII-Psc

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBIG1a_StrepII-Hook3[571-718]_FTS_FHIP1B

   Plasmid

   #222301

   Purpose

   Co-expresses truncated human FHF (FTS, StrepII-HOOK3[571-718] and FHIP1B) in a pBIG1a vector

   Depositor

   Andrew Carter

   Article

   Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

   Inserts

   HOOK3[571-718] (HOOK3 Human)
   FTS (AKTIP Human)
   FHIP1B (FHIP1B Human)
   

   Use

   Tags

   StrepII-Psc

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHD-dsRed-attP_dCrk_flankingHRs

   Plasmid

   #131141

   Purpose

   Contains ~1kb homology regions flanking Drosophila crk locus. Used to make crk null mutant. Homology regions cloned into AarI and SapI sites of pHD-dsRed-attP (Harrison, O'Connor-Giles, Wildonger).

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Inserts

   Crk Upstream Homology Region (Crk Fly)
   Crk Downstream Homology Region (Crk Fly)
   

   Use

   CRISPR and Cre/Lox; Homology directed repair temp…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAMS823 His6-MBP-3C-ORF2p (238-1061, ORFeus-Hs) in pET41

   Plasmid

   #213024

   Purpose

   Expresses human LINE-1 ORF2p Core (238-1061) in E. coli as an N-MBP fusion

   Depositor

   Oliver Weichenrieder, Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   LINE-1 ORF2p (238-1061)
   

   Use

   Tags

   His6-MBP-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7 / Lac

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promoter) in pCEP4 Puro

   Plasmid

   #213030

   Purpose

   Expresses full length human LINE-1 in human cells (native L1RP sequence), with a C-terminal 3xFlag tag on ORF2

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Human LINE-1 (L1RP Sequence) with 5' UTR and ORF2-3xFlag
   

   Use

   Tags

   3xFlag (ORF2p)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRT006.3 L1 dual-luciferase AI retrotransposition reporter

   Plasmid

   #213031

   Purpose

   Bi-directional luciferase antisense intron (firefly fluc AI) human LINE-1 retrotransposition reporter (ORFeus-Hs sequence) for sleeping beauty integration

   Depositor

   Martin S. Taylor, Donna Romero

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Tet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker
   

   Use

   Luciferase

   Tags

   Firefly luciferase antisense intron (3' UTR)

   Expression

   Mammalian

   Mutation

   Promoter

   pTRE bidirectional tet-on

   Available since

   July 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

    Plasmid

    #213026

    Purpose

    Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
    

    Use

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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