We narrowed to 5,092 results for: codon optimized

1. pRSFDuet-gst-xr

   Plasmid

   #214761

   Purpose

   Xylose reductase from Hypocrea jecorina fused with GST-tagged and codon optimized for expression in E. coli

   Depositor

   Pimchai Chaiyen

   Article

   Jaroensuk et al J Biol Chem. 2023 Dec 28:105598. doi: 10

   Insert

   glutathione S-transferases fused with xylose reductase
   

   Use

   Tags

   Glutathione S-transferases

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. FUW-crRNA1and2-U6_TRE-dLbCpf1-NLS-hCTCF_WT-HA-P2A-tagBFP

   Plasmid

   #194885

   Purpose

   Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-WT and crRNA-1 and -2 targeting the human MECP2 locus.

   Depositor

   Shawn Liu

   Article

   Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

   Inserts

   dLbCpf1-NLS-hCTCF_WT
   U6-crRNA1and2
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Human codon optimized catalytically inactive LbCp…

   Promoter

   TRE and U6

   Available since

   Jan. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBUN6A11

   Plasmid

   #50579

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   dCas9-VP64
   gRNA scaffold
   

   Use

   CRISPR; Plant expression

   Tags

   3xFLAG, HA, and NLS

   Expression

   Mutation

   dCas9 = defective in DNA cleavage; 4×minimal VP16…

   Promoter

   OsU3p and Ubi1p

   Available since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. Cas12j-8-puro-crRNA

   Plasmid

   #194965

   Purpose

   The plasmid expresses human codon-optimized Cas12j-8, crRNA expression elements and puromycin resistence gene.

   Depositor

   Yongming Wang

   Article

   Wang et al Sci Adv. 2023 Feb 10;9(6):eabo6405. doi:

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBUN6I11

   Plasmid

   #50580

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   dCas9-KRAB
   gRNA scaffold
   

   Use

   CRISPR; Plant expression

   Tags

   3xFLAG, HA, and NLS

   Expression

   Mutation

   dCas9 that is defective in DNA cleavage; the maiz…

   Promoter

   OsU3p and Ubi1p

   Available since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK5-FLAG-SLC38A9.1

   Plasmid

   #71855

   Purpose

   overexpression

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Jan 9;347(6218):188-94. do

   Insert

   SLC38A9.1 (SLC38A9 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   CMV

   Available since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. SpCas9-NG-APOBEC-nCas9-Ung

   Plasmid

   #198539

   Purpose

   Expresses human codon-optimized SpCas9-NG-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NG-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NG-APOBEC-nCas9-Ung
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSH-EFIRES-P-AtAFB2-mCherry-weak NLS

   Plasmid

   #129717

   Purpose

   AAVS1 safe harbor targeting vector expressing AtAFB2-mCherry-weak NLS

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Insert

   AFB2 (AFB2 Mustard Weed)
   

   Use

   CRISPR and TALEN; Human safe harbor locus (a avs1…

   Tags

   mCherry-weak NLS

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   EF1a

   Available since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC001 - huLshC2C2-MBP for bacterial expression

   Plasmid

   #79150

   Purpose

   Expresses human codon-optimized LshC2c2 for purification in E. coli

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

   Insert

   C2c2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFastBac1 human full-length CENP-E-mNeon-6xHis

    Plasmid

    #180484

    Purpose

    pFastBac1 human CENP-E-mNeon-His, containing a 3c protease cleavage site between mNeon and His tag. Codon optimized for expression in Sf9 cells

    Depositor

    Julie Welburn

    Article

    Craske et al Open Biol. 2022 Mar;12(3):210389. doi: 1

    Insert

    CENP-E (CENPE Synthetic, Human, human)
    

    Use

    Tags

    mNeon-6xHis

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGES401

    Plasmid

    #190198

    Purpose

    For CRISPR-Cas9 mediated multiple gene editing in soybean, single transcript unit system driven by soybean elongation factor 1A promoter (pM4), Basta selection

    Depositor

    Yuefeng Guan

    Article

    Bai et al Mol Plant. 2022 Jul 4;15(7):1081-1083. d

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    3xFlag

    Expression

    Plant

    Mutation

    plant-codon optimized

    Promoter

    Glycine max elongation factor 1A(pM4)

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLX307-VHL(C162F)-3F-BirA

    Plasmid

    #220149

    Purpose

    Expresses VHL(C162F)-BirA as a control for VHL substrate identification by E-STUB

    Depositor

    William Sellers

    Article

    Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

    Insert

    von Hippel-Lindau tumor suppressor (VHL Human)
    

    Use

    Lentiviral

    Tags

    3F-BirA

    Expression

    Mammalian

    Mutation

    changed cysteine 162 to phenylalanine; codon opti…

    Promoter

    EF-1a

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. ORF3a-EGFP

    Plasmid

    #165121

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    ORF3a (ORF3a codon optimized SARS-COV-2)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Nsp7-mCherry

    Plasmid

    #165134

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp7 (ORF1ab codon optimized SARS-COV-2)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEN114 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-PuroR-bpA-Frt-Rosa26

    Plasmid

    #92143

    Purpose

    Targeting vector to introduce an osTir1 expressing cassette at the mouse Rosa26 locus using Puromycin selection. Auxin-inducible degron system.

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    osTir1
    

    Use

    Mouse Targeting

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJJGL001

    Plasmid

    #180605

    Purpose

    Plasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasX

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Insert

    DpbCasX
    

    Use

    CRISPR

    Tags

    10x His and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV.pU1a-SpCas9

    Plasmid

    #121507

    Purpose

    Expresses codon-optimized SpCas9 in mammalian cells.

    Depositor

    Guangping Gao

    Article

    Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

    Insert

    SpCas9
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    U1a

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET42b-ABE7.10

    Plasmid

    #120398

    Purpose

    Expresses ABE7.10-NLS with an N-terminal His Tag (His6) for bacterial expression

    Depositor

    Junjiu Huang

    Article

    Liang et al Nat Commun. 2019 Jan 8;10(1):67. doi: 10

    Insert

    ABE7.10
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Mammalian codon-optimized

    Promoter

    T7

    Available since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+)-HLA-cmyc-optiT1R3 a21-852

    Plasmid

    #113949

    Purpose

    mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R3 (TAS1R3 Human)
    

    Use

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    residues 21-852

    Promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3/er-(n2)oxStayGold(c4)

    Plasmid

    #185822

    Purpose

    A soluble photostable fluorescent marker of the ER lumen. The StayGold gene has no codon optimization for mammalian expression.

    Depositor

    Atsushi Miyawaki

    Article

    Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

    Insert

    er-(n2)oxStayGold(c4)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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