We narrowed to 14,981 results for: ef1;

1. pCDH-CMV-CD19 puro

   Plasmid

   #196634

   Purpose

   Express CD19

   Depositor

   Nicolas Manel

   Article

   Jeremiah et al J Exp Med. 2023 May 1;220(5):e20220666.

   Insert

   CD19 (CD19 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. FL-fibulin-3-V5-lentiviral-GFP

   Plasmid

   #29703

   Depositor

   Mariano Viapiano

   Article

   Hu et al Mol Cancer Res. 2009 Nov . 7(11):1756-70

   Insert

   EFEMP1 (EFEMP1 Human)
   

   Use

   Lentiviral

   Tags

   His and V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 23, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pLKO5.sgRNA.EFS.PAC

   Plasmid

   #57825

   Purpose

   Lentiviral Vector for Sp sgRNA delivery without SpCas9, Puromycin resistance, EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   sgRNA
   EFS
   PAC
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. CuO-LacI-NLS–SNAPf

   Plasmid

   #227368

   Purpose

   Lentiviral expression of LacI–SNAP

   Depositor

   David Pellman

   Article

   Papathanasiou et al Nature. 2023 Jul;619(7968):184-192. doi:

   Insert

   LacI NLS SNAPf
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJAM1.20_PB-NEDD4L_deltaC2-PGK-Blast

   Plasmid

   #205565

   Purpose

   Express human NEDD4L (delta C2 isoform)

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   NEDD4L (NEDD4L Human)
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   delta C2 isoform

   Promoter

   EF1

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDH_Str-KDEL_ManII-SBP-mCherry

   Plasmid

   #65259

   Purpose

   synchronize trafficking of ManII from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Streptavidin-KDEL and truncated Mannosidase II
   

   Use

   Lentiviral

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mutation

   contains only amino acids 1 to 116 of ManII

   Promoter

   CMV

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLGR002 LGR Cas9 guide vector

   Plasmid

   #188320

   Purpose

   Universal backbone for CRISPR/Cas9 sgRNA expression

   Depositor

   Laralynne Przybyla

   Article

   Heo et al Genome Biol. 2024 Jan 19;25(1):25. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   PuroR-T2A-mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   pU6 5'CMV and EF1-alpha

   Available since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDH_Str-KDEL_ManII-SBP-EGFP

   Plasmid

   #65258

   Purpose

   synchronize trafficking of ManII from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Streptavidin-KDEL and truncated Mannosidase II
   

   Use

   Lentiviral

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mutation

   contains only amino acids 1 to 116 of ManII

   Promoter

   CMV

   Available since

   Aug. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. HSC1-HS4-GiP

   Plasmid

   #58540

   Purpose

   Derived from HSC1-GiP where 2 copies of the HS4 insulator core are cloned inside the 3'LTR

   Depositor

   James Ellis

   Article

   Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

   Inserts

   Enhanced Green Fluorescence Protein, Puromycin resistance gene
   Core HS4 insulator sequence (dimer)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human EF1-alpha

   Available since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFH2.107_PB-EPN24-MCP_p6-T2A-eGFP-PGK-Blast

    Plasmid

    #205552

    Purpose

    Express EPN24-MCP-p6-T2A-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN24-MCP-p6
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAS_FLAG-G1 PylRS

    Plasmid

    #154763

    Purpose

    plasmid with G1 PylRS for amber suppression in transient or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAS_4xMx PylT_FLAG-Mx PylRS YA

    Plasmid

    #140012

    Purpose

    Plasmid with 4xMx PylT cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    Mx1201 PylRS YA (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y126A

    Promoter

    EF1

    Available since

    June 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE N-mNeonGreen (from SARS-CoV-2) IRES puro

    Plasmid

    #170467

    Purpose

    The plasmid codes for the Nucleoprotein (N) of SARS-CoV-2 fused to a mNeonGreen fluorescent protein in its C-terminus. Inserted in a lentiviral backbone. Puromycin resistance

    Depositor

    Raphael Gaudin

    Article

    Bakhache et al bioRxiv 2021

    Insert

    Nucleocapsid
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDH-CMV-MSI1(FL)

    Plasmid

    #60358

    Purpose

    express mouse MSI1 in mammalian cells

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (FL) (Msi1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    CMV

    Available since

    Nov. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLPB2-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid

    #187945

    Purpose

    dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    SpdCas9-tagRFPt-P2A-tagBFP
    

    Use

    Synthetic Biology; Piggybac

    Tags

    NLS, NLS + HA-tag, P2A, tagBFP, and tagRFPt

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAS_4xMma PylT(UUA)_FLAG-AcKRS

    Plasmid

    #140010

    Purpose

    Plasmid with 4xMmaP cassette and Mma PylRS for ochre (TAA) suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    AcKRS-3 (pylS Methanosarcina mazei)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    L301M, L305I, Y306F, L309A, C348F

    Promoter

    EF1

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE N-mRuby3 (from SARS-CoV-2) IRES puro

    Plasmid

    #170466

    Purpose

    The plasmid codes for the Nucleoprotein (N) of SARS-CoV-2 fused to a mRuby3 fluorescent protein in its C-terminus. Inserted in a lentiviral backbone. Puromycin resistance

    Depositor

    Raphael Gaudin

    Article

    Bakhache et al bioRxiv 2021

    Insert

    Nucleocapsid
    

    Use

    Lentiviral

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAS_4xMx PylT C41CA_FLAG_Mx PylRS

    Plasmid

    #140013

    Purpose

    Plasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    Mx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAS_4xMx PylT C41CA_FLAG_Mx PylRS YA

    Plasmid

    #140014

    Purpose

    Plasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    Mx1201 PylRS YA (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y126A, Y197F

    Promoter

    EF1

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFH2.102_PB-EPN11-MCP_I-T2A-eGFP-PGK-Blast

    Plasmid

    #205547

    Purpose

    Express EPN11-MCP-I-T2A-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN11-MCP-I
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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