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GFP-FBXL11

Plasmid

#126543

Purpose

Expresses GFP-tagged FBXL11 in mammalian cells

Depositor

Michele Pagano

Article

Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

Insert

FBXL11 (KDM2A Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

LTR

Available Since

July 3, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR P2R-P3-mKate2

Plasmid

#48345

Purpose

Contributes the coding sequence for the mKate2 fluorescent protein as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

Depositor

Anna Planas, Tomas Santalucia

Article

Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

Insert

mKate2

Use

Gateway Cloning

Mutation

Contains a stop codon at the 3' end of the c…

Promoter

none

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Beclin1-(151-241)

Plasmid

#24393

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Beclin1 (BECN1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Amino acids 151-241 are deleted. Delta CC mutant.

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
SPYtag-Histag-Avidin

Plasmid

#214836

Purpose

This plasmid encodes a protein (SPYtag-Histag-Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

Depositor

Hironori Funabiki

Article

Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

Insert

SPYtag-Histag-Avidin

Expression

Bacterial

Promoter

T7

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pUAS-Cre

Plasmid

#50797

Purpose

Expression of Cre recombinase under the control of the UAS-promoter

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

Cre recombinase

Use

Multi-species

Promoter

UAS-hsp70

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-Cdc42-T17N

Plasmid

#12970

Depositor

Gary Bokoch

Insert

Cdc42 DN (CDC42 Human)

Tags

GST

Expression

Bacterial

Mutation

T17N Dominant negative

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only
pDONR223-FGR

Plasmid

#23877

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FGR (FGR Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-pri-let-7a-1

Plasmid

#51377

Depositor

Narry Kim

Article

Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

Insert

pri-let-7a-1 (MIRLET7A1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pEF6.HA-ARRDC3

Plasmid

#38317

Depositor

Quan Lu

Article

Nabhan et al EMBO Rep. 2010 Aug;11(8):605-11. Epub 20

Insert

ARRDC3 (ARRDC3 Human)

Tags

HA

Expression

Mammalian

Promoter

EF1

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pRK5-hGRK4

Plasmid

#32690

Depositor

Robert Lefkowitz

Article

Premont et al J Biol Chem. 1996 Mar 15;271(11):6403-10

Insert

GRK4 (GRK4 Human)

Expression

Mammalian

Available Since

Feb. 21, 2012

Availability

Academic Institutions and Nonprofits only
pGEX-2T-Rac1-T17N

Plasmid

#12978

Depositor

Gary Bokoch

Insert

Rac1 DN (RAC1 Human)

Tags

GST

Expression

Bacterial

Mutation

T17N Dominant negative

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3 Plk4(Sak) D154A (Nigg HR26)

Plasmid

#41164

Depositor

Erich Nigg

Article

Habedanck et al Nat Cell Biol. 2005 Nov;7(11):1140-6.

Insert

Plk4 D154A (PLK4 Human)

Tags

3xMyc and PreScission Site

Expression

Mammalian

Mutation

D154A

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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pAB07 (CK2beta)

Plasmid

#27091

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2beta (CSNK2B Human)

Tags

Myc

Expression

Mammalian

Available Since

May 17, 2012

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag LIMK2

Plasmid

#20513

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

LIMK2 (LIMK2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
HA-BirA*-pDEST-N-pcDNA5/FRT/TO

Plasmid

#118375

Purpose

Expression vector for BioID in mammalian system. Gateway compatible destination vector to generate stable, inducible cell line expressing N-terminally tagged HABirA* and protein of interest.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

HA-BirA*

Expression

Mammalian

Available Since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pET11a-SUMO2

Plasmid

#53142

Purpose

E.coli expression of untagged mature human SUMO2 (amino acids 1-92)

Depositor

Frauke Melchior

Article

Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

Insert

SUMO2 (SUMO3 Human)

Expression

Bacterial

Mutation

mature form (amino acids 1-92)

Promoter

T7

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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CAG-EnR

Plasmid

#19715

Depositor

Connie Cepko

Article

Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

Insert

engrailed (en Fly)

Expression

Mammalian

Mutation

Drosophila engrailed repressor domain

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pBECKP-spe

Plasmid

#117236

Purpose

ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase

Depositor

Quanjiang Ji

Article

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Insert

ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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FKBP-C-TEV 219

Plasmid

#100096

Purpose

SNIPer plasmid, C terminal TEV fused to FKBP

Depositor

Jim Wells

Article

Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112

Insert

C-TEV

Use

Retroviral

Tags

FKBP and Myc

Expression

Mammalian

Mutation

C terminal domain

Promoter

CMV

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS-CD4-myc

Plasmid

#58537

Purpose

Expresses human CD4 with a C-terminal myc tag in mammalian cells

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

CD4 (CD4 Human)

Tags

myc

Expression

Mammalian

Available Since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only