We narrowed to 5,110 results for: mcs

1. pINTO-N3::hTET3 V908L

   Plasmid

   #136370

   Purpose

   Expresses epitope-tagged human TET3 V908L variant in mammalian cells

   Depositor

   Roberto Bonasio

   Article

   Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

   Insert

   TET3 (TET3 Human)
   

   Tags

   2xStrep-tagII, FLAG, and HA

   Expression

   Mammalian

   Mutation

   V908L

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pINTO-N3::hTET3 V1089M

   Plasmid

   #136369

   Purpose

   Expresses epitope-tagged human TET3 V1089M variant in mammalian cells

   Depositor

   Roberto Bonasio

   Article

   Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

   Insert

   TET3 (TET3 Human)
   

   Tags

   2x Strep-tagII, FLAG, and HA

   Expression

   Mammalian

   Mutation

   V1089M

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pINTO-N3::hTET3 A1076T

   Plasmid

   #136368

   Purpose

   Expresses epitope-tagged human TET3 A1076C variant in mammalian cells

   Depositor

   Roberto Bonasio

   Article

   Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

   Insert

   TET3 (TET3 Human)
   

   Tags

   2xStrepTagII, FLAG, and HA

   Expression

   Mammalian

   Mutation

   A1076T

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pINTO-N3::hTET3 R752C

   Plasmid

   #136367

   Purpose

   Expresses epitope-tagged human TET3 R752C variant in mammalian cells

   Depositor

   Roberto Bonasio

   Article

   Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

   Insert

   TET3 (TET3 Human)
   

   Tags

   2xStrepTagII, FLAG, and HA

   Expression

   Mammalian

   Mutation

   R752C

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pINTO-N3::hTET3 F1072C

   Plasmid

   #136366

   Purpose

   Expresses epitope-tagged human TET3 F1072C variant in mammalian cells

   Depositor

   Roberto Bonasio

   Article

   Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

   Insert

   TET3 (TET3 Human)
   

   Tags

   2xStrepTagII, FLAG, and HA

   Expression

   Mammalian

   Mutation

   F1072C

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-FLAG-RPS14(89-151)

   Plasmid

   #122299

   Purpose

   expresses a part of RPS14 protein with a flag tag

   Depositor

   Gerardo Ferbeyre

   Article

   Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

   Insert

   RPS14 (RPS14 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deleted amino acids 1 to 88

   Promoter

   CMV

   Available Since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV-RPS14(WT)-Myc

   Plasmid

   #122236

   Purpose

   expresses RPS14 protein with a Myc tag in mammalian cells (hygro resistance)

   Depositor

   Gerardo Ferbeyre

   Article

   Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

   Insert

   RPS14 (RPS14 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-FLAG-RPS14(2-88)

   Plasmid

   #122298

   Purpose

   expresses a part of RPS14 protein with a flag tag

   Depositor

   Gerardo Ferbeyre

   Article

   Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

   Insert

   RPS14 (partial) (RPS14 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deleted amino acids 89 to 151

   Promoter

   CMV

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CAG-DIO-ChR2(H134R)-eYFP (AAV PHP.eB)

   Viral Prep

   #127090-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from pAAV-CAG-DIO-ChR2(H134R)-eYFP (#127090). In addition to the viral particles, you will also receive purified pAAV-CAG-DIO-ChR2(H134R)-eYFP plasmid DNA. CAG-driven, Cre-dependent expression of humanized channelrhodopsin H134R mutant fused to EYFP, for optogenetic activation. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Viviana Gradinaru

   Article

   Rajendran et al Nat Commun. 2019 Apr 26;10(1):1944. doi:

   Promoter

   CAG

   Tags

   EYFP (Cre-dependent)

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

10. pAAV-CAG-DIO-PPO-Venus (AAV5)

    Viral Prep

    #139504-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-CAG-DIO-PPO-Venus (#139504). In addition to the viral particles, you will also receive purified pAAV-CAG-DIO-PPO-Venus plasmid DNA. CAG-driven, Cre-dependent expression of Venus-tagged parapinopsin. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Michael Bruchas

    Article

    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

    Promoter

    CAG

    Tags

    Venus

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

11. pGRG36

    Plasmid

    #16666

    Depositor

    Nancy Craig

    Article

    McKenzie et al BMC Microbiol. 2006 Apr 28;6:39. doi: 10

    Insert

    mTn7::MCS
    

    Expression

    Bacterial

    Available Since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGRG25

    Plasmid

    #16665

    Depositor

    Nancy Craig

    Article

    McKenzie et al BMC Microbiol. 2006 Apr 28;6:39. doi: 10

    Insert

    mTn7::MCS
    

    Expression

    Bacterial

    Available Since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVITRO1-SS-288

    Plasmid

    #68788

    Purpose

    N Trans V2

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Insert

    N-Terminal Sensor 1 V2
    

    Tags

    NLS and flag

    Expression

    Mammalian

    Available Since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVITRO1-SS-257

    Plasmid

    #68774

    Purpose

    C Trans V2

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Insert

    C-terminal Sensor 1 V2
    

    Tags

    NLS and flag

    Expression

    Mammalian

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTE-10M0X

    Plasmid

    #20318

    Depositor

    Dirk Schnappinger

    Article

    Klotzsche et al Nucleic Acids Res. 2009 Apr;37(6):1778-8

    Insert

    Pimyc-tetR#10
    

    Expression

    Bacterial

    Available Since

    March 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC-0_v6

    Plasmid

    #124426

    Purpose

    Full MCS, lacks araC & P(BAD) (step 2)

    Depositor

    Yolanda Schaerli

    Article

    Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGYL1_pPromALB_FireflyLuc

    Plasmid

    #220282

    Purpose

    Firefly luciferase with minimal Albumin promoter without enhancer; enhancer of interest can be cloned into MCS

    Depositor

    David Waxman

    Article

    Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

    Type

    Empty backbone

    Use

    Luciferase

    Available Since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIA027

    Plasmid

    #200418

    Purpose

    amyE::(dronPA cm), for dronPA fusions. PALM-compatible integration vector for Bacillus subtilis. Includes dronPA photoswitchable FP with multi cloning sites (MCS) for fast and interchangeable cloning.

    Depositor

    Rut Carballido-López

    Article

    Altinoglu et al bioRxiv 2023

    Type

    Empty backbone

    Tags

    dronPA

    Expression

    Bacterial

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pC-0_v4

    Plasmid

    #124424

    Purpose

    Full MCS, lacks araC & P(BAD) (step 2)

    Depositor

    Yolanda Schaerli

    Article

    Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJQ200uc1

    Plasmid

    #78502

    Purpose

    Suicide vector for gene replacement, modified MCS to maximise utility of insert restriction sites.

    Depositor

    Michael Hynes

    Article

    Quandt et al Gene. 1993 May 15;127(1):15-21.

    Type

    Empty backbone

    Use

    Suicide vector for gene replacement

    Tags

    none

    Promoter

    none

    Available Since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only